LOCUS       sequence-107589-        1072 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  CrBlue  fused to mcherry on the C terminus.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1072)
  AUTHORS   Karunarathne WK, Giri L, Kalyanaraman V, Gautam N
  TITLE     Optically triggering spatiotemporally confined GPCR activity in a 
            cell and programming neurite initiation and extension.
  JOURNAL   Proc Natl Acad Sci U S A. 2013 Apr 23;110(17):E1565-74. doi: 
            10.1073/pnas.1220697110. Epub 2013 Mar 11.
  PUBMED    23479634
REFERENCE   2  (bases 1 to 1072)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1073/pnas.1220697110"; journalName: "Proc Natl Acad Sci U S A"; 
            date: "2013-04-23- 23"; volume: "110"; issue: "17"; pages: 
            "E1565-74"
COMMENT     Sequence Label: CrBlue-mcherry - pcDNA3.1
FEATURES             Location/Qualifiers
     source          1..1072
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     complement(329..348)
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     primer_bind     598..616
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     regulatory      758..767
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     gap             1038..1048
                     /estimated_length=11
ORIGIN
        1 gggnannagg gcgntcgagg ctgatcagcg ggtttaacgg gccctctaga ttacttgtac
       61 agctcgtcca tgccgccggt ggagtggcgg ccctcggcgc gttcgtactg ttccacgatg
      121 gtgtagtcct cgttgtggga ggggatgtcc aacttgatgt tgacgttgta ggcgccgggc
      181 agctgcacgg gcttcttggc cttgtaggtg gtcttgacct cagcgtcgta gtggccgccg
      241 tccttcagct tcagcctctg cttgatctcg cccttcaggg cgccgtcctc ggggtacatc
      301 cgctcggagg aggcctccca gcccatggtc ttcttctgca ttacggggcc gtcggagggg
      361 aagttggtgc cgcgcagctt caccttgtag atgaactcgc cgtcctgcag ggaggagtcc
      421 tgggtcacgg tcaccacgcc gccgtcctcg aagttcatca cgcgctccca cttgaagccc
      481 tcggggaagg acagcttcaa gtagtcgggg atgtcggcgg ggtgcttcac gtaggccttg
      541 gagccgtaca tgaactgagg ggacaggatg tcccaggcga agggcagggg gccacccttg
      601 gtcaccttca gcttggcggt ctgggtgccc tcgtagggcg gtnctcgctc tcgnctcnat
      661 ctcgaactcg ttggccgttc acggagccct ccatgtgcac cttgaagcgc atgaactcct
      721 tgatgatggc catggtatcc tcctccgccc ttgctcacca tggcggccgc agcnnagcca
      781 ccttggaggt cttcggttag ntcaacagcg tactgnnnng catggcatta ccaccgacaa
      841 ttctttccnc aaacacccnn gtaatacagt ctttggaact gcttgttncn tgaggcatnn
      901 atnngnnnnn tgnnnnnnca nncggantnn ngaanacnan gnnttntcca cngnnnnatt
      961 ctaanntnnn ntgtgtntcn nnnncantgn aaagnncctn gggnnngnnc acnnanagng
     1021 nccnatnccg cncgtttnnn nnnnnnnngc tnnatcnnng nagcncctgg at
//
