LOCUS       sequence-107591-         553 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  based on pDe-CAS9, encodes Cas9 D10A nickase variant.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 553)
  AUTHORS   Fauser F, Schiml S, Puchta H
  TITLE     Both CRISPR/Cas-based nucleases and nickases can be used efficiently
            for genome engineering in Arabidopsis thaliana.
  JOURNAL   Plant J. 2014 Jul;79(2):348-59. doi: 10.1111/tpj.12554. Epub 2014 
            Jun 17.
  PUBMED    24836556
REFERENCE   2  (bases 1 to 553)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1111/tpj.12554";
            journalName: "Plant J"; date: "2014-07"; volume: "79"; issue: "2"; 
            pages: "348-59"
COMMENT     Sequence Label: pDe-CAS9-D10A
FEATURES             Location/Qualifiers
     source          1..553
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    complement(52..176)
                     /gene="mutant version of attR"
                     /label=attR2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
ORIGIN
        1 ggggaatata aatgtcaggc tcccttatac acagccagtc tgcaggtcga ccatagtgac
       61 tggatatgtt gtgttttaca gtattatgta gtctgttttt tatgcaaaat ctaatttaat
      121 atattgatat ttatatcatt ttacgtttct cgttcanctt tcttgtacaa agtggttcga
      181 taattccgat ccagcctagg cccgggcctg aggacgcgtc catggttaat taagacgtcc
      241 ggaccgacta gtggatcctc tanagtcgac ctgcaggcat gcaagcttca gcttgccaac
      301 atggtggagc acgacactct cgtctactcc aagaatatca aagatacagt ctcagaagac
      361 caaagggcta ttgagacttt tcaacaaagg gtaatatcgg gaaacctcct cggattccat
      421 tgcccagcta tctgtcactt catcaaaagg acagtagaaa aggaaggtgg cacctacaaa
      481 tgccatcatt gcgataaagg aaaggctatc gttcaagatg cctctgccga cagtggtccc
      541 aaagatggac ccc
//
