LOCUS       sequence-107594-         683 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Binary expression vector with A.th. codon-optimized Cas9 and Gateway
            destination sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 683)
  AUTHORS   Fauser F, Schiml S, Puchta H
  TITLE     Both CRISPR/Cas-based nucleases and nickases can be used efficiently
            for genome engineering in Arabidopsis thaliana.
  JOURNAL   Plant J. 2014 Jul;79(2):348-59. doi: 10.1111/tpj.12554. Epub 2014 
            Jun 17.
  PUBMED    24836556
REFERENCE   2  (bases 1 to 683)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1111/tpj.12554";
            journalName: "Plant J"; date: "2014-07"; volume: "79"; issue: "2"; 
            pages: "348-59"
COMMENT     Sequence Label: pDe-CAS9
FEATURES             Location/Qualifiers
     source          1..683
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    complement(99..223)
                     /gene="mutant version of attR"
                     /label=attR2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
ORIGIN
        1 cagccaccgc gaaaatgaca tcaaaaacgc cattaacctg atgttctggg gaatataaat
       61 gtcaggctcc cttatacaca gccagtctgc aggtcgacca tagtgactgg atatgttgtg
      121 ttttacagta ttatgtagtc tgttttttat gcaaaatcta atttaatata ttgatattta
      181 tatcatttta cgtttctcgt tcagctttct tgtacaaagt ggttcgataa ttccgatcca
      241 gcctaggccc gggcctgagg acgcgtccat ggttaattaa gacgtccgga ccgactagtg
      301 gatcctctag agtcgacctg caggcatgca agcttcttcg tcaacatggt ggagcacgac
      361 acgcttgtct actccaaaaa tatcaaagat acagtctcag aagaccaaag ggcaattgag
      421 acttttcaac aaagggtaat atccggaaac ctcctcggat tccattgccc agctatctgt
      481 cactttattg tgaagatagt ggaaaaggaa ggtggctcct acaaatgcca tcattgcgat
      541 aaaggaaagg ccatcgttga agatgcctct gccgacagtg gtcccaaaga tggaccccca
      601 cccacgagga gcatcgtgga aaaagaagac gttccaacca cgtcttcaaa gcaagtggat
      661 tgatgtgata tctccactga cgt
//
