LOCUS       sequence-107595-         924 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  a binary Agrobacterium vector carrying a modified HSVtk gene as a 
            negative selection marker against ectopic transformants. Uses 
            Gateway cloning..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 924)
  AUTHORS   Khang CH, Park SY, Lee YH, Kang S
  TITLE     A dual selection based, targeted gene replacement tool for 
            Magnaporthe grisea and Fusarium oxysporum.
  JOURNAL   Fungal Genet Biol. 2005 Jun;42(6):483-92. Epub 2005 Apr 25.
  PUBMED    15893252
REFERENCE   2  (bases 1 to 924)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Fungal 
            Genet Biol."; date: "2005-06"; volume: "42(6)"; pages: "483-92. Epub
            2005 Apr 25"
COMMENT     Sequence Label: pGKO2-Gateway
FEATURES             Location/Qualifiers
     source          1..924
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        complement(52..154)
                     /label=cat promoter
                     /note="promoter of the E. coli cat gene encoding 
                     chloramphenicol acetyltransferase"
     protein_bind    complement(299..530)
                     /gene="mutant version of attP"
                     /label=attP2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     primer_bind     complement(549..565)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(550..566)
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     primer_bind     complement(590..609)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(591..609)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(619..636)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(619..635)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(628..650)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
ORIGIN
        1 atgccattgg gatatatcaa cggtggtata tccagtgatt tttttctcca ttttagcttc
       61 cttagctcct gaaaatctcg ataactcaaa aaatacgccc ggtagtgatc ttatttcatt
      121 atggtgaaag ttggaacctc ttacgtgccg atcaacgtct cattttcgcc aaaagttggc
      181 ccagggcttc ccggtatcaa cagggacacc aggatttatt tattctgcga agtgatcttc
      241 cgtcacaggt atttattcgg cgcaaagtgc gtcgggtgat gctgccaact tagtcgacta
      301 caggtcacta ataccatcta agtagttgat tcatagtgac tggatatgtt gtgttttaca
      361 gtattatgta gtctgttttt tatgcaaaat ctaatttaat atattgatat ttatatcatt
      421 ttacgtttct cgttcagctt tcttgtacaa agttggcatt ataagaaagc attgcttatc
      481 aatttgttgc aacgaacagg tcactatcag tcaaaataaa atcattattt gccatccagc
      541 aagcttatcg ataccgtcga cctcgagggg gggcccggta cccaattcgc cctatagtga
      601 gtcgtattac gcgcgctcac tggccgtcgt tttacaacgt cgtgactggg aaaaccctgg
      661 cgttacccaa cttaatcgcc ttgcagcaca tccccctttc gccagctggc gtaatagcga
      721 agaggcccgc accgatcgcc cttcccaaca gttgcgcagc ctgaatggcg aatggaaatt
      781 gtaagcgtta atgtttgcat taatgaatcg gccaacgcgc ggggagaggc ggtttgcgta
      841 ttggctagag cagcttgcca atcgagtttc tccataataa tgtgtgagta gttcccagat
      901 aagggaatta gggttcctat aggg
//
