LOCUS       sequence-107598-         860 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Binary expression vector with A.th. codon-optimized Cas9 for 
            conventional cloning.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 860)
  AUTHORS   Fauser F, Schiml S, Puchta H
  TITLE     Both CRISPR/Cas-based nucleases and nickases can be used efficiently
            for genome engineering in Arabidopsis thaliana.
  JOURNAL   Plant J. 2014 Jul;79(2):348-59. doi: 10.1111/tpj.12554. Epub 2014 
            Jun 17.
  PUBMED    24836556
REFERENCE   2  (bases 1 to 860)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1111/tpj.12554";
            journalName: "Plant J"; date: "2014-07"; volume: "79"; issue: "2"; 
            pages: "348-59"
COMMENT     Sequence Label: pCAS9-TPC
FEATURES             Location/Qualifiers
     source          1..860
                     /mol_type="genomic DNA"
                     /organism="unspecified"
ORIGIN
        1 gtatgtatat atgtagatct ggactttttg gagttgttga cttgattgta tttgtgtgtg
       61 tatatgtgtg ttctgatctt gatatgttat gtatgtgcag cgaattcggc gcgccatgga
      121 taagaagtac tctatcggac tcgatatcgg aactaactct gtgggatggg ctgtgatcac
      181 cgatgagtac aaggtgccat ctaagaagtt caaggttctc ggaaacaccg ataggcactc
      241 tatcaagaaa aaccttatcg gtgctctcct cttcgattct ggtgaaactg ctgaggctac
      301 cagactcaag agaaccgcta gaagaaggta caccagaaga aagaacagga tctgctacct
      361 ccaagagatc ttctctaacg agatggctaa agtggatgat tcattcttcc acaggctcga
      421 agagtcattc ctcgtggaag aagataagaa gcacgagagg caccctatct tcggaaacat
      481 cgttgatgag gtggcatacc acgagaagta ccctactatc taccacctca gaaagaagct
      541 cgttgattct actgataagg ctgatctcag gctcatctac ctcgctctcg ctcacatgat
      601 caagttcaga ggacacttcc tcatcgaggg tgatctcaac cctgataact ctgatgtgga
      661 taagttgttc atccagctcg tgcagaccta caaccagctt ttcgaagaga accctatcaa
      721 cgcttcaggt gtggatgcta aggctatcct ctctgctagg ctctctaagt caagaaggct
      781 tgagaacctc attgctcagc tccctggtga gaagaagaac ggacttttcg gaaacttgat
      841 cgctctctct ctcggactca
//
