LOCUS       sequence-107599-         751 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Lentivirus expressing shRNA to GFP.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 751)
  AUTHORS   Goodwin JM, Svensson RU, Lou HJ, Winslow MM, Turk BE, Shaw RJ
  TITLE     An AMPK-independent signaling pathway downstream of the LKB1 tumor 
            suppressor controls Snail1 and metastatic potential.
  JOURNAL   Mol Cell. 2014 Aug 7;55(3):436-50. doi: 
            10.1016/j.molcel.2014.06.021. Epub 2014 Jul 17.
  PUBMED    25042806
REFERENCE   2  (bases 1 to 751)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.molcel.2014.06.021"; journalName: "Mol Cell"; date: 
            "2014-08-7- 7"; volume: "55"; issue: "3"; pages: "436-50"
COMMENT     Sequence Label: pLentiX2-PURO-shGFP
FEATURES             Location/Qualifiers
     source          1..751
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    206..230
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    362..380
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     primer_bind     complement(426..445)
                     /label=H1
                     /note="Human H1 promoter, forward primer"
     protein_bind    complement(623..647)
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
ORIGIN
        1 catgacccgc aagcccggtg cctgacgccc gccccacgac ccgcagcgcc cgaccgaaag
       61 gagcgcacga ccccatgcat ctcgagggcc cggtaccttt aagaccaatg acttacaagg
      121 cagctgtaga tcttagccac tttttaaaag aaaagggggg actggaaggg ctagactcga
      181 gcggccgcca ctgtgctgga tatcaaccac tttgtacaag aaagctgggt ctagatatct
      241 cgagtgcggc cgcgaattcc accacactgg actagtggat ccgagctcgg taccaagctt
      301 ttccaaaaag aagcagcacg acttcttctc tcttgaagaa gaagtcgtgc tgcttcgggg
      361 atctctatca ctgataggga acttataaga ttcccaaatc caaagacatt tcacgtttat
      421 ggtgatttcc cagaacacat agcgacatgc aaatattgca gggcgccact cccctgtccc
      481 tcacagccat cttcctgcca gggcgcacgc gcgctgggtg ttcccgccta gtgacactgg
      541 gcccgcgatt ccttggagcg ggttgatgac gtcagcgttc gaattcggta ccggatccag
      601 tcgactgaat tggttccttt aaagcctgct tttttgtaca aacttgttga tatctgcaga
      661 attccaccac actggactag ctcactccca acgaagacaa gatctgcttt ttgcttgtac
      721 tgggtctctc tggttagacc agatctgagc c
//
