LOCUS       sequence-107600-         862 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  based on pDe-CAS9, encodes Cas9 D10A nickase variant.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 862)
  AUTHORS   Fauser F, Schiml S, Puchta H
  TITLE     Both CRISPR/Cas-based nucleases and nickases can be used efficiently
            for genome engineering in Arabidopsis thaliana.
  JOURNAL   Plant J. 2014 Jul;79(2):348-59. doi: 10.1111/tpj.12554. Epub 2014 
            Jun 17.
  PUBMED    24836556
REFERENCE   2  (bases 1 to 862)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1111/tpj.12554";
            journalName: "Plant J"; date: "2014-07"; volume: "79"; issue: "2"; 
            pages: "348-59"
COMMENT     Sequence Label: pDe-CAS9-D10A
FEATURES             Location/Qualifiers
     source          1..862
                     /mol_type="genomic DNA"
                     /organism="unspecified"
ORIGIN
        1 cgtatgtata tatgtagatc tggacttttt ggagttgttg acttgattgt atttgtgtgt
       61 gtatatgtgt gttctgatct tgatatgtta tgtatgtgca gcgaattcgg cgcgccatgg
      121 ataagaagta ctctatcgga ctcgctatcg gaactaactc tgtgggatgg gctgtgatca
      181 ccgatgagta caaggtgcca tctaagaagt tcaaggttct cggaaacacc gataggcact
      241 ctatcaagaa aaaccttatc ggtgctctcc tcttcgattc tggtgaaact gctgaggcta
      301 ccagactcaa gagaaccgct agaagaaggt acaccagaag aaagaacagg atctgctacc
      361 tccaagagat cttctctaac gagatggcta aagtggatga ttcattcttc cacaggctcg
      421 aagagtcatt cctcgtggaa gaagataaga agcacgagag gcaccctatc ttcggaaaca
      481 tcgttgatga ggtggcatac cacgagaagt accctactat ctaccacctc agaaagaagc
      541 tcgttgattc tactgataag gctgatctca ggctcatcta cctcgctctc gctcacatga
      601 tcaagttcag aggacacttc ctcatcgagg gtgatctcaa ccctgataac tctgatgtgg
      661 ataagttgtt catccagctc gtgcagacct acaaccagct tttcgaagag aaccctatca
      721 acgcttcagg tgtggatgct aaggctatcc tctctgctag gctctctaag tcaagaaggc
      781 ttgagaacct cattgctcag ctccctggtg agaagaagaa cggacttttc ggaaacttga
      841 tcgctctctc tctcggactc ac
//
