LOCUS       sequence-107601-         748 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Lentivirus expressing shRNA to human LKB1.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 748)
  AUTHORS   Goodwin JM, Svensson RU, Lou HJ, Winslow MM, Turk BE, Shaw RJ
  TITLE     An AMPK-independent signaling pathway downstream of the LKB1 tumor 
            suppressor controls Snail1 and metastatic potential.
  JOURNAL   Mol Cell. 2014 Aug 7;55(3):436-50. doi: 
            10.1016/j.molcel.2014.06.021. Epub 2014 Jul 17.
  PUBMED    25042806
REFERENCE   2  (bases 1 to 748)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.molcel.2014.06.021"; journalName: "Mol Cell"; date: 
            "2014-08-7- 7"; volume: "55"; issue: "3"; pages: "436-50"
COMMENT     Sequence Label: pLentiX2-PURO-shLKB1-Hu
FEATURES             Location/Qualifiers
     source          1..748
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    206..230
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    363..381
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     primer_bind     complement(427..446)
                     /label=H1
                     /note="Human H1 promoter, forward primer"
     protein_bind    complement(624..648)
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
ORIGIN
        1 catgacccgc aagcccggtg cctgacgccc gccccacgac ccgcagcgcc cgaccgaaag
       61 gagcgcacga ccccatgcat ctcgagggcc cggtaccttt aagaccaatg acttacaagg
      121 cagctgtaga tcttagccac tttttaaaag aaaagggggg actggaaggg ctagactcga
      181 gcggccgcca ctgtgctgga tatcaaccac tttgtacaag aaagctgggt ctagatatct
      241 cgagtgcggc cgcgaattcc accacactgg actagtggat ccgagctcgg taccaagctt
      301 ttccaaaaac gaagagaagc agaaaatggg acagcacacc attttctgct tctcttcggg
      361 gatctctatc actgataggg aacttataag attcccaaat ccaaagacat ttcacgttta
      421 tggtgatttc ccagaacaca tagcgacatg caaatattgc agggcgccac tcccctgtcc
      481 ctcacagcca tcttcctgcc agggcgcacg cgcgctgggt gttcccgcct agtgacactg
      541 ggcccgcgat tccttggagc gggttgatga cgtcagcgtt cgaattcggt accggatcca
      601 gtcgactgaa ttggttcctt taaagcctgc ttttttgtac aaacttgttg atatctgcag
      661 aattccacca cactggacta gctcactccc aacgaagaca agatctgctt tttgcttgta
      721 ctgggtctct ctggttagac cagatctg
//
