LOCUS       sequence-107602-         713 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Binary expression vector with A.th. codon-optimized Cas9 and Gateway
            destination sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 713)
  AUTHORS   Fauser F, Schiml S, Puchta H
  TITLE     Both CRISPR/Cas-based nucleases and nickases can be used efficiently
            for genome engineering in Arabidopsis thaliana.
  JOURNAL   Plant J. 2014 Jul;79(2):348-59. doi: 10.1111/tpj.12554. Epub 2014 
            Jun 17.
  PUBMED    24836556
REFERENCE   2  (bases 1 to 713)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1111/tpj.12554";
            journalName: "Plant J"; date: "2014-07"; volume: "79"; issue: "2"; 
            pages: "348-59"
COMMENT     Sequence Label: pDe-CAS9
FEATURES             Location/Qualifiers
     source          1..713
                     /mol_type="genomic DNA"
                     /organism="unspecified"
ORIGIN
        1 tatatgtgtg ttctgatctt gatatgttat gtatgtgcag cgaattcggc gcgccatgga
       61 taagaagtac tctatcggac tcgatatcgg aactaactct gtgggatggg ctgtgatcac
      121 cgatgagtac aaggtgccat ctaagaagtt caaggttctc ggaaacaccg ataggcactc
      181 tatcaagaaa aaccttatcg gtgctctcct cttcgattct ggtgaaactg ctgaggctac
      241 cagactcaag agaaccgcta gaagaaggta caccagaaga aagaacagga tctgctacct
      301 ccaagagatc ttctctaacg agatggctaa agtggatgat tcattcttcc acaggctcga
      361 agagtcattc ctcgtggaag aagataagaa gcacgagagg caccctatct tcggaaacat
      421 cgttgatgag gtggcatacc acgagaagta ccctactatc taccacctca gaaagaagct
      481 cgttgattct actgataagg ctgatctcag gctcatctac ctcgctctcg ctcacatgat
      541 caagttcaga ggacacttcc tcatcgaggg tgatctcaac cctgataact ctgatgtgga
      601 taagttgttc atccagctcg tgcagaccta caaccagctt ttcgaagaga accctatcaa
      661 cgcttcaggt gtggatgcta aggctatcct ctctgctagg ctctctaagt caa
//
