LOCUS       sequence-107607-        8145 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Bicaudal D protein aa1-594 (excluding start Met) with N-terminal 
            flag tag and C-terminal FKBP.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8145)
  AUTHORS   Bentley M, Decker H, Luisi J, Banker G
  TITLE     A novel assay reveals preferential binding between Rabs, kinesins, 
            and specific endosomal subpopulations.
  JOURNAL   J Cell Biol. 2015 Feb 2;208(3):273-281. Epub 2015 Jan 26.
  PUBMED    25624392
REFERENCE   2  (bases 1 to 8145)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Cell 
            Biol."; date: "2015-02-2"; volume: "208(3)"; pages: "273-281. Epub 
            2015 Jan 26"
COMMENT     Sequence Label: pBa-flag-BicD2 594-FKBP
FEATURES             Location/Qualifiers
     source          1..8145
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        1..276
                     /label=chicken beta-actin promoter
     primer_bind     1209..1231
                     /label=pCAGGS-5
                     /note="Chimeric intron in CAG promoter, forward primer"
     CDS             1299..1322
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     CDS             3126..3446
                     /codon_start=1
                     /product="human FK506-binding protein FKBP12"
                     /label=FKBP
                     /translation="GVQVETISPGDGRTFPKRGQTCVVHYTGMLEDGKKFDSSRDRNKP
                     FKFMLGKQEVIRGWEEGVAQMSVGQRAKLTISPDYAYGATGHPGIIPPHATLVFDVELL
                     KLE"
     polyA_signal    3566..3699
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     3656..3675
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(3731..3747)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(3731..3747)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(3744..3766)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    3755..3771
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(3779..3785,3786..3803,3804..3809))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    3824..3845
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(3962..3979)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(4133..4721)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(4213..4232)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(4892..5683,5684..5752))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     5515..5534
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(5753..5857)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     5925..5943
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     primer_bind     complement(5981..6003)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     6103..6122
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     6316..6338
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     6330..6347
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     6331..6347
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
ORIGIN
        1 tcgaggtgag ccccacgttc tgcttcactc tccccatctc ccccccctcc ccacccccaa
       61 ttttgtattt atttattttt taattatttt gtgcagcgat gggggcgggg gggggggggg
      121 cgcgcgccag gcggggcggg gcggggcgag gggcggggcg gggcgaggcg gagaggtgcg
      181 gcggcagcca atcagagcgg cgcgctccga aagtttcctt ttatggcgag gcggcggcgg
      241 cggcggccct ataaaaagcg aagcgcgcgg cgggcgggag tcgctgcgtt gccttcgccc
      301 cgtgccccgc tccgcgccgc ctcgcgccgc ccgccccggc tctgactgac cgcgttactc
      361 ccacaggtga gcgggcggga cggcccttct cctccgggct gtaattagcg cttggtttaa
      421 tgacggctcg tttcttttct gtggctgcgt gaaagcctta aagggctccg ggagggccct
      481 ttgtgcgggg gggagcggct cggggggtgc gtgcgtgtgt gtgtgcgtgg ggagcgccgc
      541 gtgcggcccg cgctgcccgg cggctgtgag cgctgcgggc gcggcgcggg gctttgtgcg
      601 ctccgcgtgt gcgcgagggg agcgcggccg ggggcggtgc cccgcggtgc gggggggctg
      661 cgaggggaac aaaggctgcg tgcggggtgt gtgcgtgggg gggtgagcag ggggtgtggg
      721 cgcggcggtc gggctgtaac ccccccctgc acccccctcc ccgagttgct gagcacggcc
      781 cggcttcggg tgcggggctc cgtgcggggc gtggcgcggg gctcgccgtg ccgggcgggg
      841 ggtggcggca ggtgggggtg ccgggcgggg cggggccgcc tcgggccggg gagggctcgg
      901 gggaggggcg cggcggcccc ggagcgccgg cggctgtcga ggcgcggcga gccgcagcca
      961 ttgcctttta tggtaatcgt gcgagagggc gcagggactt cctttgtccc aaatctggcg
     1021 gagccgaaat ctgggaggcg ccgccgcacc ccctctagcg ggcgcgggcg aagcggtgcg
     1081 gcgccggcag gaaggaaatg ggcggggagg gccttcgtgc gtcgccgcgc cgccgtcccc
     1141 ttctccatct ccagcctcgg ggctgccgca gggggacggc tgccttcggg ggggacgggg
     1201 cagggcgggg ttcggcttct ggcgtgtgac cggcggggtt tatatcttcc cttctctgtt
     1261 cctccgcagc ccccaagctt ggcgcgccta ggatcatgga ttacaaggat gacgatgaca
     1321 aatctcattc cgcgccgtcg gaggaggagg agtatgcgcg tctggtgatg gaggcgcagc
     1381 cggagtggct gcgcgccgag gtgaagcggc tgtcccacga gctggccgag accacgcgtg
     1441 agaagatcca ggcggccgag tatggactgg cggtgctgga ggagaagcac cagctcaagc
     1501 tgcagttcga ggagctcgag gtggactatg aggccatccg cagcgagatg gagcagctca
     1561 aagaggcatt tggccaggca catacaaacc acaagaaggt ggctgctgat ggtgagagcc
     1621 gggaggagag cctgatccag gagtcggcct ccaaggagca gtactacgtg cggaaggtgc
     1681 tggagctgca gacagagctg aaacagctgc gcaacgtcct caccaacacc cagtctgaga
     1741 atgagcgcct cacgtctgtg gcccaggagc taaaagagat caaccagaat gtggagatcc
     1801 agcgtggtcg cctgcgggat gacatcaagg agtacaagtt ccgggaggcc cgtctacttc
     1861 aggactactc agagctggag gaggagaaca tcagcctgca gaaacaagtg tctgtgctca
     1921 ggcagaacca ggtggagttt gagggcctca agcatgaaat caagcgcctg gaggaggaga
     1981 cagagtacct caacagccag ctagaggatg ccatccggct taaggagatc tctgaacggc
     2041 agttggagga ggcgctggag acactgaaga cagagcggga gcagaagaac aacctgcgca
     2101 aggagttgtc gcactacatg agcatcaacg attccttcta taccagccac ctgcaggtct
     2161 ccttagatgg cctcaagttc agtgatgata ctgtcaccgc agagcccaac aatgacgccg
     2221 aagccctggt caatggcttt gagcacagtg gcttggtcaa atcatccttg gacaacaaga
     2281 catccacacc caggaaggat ggcctagctc caccctcccc cagcctcgtc tctgacctgc
     2341 tcagtgagct ccacatatct gagatccaga agctgaaaca gcagctggtg cagatggagc
     2401 gggagaaggt gggcctgctg gcaacactgc aggacacaca gaagcagctg gagcaggctc
     2461 ggggaaccct ctctgagcag catgagaagg tgaatcgtct cacagagaac cttagtgccc
     2521 tccggcgcct gcaggctggc aaggagcgac agacctcgtt ggataatgag aaggaccgtg
     2581 acagccacga agacggtgac tactatgagg tggacatcaa tgggcctgag atcctggcct
     2641 gcaagtacca cgtggctgtg gctgaggctg gcgagctccg ggagcagctc aaggcgttgc
     2701 gcagcacaca cgaagctcgc gaagcccagc acgcagaaga aaagggccgg tatgaggctg
     2761 agggccaggc actcactgag aagatctctc tgctggagaa ggctagccac caggaccgag
     2821 agctgctggc ccatctggaa aaggagctga agaaggtgag cgatgtggct ggtgagaccc
     2881 agggcagcct gaatgtggct caggatgagc tggtgacctt cagcgaggag ctggccaacc
     2941 tctaccacca tgtgtgcatg tgcaacaacg agacgcccaa ccgtgtcatg ctcgactatt
     3001 atcgtgaggg ccagggcaag gctggccgca ccagcccaga gggccgcggg cgccggtcac
     3061 ctgtcctctt gcccaagggg ctgttggcca cagaggtggg ccgagcagat cggaccgtct
     3121 ctagaggagt gcaggtggaa accatctccc caggagacgg gcgcaccttc cccaagcgcg
     3181 gccagacctg cgtggtgcac tacaccggga tgcttgaaga tggaaagaaa tttgattcct
     3241 cccgggacag aaacaagccc tttaagttta tgctaggcaa gcaggaggtg atccgaggct
     3301 gggaagaagg ggttgcccag atgagtgtgg gtcagagagc caaactgact atatctccag
     3361 attatgccta tggtgccact gggcacccag gcatcatccc accacatgcc actctcgtct
     3421 tcgatgtgga gcttctaaaa ctggaaacta gttattgagg atcataatca gccatatcac
     3481 atctgtagag gttttacttg ctttaaaaaa cctcccacac ctccccctga acctgaaaca
     3541 taaaatgaat gcaattgttg ttgttaactt gtttattgca gcttataatg gttacaaata
     3601 aagcaatagc atcacaaatt cacaaataaa gcattttttt cactgcattc tagttgtggt
     3661 ttgtccaaac tcatcaatgt atcttatcat gtctggatcc ccgggtaccg agctcgaatt
     3721 cgtaatcatg gtcatagctg tttcctgtgt gaaattgtta tccgctcaca attccacaca
     3781 acatacgagc cggaagcata aagtgtaaag cctggggtgc ctaatgagtg agctaactca
     3841 cattaattgc gttgcgctca ctgcccgctt tccagtcggg aaacctgtcg tgccagctgc
     3901 attaatgaat cggccaacgc gcggggagag gcggtttgcg tattgggcgc tcttccgctt
     3961 cctcgctcac tgactcgctg cgctcggtcg ttcggctgcg gcgagcggta tcagctcact
     4021 caaaggcggt aatacggtta tccacagaat caggggataa cgcaggaaag aacatgtgag
     4081 caaaaggcca gcaaaaggcc aggaaccgta aaaaggccgc gttgctggcg tttttccata
     4141 ggctccgccc ccctgacgag catcacaaaa atcgacgctc aagtcagagg tggcgaaacc
     4201 cgacaggact ataaagatac caggcgtttc cccctggaag ctccctcgtg cgctctcctg
     4261 ttccgaccct gccgcttacc ggatacctgt ccgcctttct cccttcggga agcgtggcgc
     4321 tttctcatag ctcacgctgt aggtatctca gttcggtgta ggtcgttcgc tccaagctgg
     4381 gctgtgtgca cgaacccccc gttcagcccg accgctgcgc cttatccggt aactatcgtc
     4441 ttgagtccaa cccggtaaga cacgacttat cgccactggc agcagccact ggtaacagga
     4501 ttagcagagc gaggtatgta ggcggtgcta cagagttctt gaagtggtgg cctaactacg
     4561 gctacactag aaggacagta tttggtatct gcgctctgct gaagccagtt accttcggaa
     4621 aaagagttgg tagctcttga tccggcaaac aaaccaccgc tggtagcggt ggtttttttg
     4681 tttgcaagca gcagattacg cgcagaaaaa aaggatctca agaagatcct ttgatctttt
     4741 ctacggggtc tgacgctcag tggaacgaaa actcacgtta agggattttg gtcatgagat
     4801 tatcaaaaag gatcttcacc tagatccttt taaattaaaa atgaagtttt aaatcaatct
     4861 aaagtatata tgagtaaact tggtctgaca gttaccaatg cttaatcagt gaggcaccta
     4921 tctcagcgat ctgtctattt cgttcatcca tagttgcctg actccccgtc gtgtagataa
     4981 ctacgatacg ggagggctta ccatctggcc ccagtgctgc aatgataccg cgagacccac
     5041 gctcaccggc tccagattta tcagcaataa accagccagc cggaagggcc gagcgcagaa
     5101 gtggtcctgc aactttatcc gcctccatcc agtctattaa ttgttgccgg gaagctagag
     5161 taagtagttc gccagttaat agtttgcgca acgttgttgc cattgctaca ggcatcgtgg
     5221 tgtcacgctc gtcgtttggt atggcttcat tcagctccgg ttcccaacga tcaaggcgag
     5281 ttacatgatc ccccatgttg tgcaaaaaag cggttagctc cttcggtcct ccgatcgttg
     5341 tcagaagtaa gttggccgca gtgttatcac tcatggttat ggcagcactg cataattctc
     5401 ttactgtcat gccatccgta agatgctttt ctgtgactgg tgagtactca accaagtcat
     5461 tctgagaata gtgtatgcgg cgaccgagtt gctcttgccc ggcgtcaata cgggataata
     5521 ccgcgccaca tagcagaact ttaaaagtgc tcatcattgg aaaacgttct tcggggcgaa
     5581 aactctcaag gatcttaccg ctgttgagat ccagttcgat gtaacccact cgtgcaccca
     5641 actgatcttc agcatctttt actttcacca gcgtttctgg gtgagcaaaa acaggaaggc
     5701 aaaatgccgc aaaaaaggga ataagggcga cacggaaatg ttgaatactc atactcttcc
     5761 tttttcaata ttattgaagc atttatcagg gttattgtct catgagcgga tacatatttg
     5821 aatgtattta gaaaaataaa caaatagggg ttccgcgcac atttccccga aaagtgccac
     5881 ctgacgtcta agaaaccatt attatcatga cattaaccta taaaaatagg cgtatcacga
     5941 ggccctttcg tctcgcgcgt ttcggtgatg acggtgaaaa cctctgacac atgcagctcc
     6001 cggagacggt cacagcttgt ctgtaagcgg atgccgggag cagacaagcc cgtcagggcg
     6061 cgtcagcggg tgttggcggg tgtcggggtt ggcttaacta tgcggcatca gagcagattg
     6121 tactgagagt gcaccatatg cggtgtgaaa taccgcacag atgcgtaagg agaaaatacc
     6181 gcatcaggcg ccattcgcca ttcaggctgc gcaactgttg ggaagggcga tcggtgcggg
     6241 cctcttcgct attacgccag ctggcgaaag ggggatgtgc tgcaaggcga ttaagttggg
     6301 taacgccagg gttttcccag tcacgacgtt gtaaaacgac ggccagtgcc aagttgggat
     6361 ctttgcattg gcccacggct ctcaggatgg ggatgctccc cttcagcacc cggttcccct
     6421 tggaaactga tggtcctggc tctgtggcat ggcagtggca ctgtgaggag cccctaccag
     6481 cagcacacag tgggtttggc actgccacgc tccggatgcc gcgctctgat ccaaccccat
     6541 aatcaaggga acccgaattg ccccatcatt gcccccacca cccccatcct gccgggccct
     6601 cacaccccac gctgccttgt ggtgacattc cccagcccaa acccacggct tcatggctac
     6661 cgcggggcat ttcccattgc cgccccatta tcagctctgc acacctcccg ctgtacccat
     6721 gcctcgtggc tgcccttctt tgacgtataa tcttctaatt aatacccggc cttgtcaaag
     6781 tggagcacaa acgttaatta attccccagc aggcaggtaa ttaacagtgt gactcccttt
     6841 ttgctgcgag tggggctgat acagagagat gtggcactat ggagcccacg gggtcctggc
     6901 actgggtgcc cacggaggtc cccatgtgct gcagtgtcac cgcctccgag gtgacagtat
     6961 tgtccctgcg gtgtccctgc agctcagctc tgtccacagg gccacctcca gtttggaggg
     7021 gacacaatgc agccccgatg caacccatcc tcgcagcatc ccagggacaa agaccccact
     7081 gcaagaccgc acacagggct gggtcccgct cccctaatat ctacagtgct tttgcatggc
     7141 cccttaatca atgcagttaa tcagcatgcg ctcatgcacc gctctggagc tgcaaagccc
     7201 ctcgcagcgc tgctcaccaa caccgcgcac cgccccggcc cagcctgcag cacgcgctgc
     7261 aaacaggaaa gaaacaaaat attgcccaaa tgtaggcaaa ggcattcggc tgccttgacc
     7321 tccgccgggc cgggccctgc ctgactcagc tccttactca gcgctcgctt cctccctccg
     7381 gctgccaccg ccgcagcgca caccctgaca aagagtggcc cttaacgggc tctgaggtgc
     7441 acccagcagt gcactcagca gtccaagggc cggcctggag gtttgcaccg ctacgtgctg
     7501 acattagcat tgaacttggc cctgggtagt gctgcaggcc gggcggggtg ggtgtagaga
     7561 gtgcagcgcg cgttgcaccc ggtgcccctt cccctccctt gcatcccagc aggctgcacc
     7621 ccagcaccag gcccgtgcat gcatgctcct ggtgttattg cagcctggtg catgcatgcg
     7681 tcttagtggt gcagcgctgt gcatgcatcc tccttggtgt gtagcagctt agtgcatgca
     7741 tacccctcgg tgttattgct gctctgtgca cgcacgctca ttgtatcact tcatcccagt
     7801 gcatgcactc acactggagc gattgctgct cggtgcacgc acactcattg tatcacgtca
     7861 gctcagtggc tgcacgcaca ccggtgttat tgctgctcgg tgcgtgcatg cacatcagtg
     7921 tcgctgcagc tcagtgcatg cacgctcatt gcccatcgct atccctgcct ctcctgctgg
     7981 cgctccccgg gaggtgactt caaggggacc gcaggaccac ctcgggggtg gggggagggc
     8041 tgcacacgcg gaccccgctc cccctcccca acaaagcact gtggaatcaa aaagggggga
     8101 ggggggatgg aggggcgcgt cacacccccg ccccacaccc tcacc
//
