LOCUS       sequence-107610-         966 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses Fok1 nuclease domain fused to catalytically inactive Cas9 
            DNA-binding domain in mammalian cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 966)
  AUTHORS   Guilinger JP, Thompson DB, Liu DR
  TITLE     Fusion of catalytically inactive Cas9 to FokI nuclease improves the 
            specificity of genome modification.
  JOURNAL   Nat Biotechnol. 2014 Apr 25. doi: 10.1038/nbt.2909.
  PUBMED    24770324
REFERENCE   2  (bases 1 to 966)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Biotechnol. 2014 Apr 25. doi: 10.1038/nbt.2909."
COMMENT     Sequence Label: FokI-dCas9
FEATURES             Location/Qualifiers
     source          1..966
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(12..29)
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
ORIGIN
        1 gtttaaactc aatggtgatg gtgatgatga ccggtcagtc acccccaagc tgtgacaaat
       61 ctatccgagt ttcatataat cccgtgatgg attggtgaat cagtgtcgcg tctagcacct
      121 ccttggtaga agtgtatcgt ttgcgatcta tcgttgtgtc aaaatacttg aatgcggctg
      181 gagcgccgag gttggtaaga gtaaacaaat ggataatatt ttccgcctgc tcacgtatgg
      241 gtttatccct gtgcttgttg tatgcgctta atactttgtc cagattggca tcagctagga
      301 tgactctctt actgaattcc gaaatttgct ctatgatttc gtcgagataa tgtttgtgct
      361 gctcaacaaa aagttgcttc tgttcgttat cttcaggtga acctttcaac ttctcgtaat
      421 gggacgctaa atacaggaaa ttcacgtatt tagacggtag tgcgagttcg ttcccctttt
      481 gaagctctcc ggcgctagcc aacatccgtt ttcggccatt ttctaactca aacagactat
      541 actttggtag tttaattatg agatcctttt ttacttcctt gtaacctttc gcctcaagga
      601 agtcgatggg gttcttttca aaagacgagc gctccataat cgttatcccc aataattctt
      661 tgactgactt cagtttcttg gattttccct tctcaacttt tgccactact aggacagaat
      721 aggcaactgt agggctatcg aagccaccgt actttttcgg gtcccagtcc tttttacgag
      781 cgatgagctt atcactattc ctttttggaa gaatcgattc ctttgaaaac cctccggtct
      841 gcacctcagt tttctttact atgttgactt ggggcatgga caaaactttt ctcaccgtcg
      901 cgaagtcccg gcccttatcc catacgattt cacctgtctc cccattggtt tcaattaaag
      961 gtcgtt
//
