LOCUS       sequence-107622-         659 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  PLtetO-1:cstAlead::mCherry, PLlacO-1:RBS(st7):csrA
             .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 659)
  TITLE     Lim Lab plasmids
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 659)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: pHL1745
FEATURES             Location/Qualifiers
     source          1..659
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    153..169
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     terminator      complement(224..310)
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     primer_bind     complement(617..636)
                     /label=mCherry-F
                     /note="mCherry, forward primer"
ORIGIN
        1 cagaaacttc cttcggggca tttacgccaa tacgtacctg gcttgccctt tacccctaaa
       61 actgtcacgg tgacctcatc cccaatcatg agggtctcac caactcgacg agtcagaatc
      121 agcatttttt ttcctcctta cccgggagta tcttgttatc cgctcacaat gtcaatgtta
      181 tccgctcaca tttatggatc cgcgcctagg tctagggcgg cggatttgtc ctactcagga
      241 gagcgttcac cgacaaacaa cagataaaac gaaaggccca gtctttcgac tgagcctttc
      301 gttttatttg atgcctctag cacgcgtacc atgggccctt acttgtacag ctcgtccatg
      361 ccgccggtgg agtggcggcc ctcggcgcgt tcgtactgtt ccacgatggt gtagtcctcg
      421 ttgtgggagg tgatgtccaa cttgatgttg acgttgtagg cgccgggcag ctgcacgggc
      481 ttcttggcct tgtaggtggt cttgacctca gcgtcgtagt ggccgccgtc cttcagcttc
      541 agcctctgct tgatctcgcc cttcagggcg ccgtcctcgg ggtacatccg ctcggaggag
      601 gcctcccagc ccatggtctt cttctgcatt acggggccgt cggaggggaa gttggtgcc
//
