LOCUS       sequence-107623-         739 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  PLtetO-1:glgClead::mCherry, PLlacO-1:RBS(st7):csrA
             .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 739)
  TITLE     Lim Lab plasmids
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 739)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: pHL1722
FEATURES             Location/Qualifiers
     source          1..739
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     terminator      264..350
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     protein_bind    405..421
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
ORIGIN
        1 cggcgccctg aagggcgaga tcaagcagag gctgaagctg aaggacggcg gccactacga
       61 cgctgaggtc aagaccacct acaaggccaa gaagcccgtg cagctgcccg gcgcctacaa
      121 cgtcaacatc aagttggaca tcacctccca caacgaggac tacaccatcg tggaacagta
      181 cgaacgcgcc gagggccgcc actccaccgg cggcatggac gagctgtaca agtaagggcc
      241 catggtacgc gtgctagagg catcaaataa aacgaaaggc tcagtcgaaa gactgggcct
      301 ttcgttttat ctgttgtttg tcggtgaacg ctctcctgag taggacaaat ccgccgccct
      361 agacctaggc gcggatccat aaatgtgagc ggataacatt gacattgtga gcggataaca
      421 agatactccc gggtaaggag gaaaaaaaat gctgattctg actcgtcgag ttggtgagac
      481 cctcatgatt ggggatgagg tcaccgtgac agttttaggg gtaaagggca accaggtacg
      541 tattggcgta aatgccccga aggaagtttc tgttcaccgt gaagagatct accagcgtat
      601 ccaggctgaa aaatcccagc agtccagtta ctaaggtacc tgaaagaagc tttaatcgta
      661 cagggtagta caaataaaaa aggcacgtca gatgacgtgc cttttttctt gtgagcagtg
      721 aattcggata tattccgct
//
