LOCUS       sequence-107625-        2470 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  mCherry fused between Cry2 and gamma 9 to study cell migration.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 2470)
  AUTHORS   O'Neill PR, Gautam N
  TITLE     Subcellular optogenetic inhibition of G proteins generates signaling
            gradients and cell migration.
  JOURNAL   Mol Biol Cell. 2014 Aug 1;25(15):2305-14. doi: 
            10.1091/mbc.E14-04-0870. Epub 2014 Jun 11.
  PUBMED    24920824
REFERENCE   2  (bases 1 to 2470)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1091/mbc.E14-04-0870"; journalName: "Mol Biol Cell"; date: 
            "2014-08-1- 1"; volume: "25"; issue: "15"; pages: "2305-14"
COMMENT     Sequence Label: Cry2 mcherry Gamma 9 in pcDNA3.1
FEATURES             Location/Qualifiers
     source          1..2470
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             29..1522
                     /codon_start=1
                     /product="N-terminal photolyase homology region of 
                     Arabidopsis thaliana cryptochrome 2"
                     /label=CRY2PHR
                     /note="binds to CIB1 upon exposure to blue light (Kennedy 
                     et al., 2010)"
                     /translation="MKMDKKTIVWFRRDLRIEDNPALAAAAHEGSVFPVFIWCPEEEGQ
                     FYPGRASRWWMKQSLAHLSQSLKALGSDLTLIKTHNTISAILDCIRVTGATKVVFNHLY
                     DPVSLVRDHTVKEKLVERGISVQSYNGDLLYEPWEIYCEKGKPFTSFNSYWKKCLDMSI
                     ESVMLPPPWRLMPITAAAEAIWACSIEELGLENEAEKPSNALLTRAWSPGWSNADKLLN
                     EFIEKQLIDYAKNSKKVVGNSTSLLSPYLHFGEISVRHVFQCARMKQIIWARDKNSEGE
                     ESADLFLRGIGLREYSRYICFNFPFTHEQSLLSHLRFFPWDADVDKFKAWRQGRTGYPL
                     VDAGMRELWATGWMHNRIRVIVSSFAVKFLLLPWKWGMKYFWDTLLDADLECDILGWQY
                     ISGSIPDGHELDRLDNPALQGAKYDPEGEYIRQWLPELARLPTEWIHHPWDAPLTVLKA
                     SGVELGTNYAKPIVDIDTARELLAKAISRTREAQIMIGAA"
     CDS             1532..2239
                     /codon_start=1
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /label=mCherry
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEG
                     TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF
                     EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK
                     GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
     primer_bind     complement(1677..1695)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     primer_bind     1945..1964
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     CDS             2249..2266
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
ORIGIN
        1 gctaccggac tcagatctcg aggccaccat gaagatggac aaaaagacta tagtttggtt
       61 tagaagagac ctaaggattg aggataatcc tgcattagca gcagctgctc acgaaggatc
      121 tgtttttcct gtcttcattt ggtgtcctga agaagaagga cagttttatc ctggaagagc
      181 ttcaagatgg tggatgaaac aatcacttgc tcacttatct caatccttga aggctcttgg
      241 atctgacctc actttaatca aaacccacaa cacgatttca gcgatcttgg attgtatccg
      301 cgttaccggt gctacaaaag tcgtctttaa ccacctctat gatcctgttt cgttagttcg
      361 ggaccatacc gtaaaggaga agctggtgga acgtgggatc tctgtgcaaa gctacaatgg
      421 agatctattg tatgaaccgt gggagatata ctgcgaaaag ggcaaacctt ttacgagttt
      481 caattcttac tggaagaaat gcttagatat gtcgattgaa tccgttatgc ttcctcctcc
      541 ttggcggttg atgccaataa ctgcagcggc tgaagcgatt tgggcgtgtt cgattgaaga
      601 actagggctg gagaatgagg ccgagaaacc gagcaatgcg ttgttaacta gagcttggtc
      661 tccaggatgg agcaatgctg ataagttact aaatgagttc atcgagaagc agttgataga
      721 ttatgcaaag aacagcaaga aagttgttgg gaattctact tcactacttt ctccgtatct
      781 ccatttcggg gaaataagcg tcagacacgt tttccagtgt gcccggatga aacaaattat
      841 atgggcaaga gataagaaca gtgaaggaga agaaagtgca gatctttttc ttaggggaat
      901 cggtttaaga gagtattctc ggtatatatg tttcaacttc ccgtttactc acgagcaatc
      961 gttgttgagt catcttcggt ttttcccttg ggatgctgat gttgataagt tcaaggcctg
     1021 gagacaaggc aggaccggtt atccgttggt ggatgccgga atgagagagc tttgggctac
     1081 cggatggatg cataacagaa taagagtgat tgtttcaagc tttgctgtga agtttcttct
     1141 ccttccatgg aaatggggaa tgaagtattt ctgggataca cttttggatg ctgatttgga
     1201 atgtgacatc cttggctggc agtatatctc tgggagtatc cccgatggcc acgagcttga
     1261 tcgcttggac aatcccgcgt tacaaggcgc caaatatgac ccagaaggtg agtacataag
     1321 gcaatggctt cccgagcttg cgagattgcc aactgaatgg atccatcatc catgggacgc
     1381 tcctttaacc gtactcaaag cttctggtgt ggaactcgga acaaactatg cgaaacccat
     1441 tgtagacatc gacacagctc gtgagctact agctaaagct atttcaagaa cccgtgaagc
     1501 acagatcatg atcggagcag cagcccagct tatggtgagc aagggcgagg aggataacat
     1561 ggccatcatc aaggagttca tgcgcttcaa ggtgcacatg gagggctccg tgaacggcca
     1621 cgagttcgag atcgagggcg agggcgaggg ccgcccctac gagggcaccc agaccgccaa
     1681 gctgaaggtg accaagggtg gccccctgcc cttcgcctgg gacatcctgt cccctcagtt
     1741 catgtacggc tccaaggcct acgtgaagca ccccgccgac atccccgact acttgaagct
     1801 gtccttcccc gagggcttca agtgggagcg cgtgatgaac ttcgaggacg gcggcgtggt
     1861 gaccgtgacc caggactcct ccctgcagga cggcgagttc atctacaagg tgaagctgcg
     1921 cggcaccaac ttcccctccg acggccccgt aatgcagaag aagaccatgg gctgggaggc
     1981 ctcctccgag cggatgtacc ccgaggacgg cgccctgaag ggcgagatca agcagaggct
     2041 gaagctgaag gacggcggcc actacgacgc tgaggtcaag accacctaca aggccaagaa
     2101 gcccgtgcag ctgcccggcg cctacaacgt caacatcaag ttggacatca cctcccacaa
     2161 cgaggactac accatcgtgg aacagtacga acgcgccgag ggccgccact ccaccggcgg
     2221 catggacgag ctgtacaagg gtaccatgca tcaccatcac catcacgccc aggatctcag
     2281 cgagaaggac ctgttgaaga tggaggtgga gcagctgaag aaagaagtga aaaacacaag
     2341 aattccgatt tccaaagcgg gaaaggaaat caaggagtac gtggaggccc aagcaggaaa
     2401 cgatcctttt ctcaaaggca tccctgagga caagaatccc ttcaaggaaa aaggcggttg
     2461 tataataagc
//
