LOCUS       sequence-107628-        9444 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  KIF5C motor protein aa1-559 with C-terminal tandem dimer Tomato and 
            FKBP tags.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9444)
  AUTHORS   Bentley M, Decker H, Luisi J, Banker G
  TITLE     A novel assay reveals preferential binding between Rabs, kinesins, 
            and specific endosomal subpopulations.
  JOURNAL   J Cell Biol. 2015 Feb 2;208(3):273-281. Epub 2015 Jan 26.
  PUBMED    25624392
REFERENCE   2  (bases 1 to 9444)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Cell 
            Biol."; date: "2015-02-2"; volume: "208(3)"; pages: "273-281. Epub 
            2015 Jan 26"
COMMENT     Sequence Label: pBa-KIF5C 559-tdTomato-FKBP
FEATURES             Location/Qualifiers
     source          1..9444
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        418..693
                     /label=chicken beta-actin promoter
     primer_bind     1626..1648
                     /label=pCAGGS-5
                     /note="Chimeric intron in CAG promoter, forward primer"
     CDS             2578..2589
                     /codon_start=1
                     /product="WELQut protease recognition and cleavage site"
                     /label=WELQut site
                     /translation="WELQ"
     CDS             3398..4825
                     /codon_start=1
                     /product="tandem dimeric (pseudo-monomeric) derivative of 
                     DsRed (Shaner et al., 2004)"
                     /label=tdTomato
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEEVIKEFMRFKVRMEGSMNGHEFEIEGEGEGRPYEGTQTA
                     KLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYKKLSFPEGFKWERVMNFEDGG
                     LVTVTQDSSLQDGTLIYKVKMRGTNFPPDGPVMQKKTMGWEASTERLYPRDGVLKGEIH
                     QALKLKDGGHYLVEFKTIYMAKKPVQLPGYYYVDTKLDITSHNEDYTIVEQYERSEGRH
                     HLFLGHGTGSTGSGSSGTASSEDNNMAVIKEFMRFKVRMEGSMNGHEFEIEGEGEGRPY
                     EGTQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYKKLSFPEGFKWERVM
                     NFEDGGLVTVTQDSSLQDGTLIYKVKMRGTNFPPDGPVMQKKTMGWEASTERLYPRDGV
                     LKGEIHQALKLKDGGRYLVEFKTIYMAKKPVQLPGYYYVDTKLDITSHNEDYTIVEQYE
                     RSEGRHHLFLYGMDELYK"
     CDS             4841..5161
                     /codon_start=1
                     /product="human FK506-binding protein FKBP12"
                     /label=FKBP
                     /translation="GVQVETISPGDGRTFPKRGQTCVVHYTGMLEDGKKFDSSRDRNKP
                     FKFMLGKQEVIRGWEEGVAQMSVGQRAKLTISPDYAYGATGHPGIIPPHATLVFDVELL
                     KLE"
     polyA_signal    5281..5415
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(5318..5337)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     5372..5391
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(5447..5463)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(5447..5463)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(5460..5482)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    5471..5487
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(5495..5501,5502..5519,5520..5525))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    5540..5561
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(5678..5695)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(5849..6437)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(5929..5948)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(6608..7399,7400..7468))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     7231..7250
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(7469..7573)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     7641..7659
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     primer_bind     complement(7697..7719)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     7819..7838
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     8032..8054
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     8046..8063
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     8047..8063
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
ORIGIN
        1 ttagtgcatg catacccctc ggtgttattg ctgctctgtg cacgcacgct cattgtatca
       61 cttcatccca gtgcatgcac tcacactgga gcgattgctg ctcggtgcac gcacactcat
      121 tgtatcacgt cagctcagtg gctgcacgca caccggtgtt attgctgctc ggtgcgtgca
      181 tgcacatcag tgtcgctgca gctcagtgca tgcacgctca ttgcccatcg ctatccctgc
      241 ctctcctgct ggcgctcccc gggaggtgac ttcaagggga ccgcaggacc acctcggggg
      301 tggggggagg gctgcacacg cggaccccgc tccccctccc caacaaagca ctgtggaatc
      361 aaaaaggggg gaggggggat ggaggggcgc gtcacacccc cgccccacac cctcacctcg
      421 aggtgagccc cacgttctgc ttcactctcc ccatctcccc cccctcccca cccccaattt
      481 tgtatttatt tattttttaa ttattttgtg cagcgatggg ggcggggggg gggggggcgc
      541 gcgccaggcg gggcggggcg gggcgagggg cggggcgggg cgaggcggag aggtgcggcg
      601 gcagccaatc agagcggcgc gctccgaaag tttcctttta tggcgaggcg gcggcggcgg
      661 cggccctata aaaagcgaag cgcgcggcgg gcgggagtcg ctgcgttgcc ttcgccccgt
      721 gccccgctcc gcgccgcctc gcgccgcccg ccccggctct gactgaccgc gttactccca
      781 caggtgagcg ggcgggacgg cccttctcct ccgggctgta attagcgctt ggtttaatga
      841 cggctcgttt cttttctgtg gctgcgtgaa agccttaaag ggctccggga gggccctttg
      901 tgcggggggg agcggctcgg ggggtgcgtg cgtgtgtgtg tgcgtgggga gcgccgcgtg
      961 cggcccgcgc tgcccggcgg ctgtgagcgc tgcgggcgcg gcgcggggct ttgtgcgctc
     1021 cgcgtgtgcg cgaggggagc gcggccgggg gcggtgcccc gcggtgcggg ggggctgcga
     1081 ggggaacaaa ggctgcgtgc ggggtgtgtg cgtggggggg tgagcagggg gtgtgggcgc
     1141 ggcggtcggg ctgtaacccc cccctgcacc cccctccccg agttgctgag cacggcccgg
     1201 cttcgggtgc ggggctccgt gcggggcgtg gcgcggggct cgccgtgccg ggcggggggt
     1261 ggcggcaggt gggggtgccg ggcggggcgg ggccgcctcg ggccggggag ggctcggggg
     1321 aggggcgcgg cggccccgga gcgccggcgg ctgtcgaggc gcggcgagcc gcagccattg
     1381 ccttttatgg taatcgtgcg agagggcgca gggacttcct ttgtcccaaa tctggcggag
     1441 ccgaaatctg ggaggcgccg ccgcaccccc tctagcgggc gcgggcgaag cggtgcggcg
     1501 ccggcaggaa ggaaatgggc ggggagggcc ttcgtgcgtc gccgcgccgc cgtccccttc
     1561 tccatctcca gcctcggggc tgccgcaggg ggacggctgc cttcgggggg gacggggcag
     1621 ggcggggttc ggcttctggc gtgtgaccgg cggggtttat atcttccctt ctctgttcct
     1681 ccgcagcccc caagctacca tggcagatcc agccgaatgc agcatcaaag tgatgtgccg
     1741 gttccggccc ctcaacgaag ctgagatcct ccgcggggac aaattcatcc ccaaattcaa
     1801 aggcgaggag acggtggtga tcgggcaagg gaagccgtat gtctttgacc gagttctgcc
     1861 gcccaacaca acccaggagc aggtctacaa tgcctgtgcg aagcagattg tcaaagatgt
     1921 cctcgagggt tacaatggga caatttttgc atacgggcag acttcatcag gaaaaactca
     1981 taccatggag gggaagctgc acgatcctca gcttatgggt atcataccca ggatcgcaca
     2041 cgatattttc gatcacatct attccatgga tgagaacttg gagtttcata tcaaggtttc
     2101 ctattttgag atctacttgg acaaaataag ggacttgctt gacgtgtcca agaccaactt
     2161 ggctgttcat gaagacaaaa acagagtccc ctatgtaaag gggtgcaccg agaggtttgt
     2221 gtcaagcccg gaggaggtca tggatgtgat cgatgagggc aaagcaaacc gacatgtggc
     2281 tgtgacaaac atgaacgaac acagctccag gagtcacagt atcttcctga ttaacattaa
     2341 acaagagaat gtggagactg aaaaaaaact cagcgggaag ctgtatttgg ttgatttggc
     2401 tgggagtgaa aaggtcagca aaactggtgc cgagggagct gttcttgatg aagctaaaaa
     2461 tatcaacaag tctttgtctg ctcttggaaa tgtgatctct gccttggcag aagggacaaa
     2521 aacacatgtg ccataccggg acagcaagat gactcggatt ctccaggact ctctgggtgg
     2581 gaactgcagg accaccattg tcatttgctg ttctccttca gtcttcaatg aggctgagac
     2641 caagtccacg ctgatgtttg gacagagagc aaagaccatc aagaatacag tctctgtgaa
     2701 cttggaacta acagcagaag aatggaagaa gaaatatgaa aaagagaaag agaagaataa
     2761 ggccctgaag agtgtcatcc agcatctgga ggtggagctg aacaggtgga ggaacgggga
     2821 agctgtgcct gaggatgaac agatcagtgc caaggaccag aagaacctgg agccctgcga
     2881 caacacaccc atcatagaca acatcacacc tgtggttgac ggcatctctg ctgagaaaga
     2941 gaagtatgac gaggaaatca ccagtctgta ccgacagctg gatgacaagg acgatgaaat
     3001 taaccagcag agccagctgg ctgaaaagct gaagcaacag atgttggatc aggacgagct
     3061 tctagcttcc accagaaggg actacgagaa aatccaggag gagctgaccc gcctccagat
     3121 tgaaaacgag gcggctaaag acgaggtgaa ggaggtcctc caggccctgg aggagctggc
     3181 tgtcaattac gaccagaagt cacaggaagt ggaggacaag acccgggcca acgagcaact
     3241 gaccgatgag ctggcccaga aaacgacgac actgacaacc acccagcggg agctgagtca
     3301 gctacaggag cttagcaacc accagaagaa gagggccacg gagatcctga acctgcttct
     3361 caaggatctg ggcgagcttg gcgcgcctag gcctaccatg gtgagcaagg gcgaggaggt
     3421 catcaaagag ttcatgcgct tcaaggtgcg catggagggc tccatgaacg gccacgagtt
     3481 cgagatcgag ggcgagggcg agggccgccc ctacgagggc acccagaccg ccaagctgaa
     3541 ggtgaccaag ggcggccccc tgcccttcgc ctgggacatc ctgtcccccc agttcatgta
     3601 cggctccaag gcgtacgtga agcaccccgc cgacatcccc gattacaaga agctgtcctt
     3661 ccccgagggc ttcaagtggg agcgcgtgat gaacttcgag gacggcggtc tggtgaccgt
     3721 gacccaggac tcctccctgc aggacggcac gctgatctac aaggtgaaga tgcgcggcac
     3781 caacttcccc cccgacggcc ccgtaatgca gaagaagacc atgggctggg aggcctccac
     3841 cgagcgcctg tacccccgcg acggcgtgct gaagggcgag atccaccagg ccctgaagct
     3901 gaaggacggc ggccactacc tggtggagtt caagaccatc tacatggcca agaagcccgt
     3961 gcaactgccc ggctactact acgtggacac caagctggac atcacctccc acaacgagga
     4021 ctacaccatc gtggaacagt acgagcgctc cgagggccgc caccacctgt tcctggggca
     4081 tggcaccggc agcaccggca gcggcagctc cggcaccgcc tcctccgagg acaacaacat
     4141 ggccgtcatc aaagagttca tgcgcttcaa ggtgcgcatg gagggctcca tgaacggcca
     4201 cgagttcgag atcgagggcg agggcgaggg ccgcccctac gagggcaccc agaccgccaa
     4261 gctgaaggtg accaagggcg gccccctgcc cttcgcctgg gacatcctgt ccccccagtt
     4321 catgtacggc tccaaggcgt acgtgaagca ccccgccgac atccccgatt acaagaagct
     4381 gtccttcccc gagggcttca agtgggagcg cgtgatgaac ttcgaggacg gcggtctggt
     4441 gaccgtgacc caggactcct ccctgcagga cggcacgctg atctacaagg tgaagatgcg
     4501 cggcaccaac ttcccccccg acggccccgt aatgcagaag aagaccatgg gctgggaggc
     4561 ctccaccgag cgcctgtacc cccgcgacgg cgtgctgaag ggcgagatcc accaggccct
     4621 gaagctgaag gacggcggcc gctacctggt ggagttcaag accatctaca tggccaagaa
     4681 gcccgtgcaa ctgcccggct actactacgt ggacaccaag ctggacatca cctcccacaa
     4741 cgaggactac accatcgtgg aacagtacga gcgctccgag ggccgccacc acctgttcct
     4801 gtacggcatg gacgagctgt acaagcggac cgtctctaga ggagtgcagg tggaaaccat
     4861 ctccccagga gacgggcgca ccttccccaa gcgcggccag acctgcgtgg tgcactacac
     4921 cgggatgctt gaagatggaa agaaatttga ttcctcccgg gacagaaaca agccctttaa
     4981 gtttatgcta ggcaagcagg aggtgatccg aggctgggaa gaaggggttg cccagatgag
     5041 tgtgggtcag agagccaaac tgactatatc tccagattat gcctatggtg ccactgggca
     5101 cccaggcatc atcccaccac atgccactct cgtcttcgat gtggagcttc taaaactgga
     5161 aactagttat tgaggatcat aatcagccat atcacatctg tagaggtttt acttgcttta
     5221 aaaaacctcc cacacctccc cctgaacctg aaacataaaa tgaatgcaat tgttgttgtt
     5281 aacttgttta ttgcagctta taatggttac aaataaagca atagcatcac aaatttcaca
     5341 aataaagcat ttttttcact gcattctagt tgtggtttgt ccaaactcat caatgtatct
     5401 tatcatgtct ggatccccgg gtaccgagct cgaattcgta atcatggtca tagctgtttc
     5461 ctgtgtgaaa ttgttatccg ctcacaattc cacacaacat acgagccgga agcataaagt
     5521 gtaaagcctg gggtgcctaa tgagtgagct aactcacatt aattgcgttg cgctcactgc
     5581 ccgctttcca gtcgggaaac ctgtcgtgcc agctgcatta atgaatcggc caacgcgcgg
     5641 ggagaggcgg tttgcgtatt gggcgctctt ccgcttcctc gctcactgac tcgctgcgct
     5701 cggtcgttcg gctgcggcga gcggtatcag ctcactcaaa ggcggtaata cggttatcca
     5761 cagaatcagg ggataacgca ggaaagaaca tgtgagcaaa aggccagcaa aaggccagga
     5821 accgtaaaaa ggccgcgttg ctggcgtttt tccataggct ccgcccccct gacgagcatc
     5881 acaaaaatcg acgctcaagt cagaggtggc gaaacccgac aggactataa agataccagg
     5941 cgtttccccc tggaagctcc ctcgtgcgct ctcctgttcc gaccctgccg cttaccggat
     6001 acctgtccgc ctttctccct tcgggaagcg tggcgctttc tcatagctca cgctgtaggt
     6061 atctcagttc ggtgtaggtc gttcgctcca agctgggctg tgtgcacgaa ccccccgttc
     6121 agcccgaccg ctgcgcctta tccggtaact atcgtcttga gtccaacccg gtaagacacg
     6181 acttatcgcc actggcagca gccactggta acaggattag cagagcgagg tatgtaggcg
     6241 gtgctacaga gttcttgaag tggtggccta actacggcta cactagaagg acagtatttg
     6301 gtatctgcgc tctgctgaag ccagttacct tcggaaaaag agttggtagc tcttgatccg
     6361 gcaaacaaac caccgctggt agcggtggtt tttttgtttg caagcagcag attacgcgca
     6421 gaaaaaaagg atctcaagaa gatcctttga tcttttctac ggggtctgac gctcagtgga
     6481 acgaaaactc acgttaaggg attttggtca tgagattatc aaaaaggatc ttcacctaga
     6541 tccttttaaa ttaaaaatga agttttaaat caatctaaag tatatatgag taaacttggt
     6601 ctgacagtta ccaatgctta atcagtgagg cacctatctc agcgatctgt ctatttcgtt
     6661 catccatagt tgcctgactc cccgtcgtgt agataactac gatacgggag ggcttaccat
     6721 ctggccccag tgctgcaatg ataccgcgag acccacgctc accggctcca gatttatcag
     6781 caataaacca gccagccgga agggccgagc gcagaagtgg tcctgcaact ttatccgcct
     6841 ccatccagtc tattaattgt tgccgggaag ctagagtaag tagttcgcca gttaatagtt
     6901 tgcgcaacgt tgttgccatt gctacaggca tcgtggtgtc acgctcgtcg tttggtatgg
     6961 cttcattcag ctccggttcc caacgatcaa ggcgagttac atgatccccc atgttgtgca
     7021 aaaaagcggt tagctccttc ggtcctccga tcgttgtcag aagtaagttg gccgcagtgt
     7081 tatcactcat ggttatggca gcactgcata attctcttac tgtcatgcca tccgtaagat
     7141 gcttttctgt gactggtgag tactcaacca agtcattctg agaatagtgt atgcggcgac
     7201 cgagttgctc ttgcccggcg tcaatacggg ataataccgc gccacatagc agaactttaa
     7261 aagtgctcat cattggaaaa cgttcttcgg ggcgaaaact ctcaaggatc ttaccgctgt
     7321 tgagatccag ttcgatgtaa cccactcgtg cacccaactg atcttcagca tcttttactt
     7381 tcaccagcgt ttctgggtga gcaaaaacag gaaggcaaaa tgccgcaaaa aagggaataa
     7441 gggcgacacg gaaatgttga atactcatac tcttcctttt tcaatattat tgaagcattt
     7501 atcagggtta ttgtctcatg agcggataca tatttgaatg tatttagaaa aataaacaaa
     7561 taggggttcc gcgcacattt ccccgaaaag tgccacctga cgtctaagaa accattatta
     7621 tcatgacatt aacctataaa aataggcgta tcacgaggcc ctttcgtctc gcgcgtttcg
     7681 gtgatgacgg tgaaaacctc tgacacatgc agctcccgga gacggtcaca gcttgtctgt
     7741 aagcggatgc cgggagcaga caagcccgtc agggcgcgtc agcgggtgtt ggcgggtgtc
     7801 ggggttggct taactatgcg gcatcagagc agattgtact gagagtgcac catatgcggt
     7861 gtgaaatacc gcacagatgc gtaaggagaa aataccgcat caggcgccat tcgccattca
     7921 ggctgcgcaa ctgttgggaa gggcgatcgg tgcgggcctc ttcgctatta cgccagctgg
     7981 cgaaaggggg atgtgctgca aggcgattaa gttgggtaac gccagggttt tcccagtcac
     8041 gacgttgtaa aacgacggcc agtgccaagt tgggatcttt gcattggccc acggctctca
     8101 ggatggggat gctccccttc agcacccggt tccccttgga aactgatggt cctggctctg
     8161 tggcatggca gtggcactgt gaggagcccc taccagcagc acacagtggg tttggcactg
     8221 ccacgctccg gatgccgcgc tctgatccaa ccccataatc aagggaaccc gaattgcccc
     8281 atcattgccc ccaccacccc catcctgccg ggccctcaca ccccacgctg ccttgtggtg
     8341 acattcccca gcccaaaccc acggcttcat ggctaccgcg gggcatttcc cattgccgcc
     8401 ccattatcag ctctgcacac ctcccgctgt acccatgcct cgtggctgcc cttctttgac
     8461 gtataatctt ctaattaata cccggccttg tcaaagtgga gcacaaacgt taattaattc
     8521 cccagcaggc aggtaattaa cagtgtgact ccctttttgc tgcgagtggg gctgatacag
     8581 agagatgtgg cactatggag cccacggggt cctggcactg ggtgcccacg gaggtcccca
     8641 tgtgctgcag tgtcaccgcc tccgaggtga cagtattgtc cctgcggtgt ccctgcagct
     8701 cagctctgtc cacagggcca cctccagttt ggaggggaca caatgcagcc ccgatgcaac
     8761 ccatcctcgc agcatcccag ggacaaagac cccactgcaa gaccgcacac agggctgggt
     8821 cccgctcccc taatatctac agtgcttttg catggcccct taatcaatgc agttaatcag
     8881 catgcgctca tgcaccgctc tggagctgca aagcccctcg cagcgctgct caccaacacc
     8941 gcgcaccgcc ccggcccagc ctgcagcacg cgctgcaaac aggaaagaaa caaaatattg
     9001 cccaaatgta ggcaaaggca ttcggctgcc ttgacctccg ccgggccggg ccctgcctga
     9061 ctcagctcct tactcagcgc tcgcttcctc cctccggctg ccaccgccgc agcgcacacc
     9121 ctgacaaaga gtggccctta acgggctctg aggtgcaccc agcagtgcac tcagcagtcc
     9181 aagggccggc ctggaggttt gcaccgctac gtgctgacat tagcattgaa cttggccctg
     9241 ggtagtgctg caggccgggc ggggtgggtg tagagagtgc agcgcgcgtt gcacccggtg
     9301 ccccttcccc tcccttgcat cccagcaggc tgcaccccag caccaggccc gtgcatgcat
     9361 gctcctggtg ttattgcagc ctggtgcatg catgcgtctt agtggtgcag cgctgtgcat
     9421 gcatcctcct tggtgtgtag cagc
//
