LOCUS       sequence-107632-        1199 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  mCherry fused between Cry2 and GRK3 ct to study cell migration.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1199)
  AUTHORS   O'Neill PR, Gautam N
  TITLE     Subcellular optogenetic inhibition of G proteins generates signaling
            gradients and cell migration.
  JOURNAL   Mol Biol Cell. 2014 Aug 1;25(15):2305-14. doi: 
            10.1091/mbc.E14-04-0870. Epub 2014 Jun 11.
  PUBMED    24920824
REFERENCE   2  (bases 1 to 1199)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1091/mbc.E14-04-0870"; journalName: "Mol Biol Cell"; date: 
            "2014-08-1- 1"; volume: "25"; issue: "15"; pages: "2305-14"
COMMENT     Sequence Label: Cry2 mCherry GRK3 ct in pcDNA3.1
FEATURES             Location/Qualifiers
     source          1..1199
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     complement(512..529)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    518..742
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     primer_bind     complement(875..894)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     gap             1100..1110
                     /estimated_length=11
ORIGIN
        1 ccgnnnnggg cgcactccac cggcggcatg gacgagctgt acaagggtac catgggccac
       61 gaagaagatt acgccctggg aagagactgc atcgtgcacg ggtacatgct gaagctgggg
      121 aaccctttcc tgacccagtg gcagcgccgc tatttttacc tctttccgaa cagacttgag
      181 tggagaggag aaggcgagtc gcgacaaagt ttactgacaa tggaacagat tgtgtccgtg
      241 gaagaaactc agattaaaga caaaaagtgc attttgttga gaataaaagg agggaagcag
      301 ttcgttttgc agtgtgagag tgacccagag tttgtgcagt ggaagaaaga gctgacggag
      361 acattcatgg aggcccagcg gctgctacgg cgagccccca agttcctcaa caaatcccgc
      421 tcagccgtcg tggaactctc aaagcctccc ctctgccata ggaacagcaa tggcctctag
      481 tctagagggc ccgtttaaac ccgctgatca gcctcgactg tgccttctag ttgccagcca
      541 tctgttgttt gcccctcccc cgtgccttcc ttgaccctgg aaggtgccac tcccactgtc
      601 ctttcctaat aaaatgagga aattgcatcg cattgtctga gtaggtgtca ttctattctg
      661 gggggtgggg tggggcagga cagcaagggg gaggattggg aagacaatag caggcatgct
      721 ggggatgcgg tgggctctat ggcttctgag gcggaaagaa ccagctgggg ctctaggggg
      781 tatccccacg cgccctgtag cggcgcatta agcgcggcgg gtgtggtggt tacgcgcagc
      841 gtgaccgcta cacttgccag cgccctagcg cccgctcctt tcgctttctt cccttccttt
      901 ctcgcacgtt cggcggcttt ccccgtcaag ctctaaatcg ggggctccct ttagggntcc
      961 gatttagtgc tttacggcac ctcgacccca aaaacttgat agggtgatgg gtcacgtagn
     1021 gggncatcgc cctgatagac ggttttcgcc cttttgacgt tnngtcacgt tctttnnagn
     1081 ggactcctnn ncaactggan nnnnnnnnnn aatcngntaa tccttgaatt nnnngattgc
     1141 cgattcgnnn ttgnnnannt gaggctgatn nnnnntnnnc cgnananntc ngtggaang
//
