LOCUS       sequence-107633-         627 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Wild-type OmpA (N-terminal domain) with an HA tag in the 4th 
            external loop.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 627)
  AUTHORS   Stapleton JA, Whitehead TA, Nanda V
  TITLE     Computational redesign of the lipid-facing surface of the outer 
            membrane protein OmpA.
  JOURNAL   Proc Natl Acad Sci U S A. 2015 Jul 21. pii: 201501836.
  PUBMED    26199411
REFERENCE   2  (bases 1 to 627)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Proc Natl 
            Acad Sci U S A. 2015 Jul 21. pii: 201501836."
COMMENT     Sequence Label: pJexpress414_OmpA171_WT
FEATURES             Location/Qualifiers
     source          1..627
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     sig_peptide     1..63
                     /codon_start=1
                     /product="signal peptide from the E. coli outer membrane 
                     protein OmpA"
                     /label=OmpA signal peptide
                     /translation="MKKTAIAIAVALAGFATVAQA"
     CDS             511..537
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     CDS             589..606
                     /codon_start=1
                     /product="tetracysteine peptide that binds biarsenical 
                     labeling reagents"
                     /label=tetracysteine tag
                     /translation="CCPGCC"
     CDS             607..624
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
ORIGIN
        1 atgaaaaaga ccgcaatcgc catcgcagtg gcactggctg gtttcgctac cgtagcgcag
       61 gccgcgccga acgacaacac ctggtatact ggtgctaaac tgggctggtc ccagtaccac
      121 gacactggtt tcatcaataa taatggcccg acccacgaga accagctggg cgctggtgct
      181 tttggtggtt accaggtgaa cccgtacgtt ggctttgaaa tgggttacga ctggctcggt
      241 cgtatgccgt acaaaggcag cgttgaaaac ggtgcataca aggctcaggg cgttcaactg
      301 accgctaaac tgggttaccc gatcaccgac gacctggata tctatactcg tctgggtggc
      361 atggtatggc gtgcagatac taaatccaac gtttatggta aaaaccacga taccggcgtt
      421 tctccggtct tcgctggcgg tgttgagtac gcgatcactc cggagatcgc tacccgtctg
      481 gaataccagt ggaccaacaa catcggtgac tacccatacg atgttccaga ttacgcgact
      541 cgtccggata acggcatgct gagcctgggt gtttcctacc gtttcggttg ttgccctggt
      601 tgctgtcacc atcaccacca tcactaa
//
