LOCUS       sequence-107642-         749 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  PCR template for the insertion of eGFP by en passant (two-step 
            Red-mediated) mutagenesis.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 749)
  AUTHORS   Tischer BK, von Einem J, Kaufer B, Osterrieder N
  TITLE     Two-step red-mediated recombination for versatile high-efficiency 
            markerless DNA manipulation in Escherichia coli.
  JOURNAL   Biotechniques. 2006 Feb;40(2):191-7.
  PUBMED    16526409
REFERENCE   2  (bases 1 to 749)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Biotechniques."; date: "2006-02"; volume: "40(2)"; pages: "191-7"
COMMENT     Sequence Label: pEP-EGFPin
FEATURES             Location/Qualifiers
     source          1..749
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     complement(32..53)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(287..306)
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
     primer_bind     complement(371..390)
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
ORIGIN
        1 cgaggagctg ttcaccgggg tggtgcccat cctggtcgag ctggacggcg acgtaaacgg
       61 ccacaagttc agcgtgtccg gcgagggcga gggcgatgcc acctacggca agctgaccct
      121 gaagttcatc tgcaccaccg gcaagctgcc actagctagg gataacaggg taatcgattt
      181 attcaacaaa gccacgttgt gtctcaaaat ctctgatgtt acattgcaca agataaaaat
      241 atatcatcat gaacaataaa actgtctgct tacataaaca gtaatacaag gggtgttatg
      301 agccatattc aacgggaaac gtcttgctcg aggccgcgat taaattccaa catggatgct
      361 gatttatatg ggtataaatg ggctcgcgat aatgtcgggc aatcaggtgc gacaatctat
      421 cgattgtatg ggaagcccga tgcgccagag ttgtttctga aacatggcaa aggtagcgtt
      481 gccaatgatg ttacagatga gatggtcaga ctaaactggc tgacggaatt tatgcctctt
      541 ccgaccatca agcattttat ccgtactcct gatgatgcat ggttactcac cactgcgatc
      601 cccgggaaaa cagcattcca ggtattagaa gaatatcctg attcaggtga aaatattgtt
      661 gatgcgctgg cagtgttcct gcgccggttg cattcgattc ctgtttgtaa ttgtcctttt
      721 aacagcgatc gcgtatttcg tctcgctca
//
