LOCUS       sequence-107647-         679 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  sgRNA + 2x MS2(wt+f6) with MCP-VP64 effector for mammalian cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 679)
  AUTHORS   Zalatan JG, Lee ME, Almeida R, Gilbert LA, Whitehead EH, La Russa M,
            Tsai JC, Weissman JS, Dueber JE, Qi LS, Lim WA
  TITLE     Engineering Complex Synthetic Transcriptional Programs with CRISPR 
            RNA Scaffolds.
  JOURNAL   Cell. 2014 Dec 18. pii: S0092-8674(14)01570-0. doi: 
            10.1016/j.cell.2014.11.052.
  PUBMED    25533786
REFERENCE   2  (bases 1 to 679)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell. 2014 
            Dec 18. pii: S0092-8674(14)01570-0. doi: 
            10.1016/j.cell.2014.11.052."
COMMENT     Sequence Label: pJZC34
FEATURES             Location/Qualifiers
     source          1..679
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    complement(260..293)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     primer_bind     complement(402..422)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
ORIGIN
        1 gaggacggcg ccctgaaggg cgagatcaag cagaggctga agctgaagga cggcggccac
       61 tacgacgctg aggtcaagac cacctacaag gccaagaagc ccgtgcagct gcccggcgcc
      121 tacaacgtca acatcaagtt ggacatcacc tcccacaacg aggactacac catcgtggaa
      181 cagtacgaac gcgccgaggg ccgccactcc accggcggca tggacgagct gtacaagtaa
      241 gaattcgtcg agggacctaa taacttcgta tagcatacat tatacgaagt tatacatgtt
      301 taagggttcc ggttccacta ggtacaattc gatatcaagc ttatcgataa tcaacctctg
      361 gattacaaaa tttgtgaaag attgactggt attcttaact atgttgctcc ttttacgcta
      421 tgtggatacg ctgctttaat gcctttgtat catgctattg cttcccgtat ggctttcatt
      481 ttctcctcct tgtataaatc ctggttgctg tctctttatg aggagttgtg gcccgttgtc
      541 aggcaacgtg gcgtggtgtg cactgtgttt gctgacgcaa cccccactgg ttggggcatt
      601 gccaccacct gtcagctcct ttccgggact ttcgctttcc ccctccctat tgccacggcg
      661 gaactcatcg ccgcctgcc
//
