LOCUS       sequence-107649-         694 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  sgRNA + 2x MS2(wt+f6) with MCP-VP64 effector for mammalian cells, 
            marked by BFP.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 694)
  AUTHORS   Zalatan JG, Lee ME, Almeida R, Gilbert LA, Whitehead EH, La Russa M,
            Tsai JC, Weissman JS, Dueber JE, Qi LS, Lim WA
  TITLE     Engineering Complex Synthetic Transcriptional Programs with CRISPR 
            RNA Scaffolds.
  JOURNAL   Cell. 2014 Dec 18. pii: S0092-8674(14)01570-0. doi: 
            10.1016/j.cell.2014.11.052.
  PUBMED    25533786
REFERENCE   2  (bases 1 to 694)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell. 2014 
            Dec 18. pii: S0092-8674(14)01570-0. doi: 
            10.1016/j.cell.2014.11.052."
COMMENT     Sequence Label: pJZC116
FEATURES             Location/Qualifiers
     source          1..694
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(61..210)
                     /codon_start=1
                     /product="tetrameric repeat of the minimal activation 
                     domain of herpes simplex virus VP16 (Beerli et al., 1998)"
                     /label=VP64
                     /translation="DALDDFDLDMLGSDALDDFDLDMLGSDALDDFDLDMLGSDALDDF
                     DLDML"
     CDS             complement(583..603)
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     promoter        complement(640..691)
                     /label=CMVd2 promoter
                     /note="truncated human cytomegalovirus (CMV) immediate 
                     early promoter that yields moderately reduced expression 
                     relative to the full-length promoter"
     primer_bind     complement(669..689)
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
ORIGIN
        1 ctcctccacg tctccagcct gcttcagcag gctgaagtta gtagcgccgc tgccgttgat
       61 gagcatgtcc agatcgaagt catccagggc gtcggagccc agcatgtcga gatcgaagtc
      121 gtccagagcg tcactcccca gcatgtcaag gtcgaagtca tcgagagcgt cggatcccag
      181 catatcgagg tcaaaatcgt cgagagcatc ggctcgtccg gagccgtaga tgccggagtt
      241 tgctgcgatt gctgagggaa tcgggtttcc atcttttagg agaccttgca ttgccttaac
      301 aataagctcg cagtcggaat tcgtggcgaa aattggaatg gttagttcca tatttaagta
      361 cgaacgccag gcgcctttag gcacctcgac tttgatggtg tatttgcgat tctgcgcaga
      421 gctctgacga acgctacagg ttactttgta agcctgtgaa cgcgagttag agctgatcca
      481 ttcagcgatc ccgttagcga agttgcttgg ggcgacagtc acgtcgccag ttccgccatt
      541 gtcgacgaga acgaactgag taaagttaga agccatacta cccacttttc ttttcttttt
      601 gggcatggtg ggctagcgga tctgacggtt cactaaacca gctctgctta tatagacctc
      661 ccaccgtaca cgcctaccgc ccatttgcgt caat
//
