LOCUS       sequence-10765-e        2295 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 2295)
  AUTHORS   Johannessen CM, Boehm JS, Kim SY, Thomas SR, Wardwell L, Johnson LA,
            Emery CM, Stransky N, Cogdill AP, Barretina J, Caponigro G, 
            Hieronymus H, Murray RR, Salehi-Ashtiani K, Hill DE, Vidal M, Zhao 
            JJ, Yang X, Alkan O, Kim S, Harris JL, Wilson CJ, Myer VE, Finan PM,
            Root DE, Roberts TM, Golub T, Flaherty KT, Dummer R, Weber BL, 
            Sellers WR, Schlegel R, Wargo JA, Hahn WC, Garraway LA
  TITLE     COT drives resistance to RAF inhibition through MAP kinase pathway 
            reactivation.
  JOURNAL   Nature. 2010 Dec 16;468(7326):968-72. doi: 10.1038/nature09627
  PUBMED    21107320
REFERENCE   2  (bases 1 to 2295)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10"; journalName: 
            "Nature"; date: "2010-12-16- 16"; volume: "468"; issue: "7326"; 
            pages: "968-72"
COMMENT     Sequence Label: pDONR223-FASTKD5
FEATURES             Location/Qualifiers
     source          1..2295
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     RBS             1526..1534
                     /label=Shine-Dalgarno sequence
                     /note="full consensus sequence for ribosome-binding sites 
                     upstream of start codons in E. coli; complementary to a 
                     region in the 3' end of the 16S rRNA (Chen et al., 1994)"
ORIGIN
        1 atggcagcta ctctcaagtc attaaaactt gtaagatacc gagcattttg cagtccttct
       61 gcctttggtg cagtccgaag tgtgtcatac tggaatgtga gcagcacaca gcatggggga
      121 caggaccctc cagaacacat tagcctctgc cattctgcca aaaaagttaa gaacatatgt
      181 agcaccttct cttctcggag aatcctgaca accagcagtg cccacccagg tttggaattc
      241 agcaagactt cttcctctaa ggccagtaca ttgcagctgg gctcacccag ggccacagga
      301 gttgatgaag aggacgtaga agtgtttgat tcctttgaaa acatgcgagt tttcctacag
      361 ctaagaccag aataccgtgt tcacagctat aatgcatctg agacttctca gctcctgtct
      421 gtttcagaag gtgaactaat tttgcacaaa gtcagagtta atcaaaataa tctccaggct
      481 caagtcattg ttgattattt gtgtaagctg agctctttgc ctgcagagca gcatcctgtc
      541 ttgctgggca gtaccagctt tgctctgctc tgccagctga gtgtgaagaa gatacagctc
      601 tttgataccc aagatctgat caatgttttg aaagcttttg tcattttagg aatccctcac
      661 tcccattcaa tgctagatgt gtatgagacc aagtgttgcc atcaggtatg ggagatgaat
      721 atggatcagc tccttttggt ggctgatctc tggaggtact taggccgcaa agtacctagg
      781 tttttaaaca ttttttctag ttatcttaat ttgcactgga aggatctatc cttgtctcag
      841 ctagttcact taatttatgt tataggtgaa aatcgtcagg tatcccagga cctaatgcaa
      901 aaattggaat cattgatcct taaatatata gatttgatca atttggagga ggttggtacc
      961 atctgtttgg ggttctttaa atcaagtact aatctctctg aatttgtcat gcggaaaatt
     1021 ggagacttgg cttgtgctaa cattcagcat ctgagtagtc gctccttagt gaatattgtt
     1081 aaaatgttcc gtttcactca cgtggatcac atcaatttca tgaagcagat tggagagata
     1141 gctcctcagc gaattccttc cctgggagtt caaggtgtca tgcacctgac tctttactgc
     1201 tcggccttac gcttcctgaa tgaaggagta atgaatgcag tggctgcgtc tttgcctcct
     1261 agagtggcac actgtcgaag taaagatgtt gccaagattc tgtggtcatt tggaactctg
     1321 aattataagc cacccaatgc agaagaattt tactccagcc tgataagtga gattcacaga
     1381 aagatgcctg aattcaacca gtacccagaa cacctgccca cctgcctgct gggcctggca
     1441 tttttggagt actttcctgt agagttaatt gatttcgctc tcagtccagg gtttgtcagg
     1501 ttagctcagg agagaactaa gtttgacctc cttaaggaac tatataccct cgatggtaca
     1561 gttggcattg agtgtccaga ttacagaggc aatcgtctta gtactcacct tcagcaagag
     1621 gggtctgaat tgctgtggta tttagcagag aaggatatga attcaaagcc tgaattctta
     1681 gaaactgtct ttttactgga gaccatgctg ggggggcccc agtacgtcaa gcaccatatg
     1741 attttgcctc atacccgatc ttctgactta gaggtccagc ttgatgttaa cctgaagcca
     1801 ttaccattta atagagaagc cacgccggct gaaaatgtag ccaaattaag gcttgagcat
     1861 gtgggagtca gccttacaga tgatttgatg aataagttac taaaagggaa agcaagagga
     1921 catttccagg gcaaaactga gtcagagcct gggcagcagc ccatggagtt ggagaataag
     1981 gcagctgtac ctctgggggg cttcctttgc aatgtagcag ataaatcagg ggccatggag
     2041 atggctggcc tgtgccccgc agcctgcatg cagaccccaa gaatgaagct ggctgttcag
     2101 ttcacaaaca ggaaccagta ttgctatggc tccagggatc tccttggact gcacaatatg
     2161 aagaggcggc agctggctcg gcttggctac cgtgtggtag agttatccta ctgggaatgg
     2221 ctcccactac tgaaacgaac tcgcttagaa aagttggcgt ttcttcatga gaaagtattc
     2281 acctctgctc tctga
//
