LOCUS       sequence-107657-         571 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  adenoviral shuttle vector containing N-terminal Flag tag.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 571)
  AUTHORS   Lingfang Zeng, Qingbo Xu
  TITLE     pShuttle-Flag-HA vector
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 571)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: pShuttle-Flag-HA
FEATURES             Location/Qualifiers
     source          1..571
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     22..41
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        22..40
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             63..86
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     CDS             159..185
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     CDS             186..212
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     polyA_signal    234..315
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(271..290)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     325..344
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(481..498)
                     /label=L4440
                     /note="L4440 vector, forward primer"
ORIGIN
        1 tgcttactgg cttatcgaaa ttaatacgac tcactatagg gagacccaag ctggctagca
       61 tggactacaa ggacgacgat gacaaggaat tcagatctgg taccacgcgt atcgataagc
      121 ttgcatgcct gcaggtcgac tctagaggat ccctggatta tccgtatgat gttcctgatt
      181 atgcataccc ctacgacgtg ccagactacg cgcttaagta agtaagtgac tagaacttgt
      241 ttattgcagc ttataatggt tacaaataaa gcaatagcat cacaaatttc acaaataaag
      301 catttttttc actgcattct agttgtggtt tgtccaaact catctgcaga tctgaattca
      361 tctatgtcgg gtgcggagaa agaggtaatg aaatggcatt atgggtatta tgggtctgca
      421 ttaatgaatc ggccaacgcg cggggagagg cggtttgcgt attgggcgct cttccgcttc
      481 ctcgctcact gactcgctgc gctcggtcgt tcggctgcgg cgagcggtat cagctcactc
      541 aaaggcggta atacggttat ccacagaatc a
//
