LOCUS       sequence-107659-         498 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Adenoviral shuttle vector for XBP1u expression.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 498)
  AUTHORS   Zeng L, Zampetaki A, Margariti A, Pepe AE, Alam S, Martin D, Xiao Q,
            Wang W, Jin ZG, Cockerill G, Mori K, Li YS, Hu Y, Chien S, Xu Q
  TITLE     Sustained activation of XBP1 splicing leads to endothelial apoptosis
            and atherosclerosis development in response to disturbed flow.
  JOURNAL   Proc Natl Acad Sci U S A. 2009 May 19;106(20):8326-31. doi: 
            10.1073/pnas.0903197106. Epub 2009 May 1.
  PUBMED    19416856
REFERENCE   2  (bases 1 to 498)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1073/pnas.0903197106"; journalName: "Proc Natl Acad Sci U S A"; 
            date: "2009-05-19- 19"; volume: "106"; issue: "20"; pages: "8326-31"
COMMENT     Sequence Label: pShuttle-Flag-XBP1u
FEATURES             Location/Qualifiers
     source          1..498
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     24..43
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        24..42
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             65..88
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
ORIGIN
        1 actgcttact ggcttatcga aattaatacg actcactata gggagaccca agctggctag
       61 catggactac aaggacgacg atgacaagga attcagatct ggtaccctgg tggtggtggc
      121 agccgcgccg aacccggccg acgggacccc taaagttctg cttctgtcgg ggcagcccgc
      181 ctccgccgcc ggagccccgg ccggccaggc cctgccgctc atggtgccag cccagagagg
      241 ggccagcccg gaggcagcga gcggggggct gccccaggcg cgcaagcgac agcgcctcac
      301 gcacctgagc cccgaggaga aggcgctgag gaggaaactg aaaaacagag tagcagctca
      361 gactgccaga gatcgaaaga aggctcgaat gagtgagctg gaacagcaag tggtagattt
      421 agaagaagag aaccaaaaac ttttgctaga aaatcagctt ttacgagaga aaactcatgg
      481 ccttgtagtt gagaacca
//
