LOCUS       sequence-107664-         681 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  This pUC19 vector contains a tetramethylrosamine (TMR) binding 
            riboswitch, controlling the translation initaition rate of 
            luciferase gene. .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 681)
  AUTHORS   Espah Borujeni A, Mishler DM, Wang J, Huso W, Salis HM
  TITLE     Automated physics-based design of synthetic riboswitches from 
            diverse RNA aptamers.
  JOURNAL   Nucleic Acids Res. 2015 Nov 30. pii: gkv1289.
  PUBMED    26621913
REFERENCE   2  (bases 1 to 681)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nucleic 
            Acids Res. 2015 Nov 30. pii: gkv1289."
COMMENT     Sequence Label: AEB-TMR10pUC19
FEATURES             Location/Qualifiers
     source          1..681
                     /mol_type="genomic DNA"
                     /organism="unspecified"
ORIGIN
        1 tttggacttt ccgcccttct tggcctttat gaggatctct ctgatttttc ttgcgtcgag
       61 ttttccggta agacctttcg gtacttcgtc cacaaacaca actcctccgc gcaacttttt
      121 cgcggttgtt acttgactgg cgacgtaatc cacgatctct ttttccgtca tcgtctttcc
      181 gtgctccaaa acaacaacgg cggcgggaag ttcaccggcg tcatcgtcgg gaagacctgc
      241 cacgcccgcg tcgaagatgt tggggtgttg taacaatatc gattccaatt cagcgggggc
      301 cacctgatat cctttgtatt taattaaaga cttcaagcgg tcaactatga agaagtgttc
      361 gtcttcgtcc cagtaagcta tgtctccaga atgtagccat ccatccttgt caatcaaggc
      421 gttggtcgct tccggattgt ttacataacc ggacataatc ataggtcctc tgacacataa
      481 ttcgcctctc tgattaacgc ccagcgtttt cccggtatcc agatccacaa ccttcgcttc
      541 aaaaaatgga acaactttac cgaccgcgcc cggtttatca tccccctcgg gtgtaatcag
      601 aatagctgat gtagtctcag tgagcccata tccttgtcgt atccctggaa gatggaagcg
      661 ttttgcaacc gcttccccga c
//
