LOCUS       sequence-107671-         719 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  This pFTV1 vector contains a dopamine binding riboswitch, 
            controlling the translation initaition rate of luciferase gene. .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 719)
  AUTHORS   Espah Borujeni A, Mishler DM, Wang J, Huso W, Salis HM
  TITLE     Automated physics-based design of synthetic riboswitches from 
            diverse RNA aptamers.
  JOURNAL   Nucleic Acids Res. 2015 Nov 30. pii: gkv1289.
  PUBMED    26621913
REFERENCE   2  (bases 1 to 719)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nucleic 
            Acids Res. 2015 Nov 30. pii: gkv1289."
COMMENT     Sequence Label: AEB-Dopa5
FEATURES             Location/Qualifiers
     source          1..719
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        complement(32..134)
                     /label=cat promoter
                     /note="promoter of the E. coli cat gene encoding 
                     chloramphenicol acetyltransferase"
     promoter        join(192..197,198..213,214..220)
                     /label=tac promoter
                     /note="strong E. coli promoter; hybrid between the trp and 
                     lac UV5 promoters"
     primer_bind     complement(408..426)
                     /label=LucNrev
                     /note="Firefly luciferase, reverse primer"
ORIGIN
        1 cggtggtata tccagtgatt tttttctcca ttttagcttc cttagctcct gaaaatctcg
       61 ataactcaaa aaatacgccc ggtagtgatc ttatttcatt atggtgaaag ttggaacctc
      121 ttacgtgccg atcaacgtct cattttcgcc agatatcctc gagcgcggaa ttccctaggg
      181 gatccgagct gttgacaatt aatcatcggc tcgtataatg tgtgggacgt cgcgacggta
      241 tagcgacccc gtctctgtgt gcgccagaga cactggggca gatatgggcc agcacagaat
      301 gaggccccct agtaacttta cggagggcca gcatggaagc tttcgaagac gccaaaaaca
      361 taaagaaagg cccggcgcca ttctatcctc tagaggatgg aaccgctgga gagcaactgc
      421 ataaggctat gaagagatac gccctggttc ctggaacaat tgcttttaca gatgcacata
      481 tcgaggtgaa catcacgtac gcggaatact tcgaaatgtc cgttcggttg gcagaagcta
      541 tgaaacgata tgggctgaat acaaatcaca gaatcgtcgt atgcagtgaa aactctcttc
      601 aattctttat gccggtgttg ggcgcgttat ttatcggagt tgcagttgcg cccgcgaacg
      661 acatttataa tgaacgtgaa ttgctcaaca gtatgaacat ttcgcagcct accgtagtg
//
