LOCUS       sequence-107684-        8801 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses miR-29b-1 and miR-29a in mammalian cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8801)
  AUTHORS   Chang TC, Yu D, Lee YS, Wentzel EA, Arking DE, West KM, Dang CV, 
            Thomas-Tikhonenko A, Mendell JT
  TITLE     Widespread microRNA repression by Myc contributes to tumorigenesis.
  JOURNAL   Nat Genet. 2008 Jan;40(1):43-50. Epub 2007 Dec 9.
  PUBMED    18066065
REFERENCE   2  (bases 1 to 8801)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Genet."; date: "2008-01"; volume: "40(1)"; pages: "43-50. Epub 2007 
            Dec 9"
COMMENT     Sequence Label: pMSCV-PIG-miR-29b-1/miR-29a
FEATURES             Location/Qualifiers
     source          1..8801
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    579..920
                     /label=MESV Psi
                     /note="packaging signal of murine embryonic stem cell 
                     virus"
     CDS             987..1403
                     /label=gag (truncated)
                     /note="truncated Moloney murine leukemia virus (MMLV) gag 
                     gene lacking the start codon"
     primer_bind     1333..1355
                     /label=pLXSN 5'
                     /note="Murine stem cell virus, forward primer. Also called 
                     MSCV"
     primer_bind     1371..1387
                     /label=pBABE 5'
                     /note="Psi packaging signal, forward primer for pBABE 
                     vectors"
     promoter        2582..3081
                     /label=PGK promoter
                     /note="mouse phosphoglycerate kinase 1 promoter"
     primer_bind     complement(2617..2640)
                     /label=MSCV-rev
                     /note="Murine stem cell virus, reverse primer"
     primer_bind     3014..3033
                     /label=mPGK-F
                     /note="Mouse PGK promoter, forward primer"
     CDS             3102..3701
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(3102..3121)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     3598..3618
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     misc_feature    3762..4328
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     misc_feature    3779..4330
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(3923..3940)
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     primer_bind     4150..4169
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     CDS             join(4342..4344,4345..4347,4348..5061)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             join(4342..4344,4345..4347,4348..5058)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(4387..4408)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(4648..4667)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     4995..5016
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     LTR             5163..5677
                     /label=3' LTR
                     /note="3' long terminal repeat from murine embryonic stem 
                     cell virus"
     primer_bind     complement(5846..5862)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(5846..5862)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(5859..5881)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    5870..5886
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(5894..5900,5901..5918,5919..5924))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    5939..5960
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(6077..6094)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(6248..6836)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(6328..6347)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(7007..7798,7799..7867))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     7630..7649
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(7868..7972)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     8040..8058
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     primer_bind     complement(8096..8118)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     8218..8237
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     8431..8453
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     8581..8600
                     /label=pBRrevBam
                     /note="pBR322 vectors, tet region, downstream of BamHI, 
                     reverse primer"
     LTR             join(8800..8801,1..515)
                     /label=5' LTR
                     /note="5' long terminal repeat from murine embryonic stem 
                     cell virus"
ORIGIN
        1 tgaaagaccc cacctgtagg tttggcaagc tagcttaagt aacgccattt tgcaaggcat
       61 ggaaaataca taactgagaa tagagaagtt cagatcaagg ttaggaacag agagacagca
      121 gaatatgggc caaacaggat atctgtggta agcagttcct gccccggctc agggccaaga
      181 acagatggtc cccagatgcg gtcccgccct cagcagtttc tagagaacca tcagatgttt
      241 ccagggtgcc ccaaggacct gaaatgaccc tgtgccttat ttgaactaac caatcagttc
      301 gcttctcgct tctgttcgcg cgcttctgct ccccgagctc aataaaagag cccacaaccc
      361 ctcactcggc gcgccagtcc tccgatagac tgcgtcgccc gggtacccgt attcccaata
      421 aagcctcttg ctgtttgcat ccgaatcgtg gactcgctga tccttgggag ggtctcctca
      481 gattgattga ctgcccacct cgggggtctt tcatttggag gttccaccga gatttggaga
      541 cccctgccca gggaccaccg acccccccgc cgggaggtaa gctggccagc ggtcgtttcg
      601 tgtctgtctc tgtctttgtg cgtgtttgtg ccggcatcta atgtttgcgc ctgcgtctgt
      661 actagttagc taactagctc tgtatctggc ggacccgtgg tggaactgac gagttctgaa
      721 cacccggccg caaccctggg agacgtccca gggactttgg gggccgtttt tgtggcccga
      781 cctgaggaag ggagtcgatg tggaatccga ccccgtcagg atatgtggtt ctggtaggag
      841 acgagaacct aaaacagttc ccgcctccgt ctgaattttt gctttcggtt tggaaccgaa
      901 gccgcgcgtc ttgtctgctg cagcgctgca gcatcgttct gtgttgtctc tgtctgactg
      961 tgtttctgta tttgtctgaa aattagggcc agactgttac cactccctta agtttgacct
     1021 taggtcactg gaaagatgtc gagcggatcg ctcacaacca gtcggtagat gtcaagaaga
     1081 gacgttgggt taccttctgc tctgcagaat ggccaacctt taacgtcgga tggccgcgag
     1141 acggcacctt taaccgagac ctcatcaccc aggttaagat caaggtcttt tcacctggcc
     1201 cgcatggaca cccagaccag gtcccctaca tcgtgacctg ggaagccttg gcttttgacc
     1261 cccctccctg ggtcaagccc tttgtacacc ctaagcctcc gcctcctctt cctccatccg
     1321 ccccgtctct cccccttgaa cctcctcgtt cgaccccgcc tcgatcctcc ctttatccag
     1381 ccctcactcc ttctctaggc gccggaatta gatctctcga ggcatgctct cccatcaata
     1441 acaaattcag tgacatcagt ttatgaatat atgaaatttg ccaaagctct gtttagacca
     1501 ctgagtaact cacagctagg tttcaacttt tcctttctag gttgtcttgg gtttattgta
     1561 agagagcatt atgaagaaaa aaatagatca taaagcttct tcaggaagct ggtttcatat
     1621 ggtggtttag atttaaatag tgattgtcta gcaccatttg aaatcagtgt tcttggggga
     1681 gaccagctgc gctgcactac caacagcaaa agaagtgaat gggacagctc tgaagtattt
     1741 gaaagcaaca gcaggatggc tgtgagaacc tgcctcacat gtagctgacc ccttcctcac
     1801 ccctgccaac agtggtggca tatatcacaa atggcagtca ggtctctgca ctggcggatc
     1861 caactgtgat cgaaagtttt ccaaaaataa gttgtgtctg tattgaacat gaacagactt
     1921 tcttcttgtc attattctct aacaatactg cataacaatt atttgcatac atttgcattg
     1981 cattaagtat tctaagtaat ctagagacga tttaaagtat acgggaggat gtgtgtaggt
     2041 tgtatgcaaa tactacacca ttttctatca gagacttgag catctgtgga ttttggtatc
     2101 caaggggctt tctggaacca atccctcaag gataccaagg gatgaatgta attgtacagg
     2161 atatcgcatt gttggaattt tatacttctt tgtggaataa acctatagca cttaatagat
     2221 agtacagact cattccattg tgcctgggtt aaagagccca atgtatgctg gatttagtaa
     2281 gatttgggcc ctcccaaccc tcacgacctt ctgtgacccc ttagaggatg actgatttct
     2341 tttggtgttc agagtcaata taattttcta gcaccatctg aaatcggtta taatgattgg
     2401 ggaagagcac catgatgctg actgctgaga ggaaatgtat tggtgaccgt tggggccatg
     2461 gacaagaact aagaaaacaa atgcaaagca ataatgcaaa ggtgattttt cttcttccag
     2521 tttctaagtt gaatttcact gacctgaatt gcatgtggtc tcgaggttaa cgaattctac
     2581 cgggtagggg aggcgctttt cccaaggcag tctggagcat gcgctttagc agccccgctg
     2641 ggcacttggc gctacacaag tggcctctgg cctcgcacac attccacatc caccggtagg
     2701 cgccaaccgg ctccgttctt tggtggcccc ttcgcgccac cttctactcc tcccctagtc
     2761 aggaagttcc cccccgcccc gcagctcgcg tcgtgcagga cgtgacaaat ggaagtagca
     2821 cgtctcacta gtctcgtgca gatggacagc accgctgagc aatggaagcg ggtaggcctt
     2881 tggggcagcg gccaatagca gctttgctcc ttcgctttct gggctcagag gctgggaagg
     2941 ggtgggtccg ggggcgggct caggggcggg ctcaggggcg gggcgggcgc ccgaaggtcc
     3001 tccggaggcc cggcattctg cacgcttcaa aagcgcacgt ctgccgcgct gttctcctct
     3061 tcctcatctc cgggcctttc gacctgcagc ccaagcttac catgaccgag tacaagccca
     3121 cggtgcgcct cgccacccgc gacgacgtcc ccagggccgt acgcaccctc gccgccgcgt
     3181 tcgccgacta ccccgccacg cgccacaccg tcgatccgga ccgccacatc gagcgggtca
     3241 ccgagctgca agaactcttc ctcacgcgcg tcgggctcga catcggcaag gtgtgggtcg
     3301 cggacgacgg cgccgcggtg gcggtctgga ccacgccgga gagcgtcgaa gcgggggcgg
     3361 tgttcgccga gatcggcccg cgcatggccg agttgagcgg ttcccggctg gccgcgcagc
     3421 aacagatgga aggcctcctg gcgccgcacc ggcccaagga gcccgcgtgg ttcctggcca
     3481 ccgtcggcgt ctcgcccgac caccagggca agggtctggg cagcgccgtc gtgctccccg
     3541 gagtggaggc ggccgagcgc gccggggtgc ccgccttcct ggagacctcc gcgccccgca
     3601 acctcccctt ctacgagcgg ctcggcttca ccgtcaccgc cgacgtcgag gtgcccgaag
     3661 gaccgcgcac ctggtgcatg acccgcaagc ccggtgcctg acgcccgccc cacgacccgc
     3721 agcgcccgac cgaaaggagc gcacgacccc atgcatcgaa ttccgccccc cccccctaac
     3781 gttactggcc gaagccgctt ggaataaggc cggtgtgcgt ttgtctatat gttattttcc
     3841 accatattgc cgtcttttgg caatgtgagg gcccggaaac ctggccctgt cttcttgacg
     3901 agcattccta ggggtctttc ccctctcgcc aaaggaatgc aaggtctgtt gaatgtcgtg
     3961 aaggaagcag ttcctctgga agcttcttga agacaaacaa cgtctgtagc gaccctttgc
     4021 aggcagcgga accccccacc tggcgacagg tgcctctgcg gccaaaagcc acgtgtataa
     4081 gatacacctg caaaggcggc acaaccccag tgccacgttg tgagttggat agttgtggaa
     4141 agagtcaaat ggctctcctc aagcgtattc aacaaggggc tgaaggatgc ccagaaggta
     4201 ccccattgta tgggatctga tctggggcct cggtgcacat gctttacatg tgtttagtcg
     4261 aggttaaaaa acgtctaggc cccccgaacc acggggacgt ggttttcctt tgaaaaacac
     4321 gatgataata tggccacaac catggtgagc aagggcgagg agctgttcac cggggtggtg
     4381 cccatcctgg tcgagctgga cggcgacgta aacggccaca agttcagcgt gtccggcgag
     4441 ggcgagggcg atgccaccta cggcaagctg accctgaagt tcatctgcac caccggcaag
     4501 ctgcccgtgc cctggcccac cctcgtgacc accctgacct acggcgtgca gtgcttcagc
     4561 cgctaccccg accacatgaa gcagcacgac ttcttcaagt ccgccatgcc cgaaggctac
     4621 gtccaggagc gcaccatctt cttcaaggac gacggcaact acaagacccg cgccgaggtg
     4681 aagttcgagg gcgacaccct ggtgaaccgc atcgagctga agggcatcga cttcaaggag
     4741 gacggcaaca tcctggggca caagctggag tacaactaca acagccacaa cgtctatatc
     4801 atggccgaca agcagaagaa cggcatcaag gtgaacttca agatccgcca caacatcgag
     4861 gacggcagcg tgcagctcgc cgaccactac cagcagaaca cccccatcgg cgacggcccc
     4921 gtgctgctgc ccgacaacca ctacctgagc acccagtccg ccctgagcaa agaccccaac
     4981 gagaagcgcg atcacatggt cctgctggag ttcgtgaccg ccgccgggat cactctcggc
     5041 atggacgagc tgtacaagta atgaattaat taagaattat caagcttatc gataccgtcg
     5101 acctgcagcc aagcttatcg ataaaataaa agattttatt tagtctccag aaaaaggggg
     5161 gaatgaaaga ccccacctgt aggtttggca agctagctta agtaacgcca ttttgcaagg
     5221 catggaaaat acataactga gaatagagaa gttcagatca aggttaggaa cagagagaca
     5281 gcagaatatg ggccaaacag gatatctgtg gtaagcagtt cctgccccgg ctcagggcca
     5341 agaacagatg gtccccagat gcggtcccgc cctcagcagt ttctagagaa ccatcagatg
     5401 tttccagggt gccccaagga cctgaaatga ccctgtgcct tatttgaact aaccaatcag
     5461 ttcgcttctc gcttctgttc gcgcgcttct gctccccgag ctcaataaaa gagcccacaa
     5521 cccctcactc ggcgcgccag tcctccgata gactgcgtcg cccgggtacc cgtgtatcca
     5581 ataaaccctc ttgcagttgc atccgacttg tggtctcgct gttccttggg agggtctcct
     5641 ctgagtgatt gactacccgt cagcgggggt ctttcatggg taacagtttc ttgaagttgg
     5701 agaacaacat tctgagggta ggagtcgaat attaagtaat cctgactcaa ttagccactg
     5761 ttttgaatcc acatactcca atactcctga aatagttcat tatggacagc gcagaagagc
     5821 tggggagaat taattcgtaa tcatggtcat agctgtttcc tgtgtgaaat tgttatccgc
     5881 tcacaattcc acacaacata cgagccggaa gcataaagtg taaagcctgg ggtgcctaat
     5941 gagtgagcta actcacatta attgcgttgc gctcactgcc cgctttccag tcgggaaacc
     6001 tgtcgtgcca gctgcattaa tgaatcggcc aacgcgcggg gagaggcggt ttgcgtattg
     6061 ggcgctcttc cgcttcctcg ctcactgact cgctgcgctc ggtcgttcgg ctgcggcgag
     6121 cggtatcagc tcactcaaag gcggtaatac ggttatccac agaatcaggg gataacgcag
     6181 gaaagaacat gtgagcaaaa ggccagcaaa aggccaggaa ccgtaaaaag gccgcgttgc
     6241 tggcgttttt ccataggctc cgcccccctg acgagcatca caaaaatcga cgctcaagtc
     6301 agaggtggcg aaacccgaca ggactataaa gataccaggc gtttccccct ggaagctccc
     6361 tcgtgcgctc tcctgttccg accctgccgc ttaccggata cctgtccgcc tttctccctt
     6421 cgggaagcgt ggcgctttct catagctcac gctgtaggta tctcagttcg gtgtaggtcg
     6481 ttcgctccaa gctgggctgt gtgcacgaac cccccgttca gcccgaccgc tgcgccttat
     6541 ccggtaacta tcgtcttgag tccaacccgg taagacacga cttatcgcca ctggcagcag
     6601 ccactggtaa caggattagc agagcgaggt atgtaggcgg tgctacagag ttcttgaagt
     6661 ggtggcctaa ctacggctac actagaagga cagtatttgg tatctgcgct ctgctgaagc
     6721 cagttacctt cggaaaaaga gttggtagct cttgatccgg caaacaaacc accgctggta
     6781 gcggtggttt ttttgtttgc aagcagcaga ttacgcgcag aaaaaaagga tctcaagaag
     6841 atcctttgat cttttctacg gggtctgacg ctcagtggaa cgaaaactca cgttaaggga
     6901 ttttggtcat gagattatca aaaaggatct tcacctagat ccttttaaat taaaaatgaa
     6961 gttttaaatc aatctaaagt atatatgagt aaacttggtc tgacagttac caatgcttaa
     7021 tcagtgaggc acctatctca gcgatctgtc tatttcgttc atccatagtt gcctgactcc
     7081 ccgtcgtgta gataactacg atacgggagg gcttaccatc tggccccagt gctgcaatga
     7141 taccgcgaga cccacgctca ccggctccag atttatcagc aataaaccag ccagccggaa
     7201 gggccgagcg cagaagtggt cctgcaactt tatccgcctc catccagtct attaattgtt
     7261 gccgggaagc tagagtaagt agttcgccag ttaatagttt gcgcaacgtt gttgccattg
     7321 ctacaggcat cgtggtgtca cgctcgtcgt ttggtatggc ttcattcagc tccggttccc
     7381 aacgatcaag gcgagttaca tgatccccca tgttgtgcaa aaaagcggtt agctccttcg
     7441 gtcctccgat cgttgtcaga agtaagttgg ccgcagtgtt atcactcatg gttatggcag
     7501 cactgcataa ttctcttact gtcatgccat ccgtaagatg cttttctgtg actggtgagt
     7561 actcaaccaa gtcattctga gaatagtgta tgcggcgacc gagttgctct tgcccggcgt
     7621 caatacggga taataccgcg ccacatagca gaactttaaa agtgctcatc attggaaaac
     7681 gttcttcggg gcgaaaactc tcaaggatct taccgctgtt gagatccagt tcgatgtaac
     7741 ccactcgtgc acccaactga tcttcagcat cttttacttt caccagcgtt tctgggtgag
     7801 caaaaacagg aaggcaaaat gccgcaaaaa agggaataag ggcgacacgg aaatgttgaa
     7861 tactcatact cttccttttt caatattatt gaagcattta tcagggttat tgtctcatga
     7921 gcggatacat atttgaatgt atttagaaaa ataaacaaat aggggttccg cgcacatttc
     7981 cccgaaaagt gccacctgac gtctaagaaa ccattattat catgacatta acctataaaa
     8041 ataggcgtat cacgaggccc tttcgtctcg cgcgtttcgg tgatgacggt gaaaacctct
     8101 gacacatgca gctcccggag acggtcacag cttgtctgta agcggatgcc gggagcagac
     8161 aagcccgtca gggcgcgtca gcgggtgttg gcgggtgtcg gggctggctt aactatgcgg
     8221 catcagagca gattgtactg agagtgcacc atatgcggtg tgaaataccg cacagatgcg
     8281 taaggagaaa ataccgcatc aggcgccatt cgccattcag gctgcgcaac tgttgggaag
     8341 ggcgatcggt gcgggcctct tcgctattac gccagctggc gaaaggggga tgtgctgcaa
     8401 ggcgattaag ttgggtaacg ccagggtttt cccagtcacg acgttgtaaa acgacggcgc
     8461 aaggaatggt gcatgcaagg agatggcgcc caacagtccc ccggccacgg ggcctgccac
     8521 catacccacg ccgaaacaag cgctcatgag cccgaagtgg cgagcccgat cttccccatc
     8581 ggtgatgtcg gcgatatagg cgccagcaac cgcacctgtg gcgccggtga tgccggccac
     8641 gatgcgtccg gcgtagaggc gattagtcca atttgttaaa gacaggatat cagtggtcca
     8701 ggctctagtt ttgactcaac aatatcacca gctgaagcct atagagtacg agccatagat
     8761 aaaataaaag attttattta gtctccagaa aaagggggga a
//
