LOCUS       sequence-107692-         881 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Localization: Talin Head Domain, Excitation: 488, Emission: 507.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 881)
  TITLE     Davidson Green fluorescent proteins
REFERENCE   2  (bases 1 to 881)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: EGFP-Talin-H-18
FEATURES             Location/Qualifiers
     source          1..881
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      38..47
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(44..46,47..49,50..760)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             join(44..46,47..49,50..760)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(89..110)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(350..369)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     697..718
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     regulatory      809..818
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
ORIGIN
        1 gtttagtgaa ccgtcagatc cgctagcgct accggtcgcc accatggtga gcaagggcga
       61 ggagctgttc accggggtgg tgcccatcct ggtcgagctg gacggcgacg taaacggcca
      121 caagttcagc gtgtccggcg agggcgaggg cgatgccacc tacggcaagc tgaccctgaa
      181 gttcatctgc accaccggca agctgcccgt gccctggccc accctcgtga ccaccctgac
      241 ctacggcgtg cagtgcttca gccgctaccc cgaccacatg aagcagcacg acttcttcaa
      301 gtccgccatg cccgaaggct acgtccagga gcgcaccatc ttcttcaagg acgacggcaa
      361 ctacaagacc cgcgccgagg tgaagttcga gggcgacacc ctggtgaacc gcatcgagct
      421 gaagggcatc gacttcaagg aggacggcaa catcctgggg cacaagctgg agtacaacta
      481 caacagccac aacgtctata tcatggccga caagcagaag aacggcatca aggtgaactt
      541 caagatccgc cacaacatcg aggacggcag cgtgcagctc gccgaccact accagcagaa
      601 cacccccatc ggcgacggcc ccgtgctgct gcccgacaac cactacctga gcacccagtc
      661 cgccctgagc aaagacccca acgagaagcg cgatcacatg gtcctgctgg agttcgtgac
      721 cgccgccggg atcactctcg gcatggacga gctgtacaag tccggactca gatctcgagc
      781 tcaagctagc ttcgaattcg cagaagctgc caccatggtt gcgctttcgc tgaagattag
      841 cattgggaat gtggtgaaga cgatgcaatt tgagccatct a
//
