LOCUS       sequence-107710-        9280 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses MCP-tdiRFP670 in mammalian cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9280)
  AUTHORS   Ochiai H, Sugawara T, Yamamoto T
  TITLE     Simultaneous live imaging of the transcription and nuclear position 
            of specific genes.
  JOURNAL   Nucleic Acids Res. 2015 Jun 19. pii: gkv624.
  PUBMED    26092696
REFERENCE   2  (bases 1 to 9280)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nucleic 
            Acids Res. 2015 Jun 19. pii: gkv624."
COMMENT     Sequence Label: pBSK-Delta-B-CAG-MCP-tdiRFP670-IRES-Neo
FEATURES             Location/Qualifiers
     source          1..9280
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      complement(30..458)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(140..161)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     352..371
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     585..607
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     599..616
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     600..616
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        626..644
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     polyA_signal    754..875
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     819..838
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     CDS             complement(1005..1808)
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MGSAIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQ
                     GRPVLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQD
                     LLSSHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDE
                     EHQGLAPAELFARLKARMPDGDDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQ
                     DIALATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(1116..1135)
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     primer_bind     1726..1745
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     misc_feature    1844..2417
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(2004..2023)
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     primer_bind     2233..2250
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     primer_bind     complement(2488..2507)
                     /label=pMT2-F
                     /note="Synthetic intron, forward primer"
     intron          2490..2719
                     /label=chimeric intron
                     /note="chimera between introns from adenovirus and 
                     immunoglobulin heavy chain genes"
     CDS             complement(5161..5172)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     CDS             complement(5173..5199)
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /note="This is a variant of the standard HA tag, which has 
                     sequence YPYDVPDYA. Some anti-HA antibodies may not 
                     recognize this variant."
                     /translation="YPYDVPDYA"
     CDS             complement(5173..5199)
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     CDS             complement(5203..5223)
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     promoter        complement(5275..5293)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     intron          5391..6399
                     /label=chimeric intron
                     /note="chimera between introns from chicken beta-actin and 
                     rabbit beta-globin"
     primer_bind     complement(5445..5467)
                     /label=pCAGGS-5
                     /note="Chimeric intron in CAG promoter, forward primer"
     promoter        complement(6400..6675)
                     /label=chicken beta-actin promoter
     enhancer        6678..6981
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     primer_bind     complement(7092..7112)
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        complement(7092..7110)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(7131..7147)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(7131..7147)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(7144..7166)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    7155..7171
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(7179..7185,7186..7203,7204..7209))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    7224..7245
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(7362..7379)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(7533..8121)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(7613..7632)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(8292..9083,9084..9152))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     8915..8934
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(9153..9257)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 ctaaattgta agcgttaata ttttgttaaa attcgcgtta aatttttgtt aaatcagctc
       61 attttttaac caataggccg aaatcggcaa aatcccttat aaatcaaaag aatagaccga
      121 gatagggttg agtgttgttc cagtttggaa caagagtcca ctattaaaga acgtggactc
      181 caacgtcaaa gggcgaaaaa ccgtctatca gggcgatggc ccactacgtg aaccatcacc
      241 ctaatcaagt tttttggggt cgaggtgccg taaagcacta aatcggaacc ctaaagggag
      301 cccccgattt agagcttgac ggggaaagcc ggcgaacgtg gcgagaaagg aagggaagaa
      361 agcgaaagga gcgggcgcta gggcgctggc aagtgtagcg gtcacgctgc gcgtaaccac
      421 cacacccgcc gcgcttaatg cgccgctaca gggcgcgtcc cattcgccat tcaggctgcg
      481 caactgttgg gaagggcgat cggtgcgggc ctcttcgcta ttacgccagc tggcgaaagg
      541 gggatgtgct gcaaggcgat taagttgggt aacgccaggg ttttcccagt cacgacgttg
      601 taaaacgacg gccagtgagc gcgcgtaata cgactcacta tagggcgaat tgggtaccgg
      661 gccccccctc gaggtcgacg agacgggtat acagacatga taagatacat tgatgagttt
      721 ggacaaacca caactagaat gcactcgacg cgttaagata cattgatgag tttggacaaa
      781 ccacaactag aatgcagtga aaaaaatgct ttatttgtga aatttgtgat gctattgctt
      841 tatttgtaac cattataagc tgcaataaac aagttaacaa caacaattgc attcatttta
      901 tgtttcaggt tcagggggag gtgtgggagg ttttttaaag caagtaaaac ctctacaaat
      961 gtggtatggc tgattatgat cagttatcta gagaattgat cccctcagaa gaactcgtca
     1021 agaaggcgat agaaggcgat gcgctgcgaa tcgggagcgg cgataccgta aagcacgagg
     1081 aagcggtcag cccattcgcc gccaagctct tcagcaatat cacgggtagc caacgctatg
     1141 tcctgatagc ggtccgccac acccagccgg ccacagtcga tgaatccaga aaagcggcca
     1201 ttttccacca tgatattcgg caagcaggca tcgccatggg tcacgacgag atcatcgccg
     1261 tcgggcatgc gcgccttgag cctggcgaac agttcggctg gcgcgagccc ctgatgctct
     1321 tcgtccagat catcctgatc gacaagaccg gcttccatcc gagtacgtgc tcgctcgatg
     1381 cgatgtttcg cttggtggtc gaatgggcag gtagccggat caagcgtatg cagccgccgc
     1441 attgcatcag ccatgatgga tactttctcg gcaggagcaa ggtgagatga caggagatcc
     1501 tgccccggca cttcgcccaa tagcagccag tcccttcccg cttcagtgac aacgtcgagc
     1561 acagctgcgc aaggaacgcc cgtcgtggcc agccacgata gccgcgctgc ctcgtcctgc
     1621 agttcattca gggcaccgga caggtcggtc ttgacaaaaa gaaccgggcg cccctgcgct
     1681 gacagccgga acacggcggc atcagagcag ccgattgtct gttgtgccca gtcatagccg
     1741 aatagcctct ccacccaagc ggccggagaa cctgcgtgca atccatcttg ttcaatggcc
     1801 gatcccatat tggctgcagg aattatcccg ggttgtggca agcttatcat cgtgtttttc
     1861 aaaggaaaac cacgtccccg tggttcgggg ggcctagacg tttttttaac ctcgactaaa
     1921 cacatgtaaa gcatgtgcac cgaggcccca gatcagatcc catacaatgg ggtaccttct
     1981 gggcatcctt cagccccttg ttgaatacgc ttgaggagag ccatttgact ctttccacaa
     2041 ctatccaact cacaacgtgg cactggggtt gtgccgcctt tgcaggtgta tcttatacac
     2101 gtggcttttg gccgcagagg cacctgtcgc caggtggggg gttccgctgc ctgcaaaggg
     2161 tcgctacaga cgttgtttgt cttcaagaag cttccagagg aactgcttcc ttcacgacat
     2221 tcaacagacc ttgcattcct ttggcgagag gggaaagacc cctaggaatg ctcgtcaaga
     2281 agacagggcc aggtttccgg gccctcacat tgccaaaaga cggcaatatg gtggaaaata
     2341 acatatagac aaacgcacac cggccttatt ccaagcggct tcggccagta acgttagggg
     2401 ggggggaggg aaaagggggg gggggagagg ggcggaattg gccgccctag atgcatgctc
     2461 gacctgcagt tggacctggg agtggacacc tgtggagaga aaggcaaagt ggatgtcatt
     2521 gtcactcaag tgtatggcca gatctcaagc ctgccacacc tcaagcttga caacaaaaag
     2581 attgtctttt ctgaccagat ggacgcggcc accctcaaag gcatcaccgc gggccaggtg
     2641 aatatcaaat cctcctcgtt tttggaaact gacaatctta gcgcagaagt catgcccgct
     2701 tttgagaggg agtactcacc ccaacagctg gccctcgcag acagcgaatt aattccagca
     2761 cactggatca gttatctatg cggccgctca ttaattaacc tcgagtttaa acgcggatcc
     2821 gcgctgatgg tgagcggcag taaaatgaag agacaaaaaa tcggcgaaca tttcggcggc
     2881 cactcgctgt gccattggta ctgctctagg ttcgtaatga tggcaaatta ttaggcccca
     2941 tagtgttcca tcgattatta tgctcaggga catacttgcg ctgaccccca tatttcgcag
     3001 aaactctaaa tgacaagggc tgatagatcg gaggtgagcg aaagacagat ccagcgcagc
     3061 tccagaggcg tcgtgttctg gaacgatcct gctactaata ccgcgggagt ccgataccac
     3121 gcggatggca ttcttgaggt acaaaagcct tgcctgctgg ggtactagag atgcggggaa
     3181 atgctgcccc agaaaagact ccaggtcgga tcgcttggcc tcccctatta ccataccaga
     3241 gccatcgtcg gcgaatctgt agagcattac cctatgataa cccagcattg cctgaagata
     3301 cctgggaaca cgagcggcca tttcttccag cgatttcagc tccttagtgc gggcgataat
     3361 ttgtctggtc aggcgaaggg ggttgtctgc ttgctcagcg gcggccggtt caaactcaat
     3421 tatactggtt ccgtcatgcc ggtgaaggct tatgtcgaaa gtacggcctg tcagtccatc
     3481 tctccaccca aagatgagag cagggcgttt gggatcgctt gattgcgcca gtgcgttcct
     3541 aagtgcgtga gcctcggttt ctccgaaata atcggctaag agttcgccga cacggggcgt
     3601 ttcacggcca aagaaggcac ccgcattttc ggtaattcga gtaatcctga ctgcctgggc
     3661 atcgcaggcc agcaaacacc cacatggctg gatagagcca ggaatgtgta tcggctctcg
     3721 gtcacaactg gtcaggtcca cttttctggc catggccatg ttattatctt ctgatgaagc
     3781 agtcccacta ctgccagaac ctgtcgagcc agtcccgtgg ccgcgctgat ggtgagcggc
     3841 agtaaaatga agagacaaaa aatcggcgaa catttcggcg gccactcgct gtgccattgg
     3901 tactgctcta ggttcgtaat gatggcaaat tattaggccc catagtgttc catcgattat
     3961 tatgctcagg gacatacttg cgctgacccc catatttcgc agaaactcta aatgacaagg
     4021 gctgatagat cggaggtgag cgaaagacag atccagcgca gctccagagg cgtcgtgttc
     4081 tggaacgatc ctgctactaa taccgcggga gtccgatacc acgcggatgg cattcttgag
     4141 gtacaaaagc cttgcctgct ggggtactag agatgcgggg aaatgctgcc ccagaaaaga
     4201 ctccaggtcg gatcgcttgg cctcccctat taccatacca gagccatcgt cggcgaatct
     4261 gtagagcatt accctatgat aacccagcat tgcctgaaga tacctgggaa cacgagcggc
     4321 catttcttcc agcgatttca gctccttagt gcgggcgata atttgtctgg tcaggcgaag
     4381 ggggttgtct gcttgctcag cggcggccgg ttcaaactca attatactgg ttccgtcatg
     4441 ccggtgaagg cttatgtcga aagtacggcc tgtcagtcca tctctccacc caaagatgag
     4501 agcagggcgt ttgggatcgc ttgattgcgc cagtgcgttc ctaagtgcgt gagcctcggt
     4561 ttctccgaaa taatcggcta agagttcgcc gacacggggc gtttcacggc caaagaaggc
     4621 acccgcattt tcggtaattc gagtaatcct gactgcctgg gcatcgcagg ccagcaaaca
     4681 cccacatggc tggatagagc caggaatgtg tatcggctct cggtcacaac tggtcaggtc
     4741 cacttttctg gccatgaatt caccggtctg agtaaagtta gaagccatgg cgtagatgcc
     4801 ggagtttgct gcgattgctg agggaatcgg gtttccatct tttaggagac cttgcattgc
     4861 cttaacaata agctcgcagt cggaattcgt ggcgaaaatt ggaatggtta gttccatatt
     4921 taagtacgaa cgccaggcgc ctttaggcac ctcgactttg atggtgtatt tgcgattctg
     4981 cgcagagctc tgacgaacgc tacaggttac tttgtaagcc tgtgaacgcg agttagagct
     5041 gatccattca gcgatcccgt tagcgaagtt gcttggggcg acagtcacgt cgccagttcc
     5101 gccattgtcg acgagaacga actgagtaaa gttagaagcc attttaacgg ctagcatatg
     5161 gcggccttcg atggcgtagt cgggcacgtc gtaggggtag ccaacttttc ttttcttttt
     5221 tgggcccatg atatcgctag cgacgatccg aggtcgatac caagcttggg tctccctata
     5281 gtgagtcgta ttaatttcga taagccagta agcagtgggt tctctagtta gccagagagc
     5341 tctttgccaa aatgatgaga cagcacaaca accagcacgt tgcccaggag ctgtaggaaa
     5401 aagaagaagg catgaacatg gttagcagag gctctagagc cgccggtcac acgccagaag
     5461 ccgaaccccg ccctgccccg tcccccccga aggcagccgt ccccctgcgg cagccccgag
     5521 gctggagatg gagaagggga cggcggcgcg gcgacgcacg aaggccctcc ccgcccattt
     5581 ccttcctgcc ggcgccgcac cgcttcgccc gcgcccgcta gagggggtgc ggcggcgcct
     5641 cccagatttc ggctccgcca gatttgggac aaaggaagtc cctgcgccct ctcgcacgat
     5701 taccataaaa ggcaatggct gcggctcgcc gcgcctcgac agccgccggc gctccggggc
     5761 cgccgcgccc ctcccccgag ccctccccgg cccgaggcgg ccccgccccg cccggcaccc
     5821 ccacctgccg ccaccccccg cccggcacgg cgagccccgc gccacgcccc gcacggagcc
     5881 ccgcacccga agccgggccg tgctcagcaa ctcggggagg ggggtgcagg ggggggttac
     5941 agcccgaccg ccgcgcccac accccctgct caccccccca cgcacacacc ccgcacgcag
     6001 cctttgttcc cctcgcagcc cccccgcacc gcggggcacc gcccccggcc gcgctcccct
     6061 cgcgcacacg cggagcgcac aaagccccgc gccgcgcccg cagcgctcac agccgccggg
     6121 cagcgcgggc cgcacgcggc gctccccacg cacacacaca cgcacgcacc ccccgagccg
     6181 ctcccccccg cacaaagggc cctcccggag ccctttaagg ctttcacgca gccacagaaa
     6241 agaaacgagc cgtcattaaa ccaagcgcta attacagccc ggaggagaag ggccgtcccg
     6301 cccgctcacc tgtgggagta acgcggtcag tcagagccgg ggcgggcggc gcgaggcggc
     6361 gcggagcggg gcacggggcg aaggcaacgc agcgactccc gcccgccgcg cgcttcgctt
     6421 tttatagggc cgccgccgcc gccgcctcgc cataaaagga aactttcgga gcgcgccgct
     6481 ctgattggct gccgccgcac ctctccgcct cgccccgccc cgcccctcgc cccgccccgc
     6541 cccgcctggc gcgcgccccc cccccccccg cccccatcgc tgcacaaaat aattaaaaaa
     6601 taaataaata caaaattggg ggtggggagg ggggggagat ggggagagtg aagcagaacg
     6661 tggggctcac ctcgacccat ggtaatagcg atgactaata cgtagatgta ctgccaagta
     6721 ggaaagtccc ataaggtcat gtactgggca taatgccagg cgggccattt accgtcattg
     6781 acgtcaatag ggggcgtact tggcatatga tacacttgat gtactgccaa gtgggcagtt
     6841 taccgtaaat actccaccca ttgacgtcaa tggaaagtcc ctattggcgt tactatggga
     6901 acatacgtca ttattgacgt caatgggcgg gggtcgttgg gcggtcagcc aggcgggcca
     6961 tttaccgtaa gttatgtaac gcggaactcc atatatgggc tatgaactaa tgaccccgta
     7021 attgattact attaatacgt ctcactagtt ctagagcggc cgccaccgcg gtggagctcc
     7081 agcttttgtt ccctttagtg agggttaatt gcgcgcttgg cgtaatcatg gtcatagctg
     7141 tttcctgtgt gaaattgtta tccgctcaca attccacaca acatacgagc cggaagcata
     7201 aagtgtaaag cctggggtgc ctaatgagtg agctaactca cattaattgc gttgcgctca
     7261 ctgcccgctt tccagtcggg aaacctgtcg tgccagctgc attaatgaat cggccaacgc
     7321 gcggggagag gcggtttgcg tattgggcgc tcttccgctt cctcgctcac tgactcgctg
     7381 cgctcggtcg ttcggctgcg gcgagcggta tcagctcact caaaggcggt aatacggtta
     7441 tccacagaat caggggataa cgcaggaaag aacatgtgag caaaaggcca gcaaaaggcc
     7501 aggaaccgta aaaaggccgc gttgctggcg tttttccata ggctccgccc ccctgacgag
     7561 catcacaaaa atcgacgctc aagtcagagg tggcgaaacc cgacaggact ataaagatac
     7621 caggcgtttc cccctggaag ctccctcgtg cgctctcctg ttccgaccct gccgcttacc
     7681 ggatacctgt ccgcctttct cccttcggga agcgtggcgc tttctcatag ctcacgctgt
     7741 aggtatctca gttcggtgta ggtcgttcgc tccaagctgg gctgtgtgca cgaacccccc
     7801 gttcagcccg accgctgcgc cttatccggt aactatcgtc ttgagtccaa cccggtaaga
     7861 cacgacttat cgccactggc agcagccact ggtaacagga ttagcagagc gaggtatgta
     7921 ggcggtgcta cagagttctt gaagtggtgg cctaactacg gctacactag aaggacagta
     7981 tttggtatct gcgctctgct gaagccagtt accttcggaa aaagagttgg tagctcttga
     8041 tccggcaaac aaaccaccgc tggtagcggt ggtttttttg tttgcaagca gcagattacg
     8101 cgcagaaaaa aaggatctca agaagatcct ttgatctttt ctacggggtc tgacgctcag
     8161 tggaacgaaa actcacgtta agggattttg gtcatgagat tatcaaaaag gatcttcacc
     8221 tagatccttt taaattaaaa atgaagtttt aaatcaatct aaagtatata tgagtaaact
     8281 tggtctgaca gttaccaatg cttaatcagt gaggcaccta tctcagcgat ctgtctattt
     8341 cgttcatcca tagttgcctg actccccgtc gtgtagataa ctacgatacg ggagggctta
     8401 ccatctggcc ccagtgctgc aatgataccg cggctgccac gctcaccggc tccagattta
     8461 tcagcaataa accagccagc cggaagggcc gagcgcagaa gtggtcctgc aactttatcc
     8521 gcctccatcc agtctattaa ttgttgccgg gaagctagag taagtagttc gccagttaat
     8581 agtttgcgca acgttgttgc cattgctaca ggcatcgtgg tgtcacgctc gtcgtttggt
     8641 atggcttcat tcagctccgg ttcccaacga tcaaggcgag ttacatgatc ccccatgttg
     8701 tgcaaaaaag cggttagctc cttcggtcct ccgatcgttg tcagaagtaa gttggccgca
     8761 gtgttatcac tcatggttat ggcagcactg cataattctc ttactgtcat gccatccgta
     8821 agatgctttt ctgtgactgg tgagtactca accaagtcat tctgagaata gtgtatgcgg
     8881 cgaccgagtt gctcttgccc ggcgtcaata cgggataata ccgcgccaca tagcagaact
     8941 ttaaaagtgc tcatcattgg aaaacgttct tcggggcgaa aactctcaag gatcttaccg
     9001 ctgttgagat ccagttcgat gtaacccact cgtgcaccca actgatcttc agcatctttt
     9061 actttcacca gcgtttctgg gtgagcaaaa acaggaaggc aaaatgccgc aaaaaaggga
     9121 ataagggcga cacggaaatg ttgaatactc atactcttcc tttttcaata ttattgaagc
     9181 atttatcagg gttattgtct catgagcgga tacatatttg aatgtattta gaaaaataaa
     9241 caaatagggg ttccgcgcac atttccccga aaagtgccac
//
