LOCUS       sequence-107713-         962 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  expresses the adaptor protein epsin that lacks its ENTH-domain in 
            bacteria.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 962)
  AUTHORS   Kalthoff C, Alves J, Urbanke C, Knorr R, Ungewickell EJ
  TITLE     Unusual structural organization of the endocytic proteins AP180 and 
            epsin 1.
  JOURNAL   J Biol Chem. 2002 Mar 8;277(10):8209-16. Epub 2001 Dec 26.
  PUBMED    11756460
REFERENCE   2  (bases 1 to 962)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Biol 
            Chem."; date: "2002-03-8"; volume: "277(10)"; pages: "8209-16. Epub 
            2001 Dec 26"
COMMENT     Sequence Label: H6-epsin 1 (144-575)
FEATURES             Location/Qualifiers
     source          1..962
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    16..32
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     protein_bind    48..64
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     RBS             83..94
                     /note="strong bacterial ribosome binding site (Elowitz and 
                     Leibler, 2000)"
     CDS             113..130
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
ORIGIN
        1 aaaaatttat ttgctttgtg agcggataac aattataata gattcaattg tgagcggata
       61 acaatttcac acagaattca ttaaagagga gaaattaact atgagaggat ctcaccatca
      121 ccatcaccat gggatccgca tgcgagctcg gtacccccgc gcccacgcgc tcaagaccaa
      181 ggagaaactg gcacagactg ccacggcttc ctcagcagct gtgggctctg gaccacctcc
      241 cgaggcggag caggcatggc cacagagcag cggggaggag gagttgcagc tccagctggc
      301 cctggctatg agcaaggagg aggctgacca gcccccgtcc tgcggccccg aggatgacgt
      361 ccagctccag ctggccctta gtttgagccg agaggagcac gataaggaag agcggatccg
      421 tcgtggggat gacctgaggc tgcagatggc aatagaggag agcaagaggg agactggggg
      481 caaggaggag tcatctctta tggatcttgc tgacgtcttc acaaccccag cccctccaca
      541 ggcctcagac ccttgggggg gcccggcatc tgtgcctact gctgtccctg tggctgctgc
      601 tgcctcagac ccttgggggg cacctgctgt ccctccagct gctgatcctt ggggtggtgc
      661 agcccccacg ccagcctccg gggatccttg gaggcctgca gcccctacag ggccctctgt
      721 tgacccttgg ggtgggaccc cagctcctgc agctggagag gggcccacat ctgacccgtg
      781 gggaagcgct gatggtgggg cccctgtcag tggaccccca tcttctgacc cctgggcacc
      841 tgccccagct ttctcagacc cttggggagg ttcacctgca aagcccagca gcaatggcac
      901 tgcagttggg ggctttgaca ctgagcccga tgagttctca gacttcgacc gactccgcac
      961 tg
//
