LOCUS       sequence-107715-         956 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  6xHis-tagged full-length bovine brain auxilin (auxilin 1) in pQE30 
            for protein expression in bacteria.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 956)
  AUTHORS   Schroder S, Morris SA, Knorr R, Plessmann U, Weber K, Nguyen GV, 
            Ungewickell E
  TITLE     Primary structure of the neuronal clathrin-associated protein 
            auxilin and its expression in bacteria.
  JOURNAL   Eur J Biochem. 1995 Mar 1;228(2):297-304.
  PUBMED    7705342
REFERENCE   2  (bases 1 to 956)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Eur J 
            Biochem."; date: "1995-03-1"; volume: "228(2)"; pages: "297-304"
COMMENT     Sequence Label: H6-auxilin
FEATURES             Location/Qualifiers
     source          1..956
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        join(3..17,18..23,24..40,41..47)
                     /label=T5 promoter
                     /note="bacteriophage T5 promoter for E. coli RNA 
                     polymerase, with embedded lac operator"
     protein_bind    23..39
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     protein_bind    55..71
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     RBS             90..101
                     /note="strong bacterial ribosome binding site (Elowitz and 
                     Leibler, 2000)"
     CDS             120..137
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
ORIGIN
        1 aatcataaaa aatttatttg ctttgtgagc ggataacaat tataatagat tcaattgtga
       61 gcggataaca atttcacaca gaattcatta aagaggagaa attaactatg agaggatcgc
      121 atcaccatca ccatcacgga tccgcatgcg agctcggtac ccccgccagc accatggaca
      181 gctcaggtgc ctcatctcca gacatggagc ccagctatgg cggaggtctt tttgacatgg
      241 tgaaaggagg ggcagggagg ctctttagca acctaaagga caacttgaaa gacactctca
      301 aagacacgtc ttccagagtt atacaatctg ttaccagcta cacgaaagga gatttagact
      361 tcacttatgt tacctccaga attattgtga tgtcctttcc tctggacagt gttgacatag
      421 gattcaggaa tcaggttgat gatattcgaa gctttttgga ttccagacat cttgaccact
      481 acacggtata caatctgtcg cccaagtctt atcgaactgc caagtttcac agcagggtct
      541 cagaatgcag ttggcccatc aggcaggccc ccagcctgca caacctcttt gctgtgtgtc
      601 ggaatatgta taactggcta ctacagaatc ccaaaaacgt ctgtgttgtc cactgcttgg
      661 atggacgggc agcatcatcg attctggttg gtgctatgtt cattttctgt aatctctact
      721 ctactcctgg cccagccgtt cgattgctct acgcaaagcg accaggaatt ggactttcac
      781 catcccacag gaggtacctg ggctacatgt gtgacctcct agcagacaaa ccctaccgcc
      841 ctcacttcaa gcctctcaca attaagtcga tcattgtcag tccagttcct tttttcaaca
      901 aacagagaaa tggatgtcgc ccttactgcg atgtactgat tggagaaacc aaaata
//
