LOCUS       sequence-10774-e         837 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 837)
  AUTHORS   Johannessen CM, Boehm JS, Kim SY, Thomas SR, Wardwell L, Johnson LA,
            Emery CM, Stransky N, Cogdill AP, Barretina J, Caponigro G, 
            Hieronymus H, Murray RR, Salehi-Ashtiani K, Hill DE, Vidal M, Zhao 
            JJ, Yang X, Alkan O, Kim S, Harris JL, Wilson CJ, Myer VE, Finan PM,
            Root DE, Roberts TM, Golub T, Flaherty KT, Dummer R, Weber BL, 
            Sellers WR, Schlegel R, Wargo JA, Hahn WC, Garraway LA
  TITLE     COT drives resistance to RAF inhibition through MAP kinase pathway 
            reactivation.
  JOURNAL   Nature. 2010 Dec 16;468(7326):968-72. doi: 10.1038/nature09627
  PUBMED    21107320
REFERENCE   2  (bases 1 to 837)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10"; journalName: 
            "Nature"; date: "2010-12-16- 16"; volume: "468"; issue: "7326"; 
            pages: "968-72"
COMMENT     Sequence Label: pDONR223-PRKCE
FEATURES             Location/Qualifiers
     source          1..837
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    79..103
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             753..764
                     /codon_start=1
                     /gene="cspA"
                     /product="translation enhancing element for E. coli (Qing 
                     et al., 2004)"
                     /label=TEE
                     /translation="NHKV"
ORIGIN
        1 ccccaaataa gtgattttat tttgactgat agtgacctgt tcgttgcaca aattgatgag
       61 caatgctttt ttataatgcc aactttgtac aaaaaagttg gcaccatggt agtgttcaat
      121 ggccttctta agatcaaaat ctgcgaggcc gtgagcttga agcccacagc ctggtcgctg
      181 cgccatgcgg tgggaccccg gccgcagact ttccttctcg acccctacat tgccctcaat
      241 gtggacgact cgcgcatcgg ccaaacggcc accaagcaga agaccaacag cccggcctgg
      301 cacgacgagt tcgtcaccga tgtgtgcaac ggacgcaaga tcgagctggc tgtctttcac
      361 gatgccccca taggctacga cgacttcgtg gccaactgca ccatccagtt tgaggagctg
      421 ctgcagaacg ggagccgcca cttcgaggac tggattgatc tggagccaga aggaagagtg
      481 tatgtgatca tcgatctctc agggtcgtcg ggtgaagccc ctaaagacaa tgaagagcgt
      541 gtgttcaggg aacgcatgcg gccgaggaag cggcaggggg ccgtcaggcg cagggtccat
      601 caggtcaacg gccacaagtt catggccacc tatcttcggc agcccaccta ctgctcccat
      661 tgcagagact tcatctgggg tgtcatagga aagcagggat accagtgtca agtctgcacc
      721 tgcgtggtcc acaagcggtg ccacgagctc ataatcacaa agtgtgctgg ggttaagaag
      781 caggaaaccc ccgaacaggt gggctcccag cggttcaacc gtcacatggc cccaaag
//
