LOCUS       sequence-107752-         939 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Localization: Endoplasmic Reticulum, Excitation: 558, Emission: 583.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 939)
  AUTHORS   Day RN, Davidson MW
  TITLE     The fluorescent protein palette: tools for cellular imaging.
  JOURNAL   Chem Soc Rev. 2009 Oct;38(10):2887-921. doi: 10.1039/b901966a. Epub 
            2009 Aug 4.
  PUBMED    19771335
REFERENCE   2  (bases 1 to 939)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1039/b901966a"; 
            journalName: "Chem Soc Rev"; date: "2009-10"; volume: "38"; issue: 
            "10"; pages: "2887-921"
COMMENT     Sequence Label: DsRed2-ER-5
FEATURES             Location/Qualifiers
     source          1..939
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             104..778
                     /codon_start=1
                     /product="improved tetrameric variant of DsRed fluorescent 
                     protein"
                     /label=DsRed2
                     /note="mammalian codon-optimized"
                     /translation="MASSENVITEFMRFKVRMEGTVNGHEFEIEGEGEGRPYEGHNTVK
                     LKVTKGGPLPFAWDILSPQFQYGSKVYVKHPADIPDYKKLSFPEGFKWERVMNFEDGGV
                     ATVTQDSSLQDGCFIYKVKFIGVNFPSDGPVMQKKTMGWEASTERLYPRDGVLKGETHK
                     ALKLKDGGHYLVEFKSIYMAKKPVQLPGYYYVDAKLDITSHNEDYTIVEQYERTEGRHH
                     LFL"
     primer_bind     complement(284..304)
                     /label=DsRed1-N
                     /note="DsRed1, reverse primer"
     primer_bind     696..719
                     /label=DsRed1-C
                     /note="DsRed1, forward primer"
     misc_feature    806..862
                     /label=MCS
                     /note="multiple cloning site"
ORIGIN
        1 agcagagctg gtttagtgaa ccgtcagatc cgctagcatg ctgctatccg tgccgttgct
       61 gctcggcctc ctcggcctgg ccgtcgccga ccggtcgcac accatggcct cctccgagaa
      121 cgtcatcacc gagttcatgc gcttcaaggt gcgcatggag ggcaccgtga acggccacga
      181 gttcgagatc gagggcgagg gcgagggccg cccctacgag ggccacaaca ccgtgaagct
      241 gaaggtgacc aagggcggcc ccctgccctt cgcctgggac atcctgtccc cccagttcca
      301 gtacggctcc aaggtgtacg tgaagcaccc cgccgacatc cccgactaca agaagctgtc
      361 cttccccgag ggcttcaagt gggagcgcgt gatgaacttc gaggacggcg gcgtggcgac
      421 cgtgacccag gactcctccc tgcaggacgg ctgcttcatc tacaaggtga agttcatcgg
      481 cgtgaacttc ccctccgacg gccccgtgat gcagaagaag accatgggct gggaggcctc
      541 caccgagcgc ctgtaccccc gcgacggcgt gctgaagggc gagacccaca aggccctgaa
      601 gctgaaggac ggcggccact acctggtgga gttcaagtcc atctacatgg ccaagaagcc
      661 cgtgcagctg cccggctact actacgtgga cgccaagctg gacatcacct cccacaacga
      721 ggactacacc atcgtggagc agtacgagcg caccgagggc cgccaccacc tgttcctgag
      781 atcgtacaag aaggacgagc tgtaaagatc tcgagctcaa gcttcgaatt ctgcagtcga
      841 cggtaccgcg ggcccgggat ccaccggatc tagataactg atcataatca gccataccac
      901 atttgtagag gttttacttg ctttaaaaaa cctcccaca
//
