LOCUS       sequence-107758-         898 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  pRS403 backbone, contains an RFP flanked by BsaI sites with CAGT.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 898)
  AUTHORS   Agmon N, Mitchell LA, Cai Y, Ikushima S, Chuang J, Zheng A, Choi WJ,
            Martin JA, Caravelli K, Stracquadanio G, Boeke JD
  TITLE     Yeast Golden Gate (yGG) for the Efficient Assembly of S. cerevisiae 
            Transcription Units.
  JOURNAL   ACS Synth Biol. 2015 Jul 17;4(7):853-9. doi: 10.1021/sb500372z. Epub
            2015 Mar 23.
  PUBMED    25756291
REFERENCE   2  (bases 1 to 898)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1021/sb500372z";
            journalName: "ACS Synth Biol"; date: "2015-07-17- 17"; volume: "4"; 
            issue: "7"; pages: "853-9"
COMMENT     Sequence Label: pAV103
FEATURES             Location/Qualifiers
     source          1..898
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     39..55
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     primer_bind     40..56
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     CDS             complement(140..817)
                     /codon_start=1
                     /product="monomeric derivative of DsRed (Campbell et al., 
                     2002)"
                     /label=mRFP1
                     /translation="MASSEDVIKEFMRFKVRMEGSVNGHEFEIEGEGEGRPYEGTQTAK
                     LKVTKGGPLPFAWDILSPQFQYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNFEDGGV
                     VTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASTERMYPEDGALKGEIKM
                     RLKLKDGGHYDAEVKTTYMAKKPVQLPGAYKTDIKLDITSHNEDYTIVEQYERAEGRHS
                     TGA"
     RBS             824..835
                     /note="strong bacterial ribosome binding site (Elowitz and 
                     Leibler, 2000)"
     protein_bind    853..869
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
ORIGIN
        1 cgactcacta tagggcgaat tgggtaccgg gccccccctc gaggtcgacg gtatcgataa
       61 gcttgatatc gaattcctgc agcccccagt agagaccgcc tggctctagt agcgatctac
      121 actagcacta tcagcgttat taagcaccgg tggagtgacg accttcagca cgttcgtact
      181 gttcaacgat ggtgtagtct tcgttgtggg aggtgatgtc cagtttgatg tcggttttgt
      241 aagcacccgg cagctgaacc ggttttttag ccatgtaggt ggttttaact tcagcgtcgt
      301 agtgaccacc gtctttcagt ttcagacgca ttttgatttc acctttcaga gcaccgtctt
      361 ccgggtacat acgttcggtg gaagcttccc aacccatggt ttttttctgc ataaccggac
      421 cgtcggacgg gaagttggta ccacgcagtt taactttgta gatgaactca ccgtcttgca
      481 gggaggagtc ctgggtaacg gtaacaacac caccgtcttc gaagttcata acacgttccc
      541 atttgaaacc ttccgggaag gacagtttca ggtagtccgg gatgtcagcc gggtgtttaa
      601 cgtaagcttt ggaaccgtac tggaactgcg gggacaggat gtcccaagcg aacggcagcg
      661 gaccaccttt ggtaactttc agtttagcgg tctgggtacc ttcgtacgga cgaccttcac
      721 cttcaccttc gatttcgaac tcgtgaccgt taacggaacc ttccatacga actttgaaac
      781 gcatgaactc tttgataacg tcttcggagg aagccatcta gtatttctcc tctttctcta
      841 gtatgtgtga aattgttatc cgctcacaat tccacacaac atacgagccg gaagcata
//
