LOCUS       sequence-107759-         909 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  pRS404 backbone, contains an RFP flanked by BsaI sites with CAGT.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 909)
  AUTHORS   Agmon N, Mitchell LA, Cai Y, Ikushima S, Chuang J, Zheng A, Choi WJ,
            Martin JA, Caravelli K, Stracquadanio G, Boeke JD
  TITLE     Yeast Golden Gate (yGG) for the Efficient Assembly of S. cerevisiae 
            Transcription Units.
  JOURNAL   ACS Synth Biol. 2015 Jul 17;4(7):853-9. doi: 10.1021/sb500372z. Epub
            2015 Mar 23.
  PUBMED    25756291
REFERENCE   2  (bases 1 to 909)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1021/sb500372z";
            journalName: "ACS Synth Biol"; date: "2015-07-17- 17"; volume: "4"; 
            issue: "7"; pages: "853-9"
COMMENT     Sequence Label: pAV104
FEATURES             Location/Qualifiers
     source          1..909
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     38..54
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     primer_bind     39..55
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     CDS             complement(139..816)
                     /codon_start=1
                     /product="monomeric derivative of DsRed (Campbell et al., 
                     2002)"
                     /label=mRFP1
                     /translation="MASSEDVIKEFMRFKVRMEGSVNGHEFEIEGEGEGRPYEGTQTAK
                     LKVTKGGPLPFAWDILSPQFQYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNFEDGGV
                     VTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASTERMYPEDGALKGEIKM
                     RLKLKDGGHYDAEVKTTYMAKKPVQLPGAYKTDIKLDITSHNEDYTIVEQYERAEGRHS
                     TGA"
     RBS             823..834
                     /note="strong bacterial ribosome binding site (Elowitz and 
                     Leibler, 2000)"
     protein_bind    852..868
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(876..882,883..900,901..906))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
ORIGIN
        1 gactcactat agggcgaatt gggtaccggg ccccccctcg aggtcgacgg tatcgataag
       61 cttgatatcg aattcctgca gcccccagta gagaccgcct ggctctagta gcgatctaca
      121 ctagcactat cagcgttatt aagcaccggt ggagtgacga ccttcagcac gttcgtactg
      181 ttcaacgatg gtgtagtctt cgttgtggga ggtgatgtcc agtttgatgt cggttttgta
      241 agcacccggc agctgaaccg gttttttagc catgtaggtg gttttaactt cagcgtcgta
      301 gtgaccaccg tctttcagtt tcagacgcat tttgatttca cctttcagag caccgtcttc
      361 cgggtacata cgttcggtgg aagcttccca acccatggtt tttttctgca taaccggacc
      421 gtcggacggg aagttggtac cacgcagttt aactttgtag atgaactcac cgtcttgcag
      481 ggaggagtcc tgggtaacgg taacaacacc accgtcttcg aagttcataa cacgttccca
      541 tttgaaacct tccgggaagg acagtttcag gtagtccggg atgtcagccg ggtgtttaac
      601 gtaagctttg gaaccgtact ggaactgcgg ggacaggatg tcccaagcga acggcagcgg
      661 accacctttg gtaactttca gtttagcggt ctgggtacct tcgtacggac gaccttcacc
      721 ttcaccttcg atttcgaact cgtgaccgtt aacggaacct tccatacgaa ctttgaaacg
      781 catgaactct ttgataacgt cttcggagga agccatctag tatttctcct ctttctctag
      841 tatgtgtgaa attgttatcc gctcacaatt ccacacaaca tacgagccgg aagcataaag
      901 tgtaaagcc
//
