LOCUS       sequence-107763-         851 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  pCACS backbone (Amp), SpHIS5, contains an RFP flanked by BsaI sites 
            with CAGT.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 851)
  AUTHORS   Agmon N, Mitchell LA, Cai Y, Ikushima S, Chuang J, Zheng A, Choi WJ,
            Martin JA, Caravelli K, Stracquadanio G, Boeke JD
  TITLE     Yeast Golden Gate (yGG) for the Efficient Assembly of S. cerevisiae 
            Transcription Units.
  JOURNAL   ACS Synth Biol. 2015 Jul 17;4(7):853-9. doi: 10.1021/sb500372z. Epub
            2015 Mar 23.
  PUBMED    25756291
REFERENCE   2  (bases 1 to 851)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1021/sb500372z";
            journalName: "ACS Synth Biol"; date: "2015-07-17- 17"; volume: "4"; 
            issue: "7"; pages: "853-9"
COMMENT     Sequence Label: pAV10.F3.loxP
FEATURES             Location/Qualifiers
     source          1..851
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    complement(169..202)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     terminator      complement(241..438)
                     /label=TEF terminator
                     /note="Ashbya gossypii TEF terminator"
ORIGIN
        1 gctgccgggt gcttacaaaa ccgacatcaa actggacatc acctcccaca acgaagacta
       61 caccatcgtt gaacagtacg aacgtgctga aggtcgtcac tccaccggtg cttaataacg
      121 ctgatagtgc tagtgtagat cgctactaga gccaggcggt ctctactgat aacttcgtat
      181 agcatacatt atacgaagtt attgctagcc tggatggcgg cgttagtatc gaatcgacag
      241 cagtatagcg accagcattc acatacgatt gacgcatgat attactttct gcgcacttaa
      301 cttcgcatct gggcagatga tgtcgaggcg aaaaaaaata taaatcacgc taacatttga
      361 ttaaaataga acaactacaa tataaaaaaa ctatacaaat gacaagttct tgaaaacaag
      421 aatcttttta ttgtcagtac tctttacaac actcccttcg tgcttgggac ttcagaactt
      481 ccagtaagac tagtagccgc gcgcatggca acagccagag atttaaaagc gctttcagca
      541 cgatgatggt cattactacc atataagcag gtaacatgca aagtaattcc agctgctacc
      601 gaaaaggaat atagtaagtg agggatcatt tcacaggaca attccccaac cttttcacgc
      661 tttaatccca aatcgataac agcatagggc cgtcccgaca agtcaactac gcttctagaa
      721 agggcttcgt caagtggaca ataagcatgt ccaaatcttt taacgccggc aaagttaccc
      781 atagcctgct tgaatgcaat accaagtgca atagcagtat cttctgcagt gtgatgatca
      841 tcgatgatta a
//
