LOCUS       sequence-107779-         986 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses tdTomato under the UbC promoter. This promoter expresses 
            transgenes in neurons at higher levels than plasmids with the CMV. 
            Optimal for tracing axons in tissue clearing procedures.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 986)
  AUTHORS   Soderblom C, Lee DH, Dawood A, Carballosa M, Jimena Santamaria A, 
            Benavides FD, Jergova S, Grumbles RM, Thomas CK, Park KK, Guest JD, 
            Lemmon VP, Lee JK, Tsoulfas P
  TITLE     3D Imaging of Axons in Transparent Spinal Cords from Rodents and 
            Nonhuman Primates
  JOURNAL   Eneuro. 2015 Mar-Apr;2(2). doi: 10.1523/ENEURO.0001-15.2015.
  PUBMED    26023683
REFERENCE   2  (bases 1 to 986)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Eneuro. 
            2015 Mar-Apr"; volume: "2(2). doi"; pages: " 
            10.1523/ENEURO.0001-15.2015"
COMMENT     Sequence Label: pAAV-UbC-tdTomato
FEATURES             Location/Qualifiers
     source          1..986
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     100..119
                     /label=pCAG-F
                     /note="Rabbit beta-globin intron, for pCAG plasmids, 
                     forward primer"
     regulatory      142..151
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
ORIGIN
        1 gatatacact gtttgagatg aggataaaat actctgagtc caaaccgggc ccctctgcta
       61 accatgttca tgccttcttc tttttcctac agctcctggg caacgtgctg gttattgtgc
      121 tgtctcatca agcttggatc cgccaccatg gtgtccaagg gcgaggaagt gatcaaagaa
      181 ttcatgcggt tcaaagtgcg gatggaaggc agcatgaacg gccacgagtt cgagatcgag
      241 ggcgaggggg agggcagacc ctacgagggc acacagaccg ccaagctgaa agtgacaaag
      301 ggcggacccc tgcccttcgc ctgggacatc ctgagccccc agtttatgta cggcagcaag
      361 gcctacgtga agcaccccgc cgacatcccc gactacaaga agctgagctt ccccgagggc
      421 ttcaagtggg agagagtgat gaacttcgag gacggcggcc tggtgacagt gacccaggac
      481 agcagcctgc aggacggcac cctgatctac aaagtgaaga tgcggggcac caacttcccc
      541 cccgacggcc ccgtgatgca gaaaaagacc atgggctggg aggccagcac cgagcggctg
      601 taccctaggg atggcgtgct gaagggcgag atccaccagg ccctgaagct gaaggatggc
      661 ggccactacc tggtggaatt caagaccatc tacatggcca agaaacccgt gcagctgcct
      721 ggctactact acgtggacac caagctggac atcaccagcc acaacgagga ctacaccatc
      781 gtggaacagt acgagcggag cgagggccgg caccacctgt ttctgggcca cggcacaggc
      841 agcaccggca gcggaagctc tggcaccgcc agcagcgagg acaacaacat ggccgtgatc
      901 aaagaattca tgcgctttaa agtgcgcatg gaaggctcta tgaatgggca cgagtttgaa
      961 attgaggggg aaggcgaggg acggcc
//
