LOCUS       sequence-107783-        1028 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses GFP under the UbC promoter. This promoter expresses 
            transgenes in neurons at higher levels than plasmids with the CMV. 
            Optimal for tracing axons in tissue clearing procedures. .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1028)
  AUTHORS   Soderblom C, Lee DH, Dawood A, Carballosa M, Jimena Santamaria A, 
            Benavides FD, Jergova S, Grumbles RM, Thomas CK, Park KK, Guest JD, 
            Lemmon VP, Lee JK, Tsoulfas P
  TITLE     3D Imaging of Axons in Transparent Spinal Cords from Rodents and 
            Nonhuman Primates
  JOURNAL   Eneuro. 2015 Mar-Apr;2(2). doi: 10.1523/ENEURO.0001-15.2015.
  PUBMED    26023683
REFERENCE   2  (bases 1 to 1028)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Eneuro. 
            2015 Mar-Apr"; volume: "2(2). doi"; pages: " 
            10.1523/ENEURO.0001-15.2015"
COMMENT     Sequence Label: pAAV-UbC-eGFP
FEATURES             Location/Qualifiers
     source          1..1028
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     678..697
                     /label=pCAG-F
                     /note="Rabbit beta-globin intron, for pCAG plasmids, 
                     forward primer"
     primer_bind     complement(770..791)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
ORIGIN
        1 gtgtgttttg tgaagttttt taggcacctt ttgaaatgta atcatttggg tcaatatgta
       61 attttcagtg ttagactagt aaattgtccg ctaaattctg gccgtttttg gcttttttgt
      121 tagacgaagc taaggcgcgc ctgagaactt cagggtgagt ttggggaccc ttgattgttc
      181 tttctttttc gctattgtaa aattcatgtt atatggaggg ggcaaagttt tcagggtgtt
      241 gtttagaatg ggaagatgtc ccttgtatca ccatgcatgg accctcatga taattttgtt
      301 tctttcactt tctactctgt tgacaaccat tgtctcctct tattttcttt tcattttctg
      361 taactttttc gttaaacttt agcttgcatt tgtaacgaat ctagacaatc agggtatatt
      421 atattgtact tcagcacagt tttagagaac aattgttata attaaatgat aaggtagaat
      481 atttctgcat ataaattctg gctggcgtgg aaatattctt attggtagaa acaactacat
      541 cctggtcatc atcctgcctt tctctttatg gttacaatga tatacactgt ttgagatgag
      601 gataaaatac tctgagtcca aaccgggccc ctctgctaac catgttcatg ccttcttctt
      661 tttcctacag ctcctgggca acgtgctggt tattgtgctg tctcatcaag cttaaggatc
      721 caccatggtg agcaagggcg aggagctgtt caccggggtg gtgcccatcc tggtcgagct
      781 ggacggcgac gtaaacggcc acaagttcag cgtgtccggc gagggcgagg gcgatgccac
      841 ctacggcaag ctgaccctga agttcatctg caccaccggc aagctgcccg tgccctggcc
      901 caccctcgtg accaccctga cctacggcgt gcagtgcttc agccgctacc ccgaccacat
      961 gaagcagcac gacttcttca agtccgccat gcccgaaggc tacgtccagg agcgcaccat
     1021 cttcttca
//
