LOCUS       sequence-107788-         864 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  pCACS backbone (Amp), SpHIS5, contains an RFP flanked by BsaI sites 
            with CAGT.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 864)
  AUTHORS   Agmon N, Mitchell LA, Cai Y, Ikushima S, Chuang J, Zheng A, Choi WJ,
            Martin JA, Caravelli K, Stracquadanio G, Boeke JD
  TITLE     Yeast Golden Gate (yGG) for the Efficient Assembly of S. cerevisiae 
            Transcription Units.
  JOURNAL   ACS Synth Biol. 2015 Jul 17;4(7):853-9. doi: 10.1021/sb500372z. Epub
            2015 Mar 23.
  PUBMED    25756291
REFERENCE   2  (bases 1 to 864)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1021/sb500372z";
            journalName: "ACS Synth Biol"; date: "2015-07-17- 17"; volume: "4"; 
            issue: "7"; pages: "853-9"
COMMENT     Sequence Label: pAV10.HO3.loxP
FEATURES             Location/Qualifiers
     source          1..864
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    complement(166..199)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     terminator      complement(238..435)
                     /label=TEF terminator
                     /note="Ashbya gossypii TEF terminator"
ORIGIN
        1 gccgggtgct tacaaaaccg acatcaaact ggacatcacc tcccacaacg aagactacac
       61 catcgttgaa cagtacgaac gtgctgaagg tcgtcactcc accggtgctt aataacgctg
      121 atagtgctag tgtagatcgc tactagagcc aggcggtctc tactgataac ttcgtatagc
      181 atacattata cgaagttatt gctagcctgg atggcggcgt tagtatcgaa tcgacagcag
      241 tatagcgacc agcattcaca tacgattgac gcatgatatt actttctgcg cacttaactt
      301 cgcatctggg cagatgatgt cgaggcgaaa aaaaatataa atcacgctaa catttgatta
      361 aaatagaaca actacaatat aaaaaaacta tacaaatgac aagttcttga aaacaagaat
      421 ctttttattg tcagtactct ttacaacact cccttcgtgc ttgggacttc agaacttcca
      481 gtaagactag tagccgcgcg catggcaaca gccagagatt taaaagcgct ttcagcacga
      541 tgatggtcat tactaccata taagcaggta acatgcaaag taattccagc tgctaccgaa
      601 aaggaatata gtaagtgagg gatcatttca caggacaatt ccccaacctt ttcacgcttt
      661 aatcccaaat cgataacagc atagggccgt cccgacaagt caactacgct tctagaaagg
      721 gcttcgtcaa gtggacaata agcatgtcca aatcttttaa cgccggcaaa gttacccata
      781 gcctgcttga atgcaatacc aagtgcaata gcagtatctt ctgcagtgtg atgatcatcg
      841 atgattaaat cacctcttga gtaa
//
