LOCUS       sequence-107794-         853 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  pCACS backbone (Amp), Hyg, contains an RFP flanked by BsaI sites 
            with CAGT.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 853)
  AUTHORS   Agmon N, Mitchell LA, Cai Y, Ikushima S, Chuang J, Zheng A, Choi WJ,
            Martin JA, Caravelli K, Stracquadanio G, Boeke JD
  TITLE     Yeast Golden Gate (yGG) for the Efficient Assembly of S. cerevisiae 
            Transcription Units.
  JOURNAL   ACS Synth Biol. 2015 Jul 17;4(7):853-9. doi: 10.1021/sb500372z. Epub
            2015 Mar 23.
  PUBMED    25756291
REFERENCE   2  (bases 1 to 853)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1021/sb500372z";
            journalName: "ACS Synth Biol"; date: "2015-07-17- 17"; volume: "4"; 
            issue: "7"; pages: "853-9"
COMMENT     Sequence Label: pAV10.KH
FEATURES             Location/Qualifiers
     source          1..853
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     terminator      complement(219..416)
                     /label=TEF terminator
                     /note="Ashbya gossypii TEF terminator"
ORIGIN
        1 gctgccgggt gcttacaaaa ccgacatcaa actggacatc acctcccaca acgaagacta
       61 caccatcgtt gaacagtacg aacgtgctga aggtcgtcac tccaccggtg cttaataacg
      121 ctgatagtgc tagtgtagat cgctactaga gccaggcggt ctctactgtg ctagctcgtt
      181 ttcgacactg gatggcggcg ttagtatcga atcgacagca gtatagcgac cagcattcac
      241 atacgattga cgcatgatat tactttctgc gcacttaact tcgcatctgg gcagatgatg
      301 tcgaggcgaa aaaaaatata aatcacgcta acatttgatt aaaatagaac aactacaata
      361 taaaaaaact atacaaatga caagttcttg aaaacaagaa tctttttatt gtcagtactg
      421 attattcctt tgccctcgga cgagtgctgg ggcgtcggtt tccactatcg gcgagtactt
      481 ctacacagcc atcggtccag acggccgcgc ttctgcgggc gatttgtgta cgcccgacag
      541 tcccggctcc ggatcggacg attgcgtcgc atcgaccctg cgcccaagct gcatcatcga
      601 aattgccgtc aaccaagctc tgatagagtt ggtcaagacc aatgcggagc atatacgccc
      661 ggagccgcgg cgatcctgca agctccggat gcctccgctc gaagtagcgc gtctgctgct
      721 ccatacaagc caaccacggc ctccagaaga agatgttggc gacctcgtat tgggaatccc
      781 cgaacatcgc ctcgctccag tcaatgaccg ctgttatgcg gccattgtcc gtcaggacat
      841 tgttggagcc gaa
//
