LOCUS       sequence-107796-         797 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  pCACS backbone (Amp), cloNAT, contains an RFP flanked by BsaI sites 
            with CAGT.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 797)
  AUTHORS   Agmon N, Mitchell LA, Cai Y, Ikushima S, Chuang J, Zheng A, Choi WJ,
            Martin JA, Caravelli K, Stracquadanio G, Boeke JD
  TITLE     Yeast Golden Gate (yGG) for the Efficient Assembly of S. cerevisiae 
            Transcription Units.
  JOURNAL   ACS Synth Biol. 2015 Jul 17;4(7):853-9. doi: 10.1021/sb500372z. Epub
            2015 Mar 23.
  PUBMED    25756291
REFERENCE   2  (bases 1 to 797)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1021/sb500372z";
            journalName: "ACS Synth Biol"; date: "2015-07-17- 17"; volume: "4"; 
            issue: "7"; pages: "853-9"
COMMENT     Sequence Label: pAV10.KN
FEATURES             Location/Qualifiers
     source          1..797
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     terminator      complement(203..400)
                     /label=TEF terminator
                     /note="Ashbya gossypii TEF terminator"
ORIGIN
        1 gcttacaaaa ccgacatcaa actggacatc acctcccaca acgaagacta caccatcgtt
       61 gaacagtacg aacgtgctga aggtcgtcac tccaccggtg cttaataacg ctgatagtgc
      121 tagtgtagat cgctactaga gccaggcggt ctctactgtg ctagctcgac actggatggc
      181 ggcgttagta tcgaatcgac agcagtatag cgaccagcat tcacatacga ttgacgcatg
      241 atattacttt ctgcgcactt aacttcgcat ctgggcagat gatgtcgagg cgaaaaaaaa
      301 tataaatcac gctaacattt gattaaaata gaacaactac aatataaaaa aactatacaa
      361 atgacaagtt cttgaaaaca agaatctttt tattgtcagt actgattagg ggcagggcat
      421 gctcatgtag agcgcctgct cgccgtccga ggcggtgccg tcgtacaggg cggtgtccag
      481 gccgcagagg gtgaacccca tccgccggta cgcgtggatc gccggtgcgt tgacgttggt
      541 gacctccagc cagaggtgcc cggcgccccg ctcgcgggcg aactccgtcg cgagccccat
      601 caacgcgcgc ccgaccccgt gcccccggtg ctccggggcg acctcgatgt cctcgacggt
      661 cagccggcgg ttccagccgg agtacgaaac aaccacgaag cccgccaggt cgccgtcgtc
      721 cccgtacgcg acgaacgtcc gggagtccgg gtcgccgtcc tccccgtcgt ccgattcgtc
      781 gtccgattcg tcgtcgg
//
