LOCUS       sequence-107804-         856 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  pCACS backbone (Amp), TRP1, contains an RFP flanked by BsaI sites 
            with CAGT.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 856)
  AUTHORS   Agmon N, Mitchell LA, Cai Y, Ikushima S, Chuang J, Zheng A, Choi WJ,
            Martin JA, Caravelli K, Stracquadanio G, Boeke JD
  TITLE     Yeast Golden Gate (yGG) for the Efficient Assembly of S. cerevisiae 
            Transcription Units.
  JOURNAL   ACS Synth Biol. 2015 Jul 17;4(7):853-9. doi: 10.1021/sb500372z. Epub
            2015 Mar 23.
  PUBMED    25756291
REFERENCE   2  (bases 1 to 856)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1021/sb500372z";
            journalName: "ACS Synth Biol"; date: "2015-07-17- 17"; volume: "4"; 
            issue: "7"; pages: "853-9"
COMMENT     Sequence Label: pAV10.K4
FEATURES             Location/Qualifiers
     source          1..856
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(167..841)
                     /codon_start=1
                     /gene="S. cerevisiae TRP1"
                     /product="phosphoribosylanthranilate isomerase, required 
                     for tryptophan biosynthesis"
                     /label=TRP1
                     /note="yeast auxotrophic marker"
                     /translation="MSVINFTGSSGPLVKVCGLQSTEAAECALDSDADLLGIICVPNRK
                     RTIDPVIARKISSLVKAYKNSSGTPKYLVGVFRNQPKEDVLALVNDYGIDIVQLHGDES
                     WQEYQEFLGLPVIKRLVFPKDCNILLSAASQKPHSFIPLFDSEAGGTGELLDWNSISDW
                     VGRQESPESLHFMLAGGLTPENVGDALRLNGVIGVDVSGGVETNGVKDSNKIANFVKNA
                     KK"
ORIGIN
        1 gcttacaaaa ccgacatcaa actggacatc acctcccaca acgaagacta caccatcgtt
       61 gaacagtacg aacgtgctga aggtcgtcac tccaccggtg cttaataacg ctgatagtgc
      121 tagtgtagat cgctactaga gccaggcggt ctctactggg ccggccctat ttcttagcat
      181 ttttgacgaa atttgctatt ttgttagagt cttttacacc atttgtctcc acacctccgc
      241 ttacatcaac accaataacg ccatttaatc taagcgcatc accaacattt tctggcgtca
      301 gtccaccagc taacataaaa tgtaagcttt cggggctctc ttgccttcca acccagtcag
      361 aaatcgagtt ccaatccaaa agttcacctg tcccacctgc ttctgaatca aacaagggaa
      421 taaacgaatg aggtttctgt gaagctgcac tgagtagtat gttgcagtct tttggaaata
      481 cgagtctttt aataactggc aaaccgagga actcttggta ttcttgccac gactcatctc
      541 catgcagttg gacgatatca atgccgtaat cattgaccag agccaaaaca tcctccttag
      601 gttgattacg aaacacgcca accaagtatt tcggagtgcc tgaactattt ttatatgctt
      661 ttacaagact tgaaattttc cttgcaataa ccgggtcaat tgttctcttt ctattgggca
      721 cacatataat acccagcaag tcagcatcgg aatctagagc acattctgcg gcctctgtgc
      781 tctgcaagcc gcaaactttc accaatggac cagaactacc tgtgaaatta ataacagaca
      841 tactccaagc tgcctt
//
