LOCUS       sequence-107806-         956 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Shuttle vector compatible with Flp-mediated (Flp-in) transgene 
            integration that allows for conditional cDNA and Luc expression upon
            integration. The vector contains an FRT site and two mut loxP 
            sites..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 956)
  AUTHORS   Huijbers IJ, Del Bravo J, Bin Ali R, Pritchard C, Braumuller TM, van
            Miltenburg MH, Henneman L, Michalak EM, Berns A, Jonkers J
  TITLE     Using the GEMM-ESC strategy to study gene function in mouse models.
  JOURNAL   Nat Protoc. 2015 Nov;10(11):1755-85. doi: 10.1038/nprot.2015.114. 
            Epub 2015 Oct 15.
  PUBMED    26492136
REFERENCE   2  (bases 1 to 956)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/nprot.2015.114"; journalName: "Nat Protoc"; date: 
            "2015-11"; volume: "10"; issue: "11"; pages: "1755-85"
COMMENT     Sequence Label: pFrt-invCAG-ires-Luc
FEATURES             Location/Qualifiers
     source          1..956
                     /mol_type="genomic DNA"
                     /organism="unspecified"
ORIGIN
        1 cccagaaggt accccattgt atgggatctg atctggggcc tcggtgcaca tgctttacat
       61 gtgtttagtc gaggttaaaa aacgtctagg ccccccgaac cacggggacg tggttttcct
      121 ttgaaaaaca cgatgataat atggccacaa ccatggaaga tgccaaaaac attaagaagg
      181 gcccagcgcc attctaccca ctcgaagacg ggaccgccgg cgagcagctg cacaaagcca
      241 tgaagcgcta cgccctggtg cccggcacca tcgcctttac cgacgcacat atcgaggtgg
      301 acattaccta cgccgagtac ttcgagatga gcgttcggct ggcagaagct atgaagcgct
      361 atgggctgaa tacaaaccat cggatcgtgg tgtgcagcga gaatagcttg cagttcttca
      421 tgcccgtgtt gggtgccctg ttcatcggtg tggctgtggc cccagctaac gacatctaca
      481 acgagcgcga gctgctgaac agcatgggca tcagccagcc caccgtcgta ttcgtgagca
      541 agaaagggct gcaaaagatc ctcaacgtgc aaaagaagct accgatcata caaaagatca
      601 tcatcatgga tagcaagacc gactaccagg gcttccaaag catgtacacc ttcgtgactt
      661 cccatttgcc acccggcttc aacgagtacg acttcgtgcc cgagagcttc gaccgggaca
      721 aaaccatcgc cctgatcatg aacagtagtg gcagtaccgg attgcccaag ggcgtagccc
      781 taccgcaccg caccgcttgt gtccgattca gtcatgcccg cgaccccatc ttcggcaacc
      841 agatcatccc cgacaccgct atcctcagcg tggtgccatt tcaccacggc ttcggcatgt
      901 tcaccacgct gggctacttg atctgcggct ttcgggtcgt gctcatgtac cgcttc
//
