LOCUS       sequence-107808-        5185 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  To express genes at high levels in neuronal cells. This UbC promoter
            is more active in neurons than the promoter in CMV-based vectors..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5185)
  AUTHORS   Soderblom C, Lee DH, Dawood A, Carballosa M, Jimena Santamaria A, 
            Benavides FD, Jergova S, Grumbles RM, Thomas CK, Park KK, Guest JD, 
            Lemmon VP, Lee JK, Tsoulfas P
  TITLE     3D Imaging of Axons in Transparent Spinal Cords from Rodents and 
            Nonhuman Primates
  JOURNAL   Eneuro. 2015 Mar-Apr;2(2). doi: 10.1523/ENEURO.0001-15.2015.
  PUBMED    26023683
REFERENCE   2  (bases 1 to 5185)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Eneuro. 
            2015 Mar-Apr"; volume: "2(2). doi"; pages: " 
            10.1523/ENEURO.0001-15.2015"
COMMENT     Sequence Label: pAAV-UbC
FEATURES             Location/Qualifiers
     source          1..5185
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     repeat_region   1..141
                     /label=AAV2 ITR
                     /note="inverted terminal repeat of adeno-associated virus 
                     serotype 2"
     repeat_region   12..141
                     /label=AAV2 ITR
     promoter        150..549
                     /label=UbC promoter
                     /note="human ubiquitin C promoter"
     primer_bind     1119..1138
                     /label=hUBCpro-F
                     /note="Human Ubiquitin C (UbC) promoter, forward primer"
     primer_bind     1835..1854
                     /label=pCAG-F
                     /note="Rabbit beta-globin intron, for pCAG plasmids, 
                     forward primer"
     polyA_signal    1932..2408
                     /label=hGH poly(A) signal
                     /note="human growth hormone polyadenylation signal"
     primer_bind     complement(2131..2150)
                     /label=hGH-PA-R
                     /note="Human growth hormone terminator, reverse primer"
     repeat_region   2448..2588
                     /label=AAV2 ITR
                     /note="inverted terminal repeat of adeno-associated virus 
                     serotype 2"
     repeat_region   2448..2577
                     /label=AAV2 ITR
     rep_origin      2663..3118
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(2750..2769)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     2960..2981
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(3135..3154)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     3254..3276
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(3314..3332)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        3400..3504
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(3505..3573,3574..4365)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(3723..3742)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      4536..5124
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     5025..5044
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 cctgcaggca gctgcgcgct cgctcgctca ctgaggccgc ccgggcaaag cccgggcgtc
       61 gggcgacctt tggtcgcccg gcctcagtga gcgagcgagc gcgcagagag ggagtggcca
      121 actccatcac taggggttcc tgcggccgcg gcctccgcgc cgggttttgg cgcctcccgc
      181 gggcgccccc ctcctcacgg cgagcgctgc cacgtcagac gaagggcgca gcgagcgtcc
      241 tgatccttcc gcccggacgc tcaggacagc ggcccgctgc tcataagact cggccttaga
      301 accccagtat cagcagaagg acattttagg acgggacttg ggtgactcta gggcactggt
      361 tttctttcca gagagcggaa caggcgagga aaagtagtcc cttctcggcg attctgcgga
      421 gggatctccg tggggcggtg aacgccgatg attatataag gacgcgccgg gtgtggcaca
      481 gctagttccg tcgcagccgg gatttgggtc gcggttcttg tttgtggatc gctgtgatcg
      541 tcacttggtg agtagcgggc tgctgggctg gccggggctt tcgtggccgc cgggccgctc
      601 ggtgggacgg aagcgtgtgg agagaccgcc aagggctgta gtctgggtcc gcgagcaagg
      661 ttgccctgaa ctgggggttg gggggagcgc agcaaaatgg cggctgttcc cgagtcttga
      721 atagaacctt cgctaatgcg ggaaagctct tattcgggtg agatgggctg gggcaccatc
      781 tggggaccct gacgtgaagt ttgtcactga ctggagaact cggtttgtcg tctgttgcgg
      841 gggcggcagt tatggcggtg ccgttgggca gtgcacccgt acctttggga gcgcgcgccc
      901 tcgtcgtgtc gtgacgtcac ccgttctgtt ggcttataat gcagggtggg gccacctgcc
      961 ggtaggtgtg cggtaggctt ttctccgtcg caggacgcag ggttcgggcc tagggtaggc
     1021 tctcctgaat cgacaggcgc cggacctctg gtgaggggag ggataagtga ggcgtcagtt
     1081 tctttggtcg gttttatgta cctatcttct taagtagctg aagctccggt tttgaactat
     1141 gcgctcgggg ttggcgagtg tgttttgtga agttttttag gcaccttttg aaatgtaatc
     1201 atttgggtca atatgtaatt ttcagtgtta gactagtaaa ttgtccgcta aattctggcc
     1261 gtttttggct tttttgttag acgaagctaa ggcgcgcctg agaacttcag ggtgagtttg
     1321 gggacccttg attgttcttt ctttttcgct attgtaaaat tcatgttata tggagggggc
     1381 aaagttttca gggtgttgtt tagaatggga agatgtccct tgtatcacca tgcatggacc
     1441 ctcatgataa ttttgtttct ttcactttct actctgttga caaccattgt ctcctcttat
     1501 tttcttttca ttttctgtaa ctttttcgtt aaactttagc ttgcatttgt aacgaatcta
     1561 gacaatcagg gtatattata ttgtacttca gcacagtttt agagaacaat tgttataatt
     1621 aaatgataag gtagaatatt tctgcatata aattctggct ggcgtggaaa tattcttatt
     1681 ggtagaaaca actacatcct ggtcatcatc ctgcctttct ctttatggtt acaatgatat
     1741 acactgtttg agatgaggat aaaatactct gagtccaaac cgggcccctc tgctaaccat
     1801 gttcatgcct tcttcttttt cctacagctc ctgggcaacg tgctggttat tgtgctgtct
     1861 catcaagctt ggatccgaat tcgctagcga tatctcgagt ctagagttaa cggccatatt
     1921 ggccaatcta cgggtggcat ccctgtgacc cctccccagt gcctctcctg gccctggaag
     1981 ttgccactcc agtgcccacc agccttgtcc taataaaatt aagttgcatc attttgtctg
     2041 actaggtgtc cttctataat attatggggt ggaggggggt ggtatggagc aaggggcaag
     2101 ttgggaagac aacctgtagg gcctgcgggg tctattggga accaagctgg agtgcagtgg
     2161 cacaatcttg gctcactgca atctccgcct cctgggttca agcgattctc ctgcctcagc
     2221 ctcccgagtt gttgggattc caggcatgca tgaccaggct cagctaattt ttgttttttt
     2281 ggtagagacg gggtttcacc atattggcca ggctggtctc caactcctaa tctcaggtga
     2341 tctacccacc ttggcctccc aaattgctgg gattacaggc gtgaaccact gctcccttcc
     2401 ctgtccttct gattttgtag gtaaccacgt gcggaccgag cggccgcagg aacccctagt
     2461 gatggagttg gccactccct ctctgcgcgc tcgctcgctc actgaggccg ggcgaccaaa
     2521 ggtcgcccga cgcccgggct ttgcccgggc ggcctcagtg agcgagcgag cgcgcagctg
     2581 cctgcagggg cgcctgatgc ggtattttct ccttacgcat ctgtgcggta tttcacaccg
     2641 catacgtcaa agcaaccata gtacgcgccc tgtagcggcg cattaagcgc ggcgggtgtg
     2701 gtggttacgc gcagcgtgac cgctacactt gccagcgccc tagcgcccgc tcctttcgct
     2761 ttcttccctt cctttctcgc cacgttcgcc ggctttcccc gtcaagctct aaatcggggg
     2821 ctccctttag ggttccgatt tagtgcttta cggcacctcg accccaaaaa acttgatttg
     2881 ggtgatggtt cacgtagtgg gccatcgccc tgatagacgg tttttcgccc tttgacgttg
     2941 gagtccacgt tctttaatag tggactcttg ttccaaactg gaacaacact caaccctatc
     3001 tcgggctatt cttttgattt ataagggatt ttgccgattt cggcctattg gttaaaaaat
     3061 gagctgattt aacaaaaatt taacgcgaat tttaacaaaa tattaacgtt tacaatttta
     3121 tggtgcactc tcagtacaat ctgctctgat gccgcatagt taagccagcc ccgacacccg
     3181 ccaacacccg ctgacgcgcc ctgacgggct tgtctgctcc cggcatccgc ttacagacaa
     3241 gctgtgaccg tctccgggag ctgcatgtgt cagaggtttt caccgtcatc accgaaacgc
     3301 gcgagacgaa agggcctcgt gatacgccta tttttatagg ttaatgtcat gataataatg
     3361 gtttcttaga cgtcaggtgg cacttttcgg ggaaatgtgc gcggaacccc tatttgttta
     3421 tttttctaaa tacattcaaa tatgtatccg ctcatgagac aataaccctg ataaatgctt
     3481 caataatatt gaaaaaggaa gagtatgagt attcaacatt tccgtgtcgc ccttattccc
     3541 ttttttgcgg cattttgcct tcctgttttt gctcacccag aaacgctggt gaaagtaaaa
     3601 gatgctgaag atcagttggg tgcacgagtg ggttacatcg aactggatct caacagcggt
     3661 aagatccttg agagttttcg ccccgaagaa cgttttccaa tgatgagcac ttttaaagtt
     3721 ctgctatgtg gcgcggtatt atcccgtatt gacgccgggc aagagcaact cggtcgccgc
     3781 atacactatt ctcagaatga cttggttgag tactcaccag tcacagaaaa gcatcttacg
     3841 gatggcatga cagtaagaga attatgcagt gctgccataa ccatgagtga taacactgcg
     3901 gccaacttac ttctgacaac gatcggagga ccgaaggagc taaccgcttt tttgcacaac
     3961 atgggggatc atgtaactcg ccttgatcgt tgggaaccgg agctgaatga agccatacca
     4021 aacgacgagc gtgacaccac gatgcctgta gcaatggcaa caacgttgcg caaactatta
     4081 actggcgaac tacttactct agcttcccgg caacaattaa tagactggat ggaggcggat
     4141 aaagttgcag gaccacttct gcgctcggcc cttccggctg gctggtttat tgctgataaa
     4201 tctggagccg gtgagcgtgg gtctcgcggt atcattgcag cactggggcc agatggtaag
     4261 ccctcccgta tcgtagttat ctacacgacg gggagtcagg caactatgga tgaacgaaat
     4321 agacagatcg ctgagatagg tgcctcactg attaagcatt ggtaactgtc agaccaagtt
     4381 tactcatata tactttagat tgatttaaaa cttcattttt aatttaaaag gatctaggtg
     4441 aagatccttt ttgataatct catgaccaaa atcccttaac gtgagttttc gttccactga
     4501 gcgtcagacc ccgtagaaaa gatcaaagga tcttcttgag atcctttttt tctgcgcgta
     4561 atctgctgct tgcaaacaaa aaaaccaccg ctaccagcgg tggtttgttt gccggatcaa
     4621 gagctaccaa ctctttttcc gaaggtaact ggcttcagca gagcgcagat accaaatact
     4681 gtccttctag tgtagccgta gttaggccac cacttcaaga actctgtagc accgcctaca
     4741 tacctcgctc tgctaatcct gttaccagtg gctgctgcca gtggcgataa gtcgtgtctt
     4801 accgggttgg actcaagacg atagttaccg gataaggcgc agcggtcggg ctgaacgggg
     4861 ggttcgtgca cacagcccag cttggagcga acgacctaca ccgaactgag atacctacag
     4921 cgtgagctat gagaaagcgc cacgcttccc gaagggagaa aggcggacag gtatccggta
     4981 agcggcaggg tcggaacagg agagcgcacg agggagcttc cagggggaaa cgcctggtat
     5041 ctttatagtc ctgtcgggtt tcgccacctc tgacttgagc gtcgattttt gtgatgctcg
     5101 tcaggggggc ggagcctatg gaaaaacgcc agcaacgcgg cctttttacg gttcctggcc
     5161 ttttgctggc cttttgctca catgt
//
