LOCUS       sequence-107810-         814 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Localization: Nucleus/Nucleoli, Excitation: 549, Emission: 565.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 814)
  AUTHORS   Shaner NC, Lin MZ, McKeown MR, Steinbach PA, Hazelwood KL, Davidson 
            MW, Tsien RY
  TITLE     Improving the photostability of bright monomeric orange and red 
            fluorescent proteins.
  JOURNAL   Nat Methods. 2008 Jun;5(6):545-51. doi: 10.1038/nmeth.1209. Epub 
            2008 May 4.
  PUBMED    18454154
REFERENCE   2  (bases 1 to 814)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/nmeth.1209"; journalName: "Nat Methods"; date: "2008-06"; 
            volume: "5"; issue: "6"; pages: "545-51"
COMMENT     Sequence Label: mOrange2-Fibrillarin-7
FEATURES             Location/Qualifiers
     source          1..814
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      47..56
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             53..760
                     /codon_start=1
                     /product="photostable monomeric orange derivative of DsRed 
                     fluorescent protein (Shaner et al., 2008)"
                     /label=mOrange2
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEENNMAIIKEFMRFKVRMEGSVNGHEFEIEGEGEGRPYEG
                     FQTAKLKVTKGGPLPFAWDILSPHFTYGSKAYVKHPADIPDYFKLSFPEGFKWERVMNY
                     EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK
                     GKIKMRLKLKDGGHYTSEVKTTYKAKKPVQLPGAYIVDIKLDITSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
ORIGIN
        1 gcagagctgg tttagtgaac cgtcagatcc gctagcgcta ccggtcgcca ccatggtgag
       61 caagggcgag gagaataaca tggccatcat caaggagttc atgcgcttca aggtgcgcat
      121 ggagggctcc gtgaacggcc acgagttcga gatcgagggc gagggcgagg gccgccccta
      181 cgagggcttt cagaccgcta agctgaaggt gaccaagggt ggccccctgc ccttcgcctg
      241 ggacatcctg tcccctcatt tcacctacgg ctccaaggcc tacgtgaagc accccgccga
      301 catccccgac tacttcaagc tgtccttccc cgagggcttc aagtgggagc gcgtgatgaa
      361 ctacgaggac ggcggcgtgg tgaccgtgac ccaggactcc tccctgcagg acggcgagtt
      421 catctacaag gtgaagctgc gcggcaccaa cttcccctcc gacggccccg tgatgcagaa
      481 gaagaccatg ggctgggagg cctcctccga gcggatgtac cccgaggacg gtgccctgaa
      541 gggcaagatc aagatgaggc tgaagctgaa ggacggcggc cactacacct ccgaggtcaa
      601 gaccacctac aaggccaaga agcccgtgca gctgcccggc gcctacatcg tcgacatcaa
      661 gttggacatc acctcccaca acgaggacta caccatcgtg gaacagtacg aacgcgccga
      721 gggccgccac tccaccggcg gcatggacga gctgtacaag tccggactca gatctcgagc
      781 catgaagcca ggattcagtc cccgtggggg tggc
//
