LOCUS       sequence-107815-         225 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Tet inducible scrambled shRNA..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 225)
  AUTHORS   Rudin CM, Durinck S, Stawiski EW, Poirier JT, Modrusan Z, Shames DS,
            Bergbower EA, Guan Y, Shin J, Guillory J, Rivers CS, Foo CK, Bhatt 
            D, Stinson J, Gnad F, Haverty PM, Gentleman R, Chaudhuri S, 
            Janakiraman V, Jaiswal BS, Parikh C, Yuan W, Zhang Z, Koeppen H, Wu 
            TD, Stern HM, Yauch RL, Huffman KE, Paskulin DD, Illei PB, 
            Varella-Garcia M, Gazdar AF, de Sauvage FJ, Bourgon R, Minna JD, 
            Brock MV, Seshagiri S
  TITLE     Comprehensive genomic analysis identifies SOX2 as a frequently 
            amplified gene in small-cell lung cancer.
  JOURNAL   Nat Genet. 2012 Oct;44(10):1111-6. doi: 10.1038/ng.2405. Epub 2012 
            Sep 2.
  PUBMED    22941189
REFERENCE   2  (bases 1 to 225)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1038/ng.2405"; 
            journalName: "Nat Genet"; date: "2012-10"; volume: "44"; issue: 
            "10"; pages: "1111-6"
COMMENT     Sequence Label: Tet-pLKO-puro-Scrambled
FEATURES             Location/Qualifiers
     source          1..225
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        60..154
                     /label=H1-2O2 promoter
                     /note="doxycycline-inducible variant of the human H1 RNA 
                     promoter (Henriksen et al., 2007)"
     primer_bind     71..90
                     /label=H1
                     /note="Human H1 promoter, forward primer"
     protein_bind    108..126
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    136..154
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
ORIGIN
        1 ttcgattagt gaacggatct cgacggtatc gatcacgaga ctagcctcga gcggccgcaa
       61 tatttgcatg tcgctatgtg ttctgggaaa tcaccataaa cgtgaaatcc ctatcagtga
      121 tagagactta taagttccct atcagtgata gagacaccgg cctaaggtta agtcgccctc
      181 gctcgagcga gggcgactta accttaggtt tttaattctc gacct
//
