LOCUS       sequence-107816-         596 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Shuttle vector compatible with Flp-mediated (Flp-in) transgene 
            integration that allows for conditional cDNA and Luc expression upon
            integration. The vector contains an FRT site and two mut loxP 
            sites..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 596)
  AUTHORS   Huijbers IJ, Del Bravo J, Bin Ali R, Pritchard C, Braumuller TM, van
            Miltenburg MH, Henneman L, Michalak EM, Berns A, Jonkers J
  TITLE     Using the GEMM-ESC strategy to study gene function in mouse models.
  JOURNAL   Nat Protoc. 2015 Nov;10(11):1755-85. doi: 10.1038/nprot.2015.114. 
            Epub 2015 Oct 15.
  PUBMED    26492136
REFERENCE   2  (bases 1 to 596)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/nprot.2015.114"; journalName: "Nat Protoc"; date: 
            "2015-11"; volume: "10"; issue: "11"; pages: "1755-85"
COMMENT     Sequence Label: pFrt-invCAG-ires-Luc
FEATURES             Location/Qualifiers
     source          1..596
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    184..217
                     /label=lox71
                     /bound_moiety="Cre recombinase"
                     /note="Left element (LE) mutant of loxP (Araki et al., 
                     2010). Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     polyA_signal    219..353
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
ORIGIN
        1 gctcgtcaag aagacagggc caggtttccg ggccctcaca ttgccaaaag acggcaatat
       61 ggtggaaaat aacatataga caaacgcaca ccggccttat tccaagcggc ttcggccagt
      121 aacgttaggg gggggggagg gagaggggcg gtttaaacag ggtagatctt gggaggccgg
      181 cctataactt cgtataatgt atgctatacg aacggtagga tccagacatg ataagataca
      241 ttgatgagtt tggacaaacc acaactagaa tgcagtgaaa aaaatgcttt atttgtgaaa
      301 tttgtgatgc tattgcttta tttgtaacca ttataagctg caataaacaa gttaacaaca
      361 acaattgcat tcattttatg tttcaggttc agggggaggt gtgggaggtt ttttaaagca
      421 agtaaaacct ctacaaatgt ggtatggctg attatgatcg ctagatccag acatgataag
      481 atacattgat gagtttggac aaaccacaac tagaatgcag tgaaaaaaat gctttatttg
      541 tgaaatttgt gatgctattg ctttatttgt aaccattata agctgcaata aacaag
//
