LOCUS       sequence-107818-         913 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Localization: Mitochondria, Excitation: 462, Emission: 492.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 913)
  AUTHORS   Ai HW, Olenych SG, Wong P, Davidson MW, Campbell RE
  TITLE     Hue-shifted monomeric variants of Clavularia cyan fluorescent 
            protein: identification of the molecular determinants of color and 
            applications in fluorescence imaging.
  JOURNAL   BMC Biol. 2008 Mar 6;6:13. doi: 10.1186/1741-7007-6-13.
  PUBMED    18325109
REFERENCE   2  (bases 1 to 913)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "BMC Biol.";
            date: "2008-03-6"; volume: "6:13. doi"; pages: " 
            10.1186/1741-7007-6-13"
COMMENT     Sequence Label: mTFP1-Mito-7
FEATURES             Location/Qualifiers
     source          1..913
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             39..113
                     /codon_start=1
                     /product="mitochondrial presequence of human cytochrome c 
                     oxidase subunit VIII (Rizutto et al., 1989)"
                     /label=COX8 presequence
                     /translation="MSVLTPLLLRGLTGSARRLPVPRAK"
     regulatory      141..150
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             147..857
                     /codon_start=1
                     /product="monomeric teal (cyan) fluorescent protein (Ai et 
                     al., 2006)"
                     /label=mTFP1
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEETTMGVIKPDMKIKLKMEGNVNGHAFVIEGEGEGKPYDG
                     TNTINLEVKEGAPLPFSYDILTTAFAYGNRAFTKYPDDIPNYFKQSFPEGYSWERTMTF
                     EDKGIVKVKSDISMEEDSFIYEIHLKGENFPPNGPVMQKKTTGWDASTERMYVRDGVLK
                     GDVKHKLLLEGGGHHRVDFKTIYRAKKAVKLPDYHFVDHRIEILNHDKDYNKVTVYESA
                     VARNSTDGMDELYK"
ORIGIN
        1 tagcagagct ggtttagtga accgtcagat ccgctagcat gtccgtcctg acgccgctgc
       61 tgctgcgggg cttgacaggc tcggcccggc ggctcccagt gccgcgcgcc aagatccatt
      121 cgttggggga tccaccggtc gccaccatgg tgagcaaggg cgaggagacc acaatgggcg
      181 taatcaagcc cgacatgaag atcaagctga agatggaggg caacgtgaat ggccacgcct
      241 tcgtgatcga gggcgagggc gagggcaagc cctacgacgg caccaacacc atcaacctgg
      301 aggtgaagga gggagccccc ctgcccttct cctacgacat tctgaccacc gcgttcgcct
      361 acggcaacag ggccttcacc aagtaccccg acgacatccc caactacttc aagcagtcct
      421 tccccgaggg ctactcttgg gagcgcacca tgaccttcga ggacaagggc atcgtgaagg
      481 tgaagtccga catctccatg gaggaggact ccttcatcta cgagatacac ctcaagggcg
      541 agaacttccc ccccaacggc cccgtgatgc agaagaagac caccggctgg gacgcctcca
      601 ccgagaggat gtacgtgcgc gacggcgtgc tgaagggcga cgtcaagcac aagctgctgc
      661 tggagggcgg cggccaccac cgcgttgact tcaagaccat ctacagggcc aagaaggcgg
      721 tgaagctgcc cgactatcac tttgtggacc accgcatcga gatcctgaac cacgacaagg
      781 actacaacaa ggtgaccgtt tacgagagcg ccgtggcccg caactccacc gacggcatgg
      841 acgagctgta caagtaagcg gccgcgactc tagatcataa tcagccatac cacatttgta
      901 gaggttttac ttg
//
