LOCUS       sequence-10782-e         841 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 841)
  AUTHORS   Johannessen CM, Boehm JS, Kim SY, Thomas SR, Wardwell L, Johnson LA,
            Emery CM, Stransky N, Cogdill AP, Barretina J, Caponigro G, 
            Hieronymus H, Murray RR, Salehi-Ashtiani K, Hill DE, Vidal M, Zhao 
            JJ, Yang X, Alkan O, Kim S, Harris JL, Wilson CJ, Myer VE, Finan PM,
            Root DE, Roberts TM, Golub T, Flaherty KT, Dummer R, Weber BL, 
            Sellers WR, Schlegel R, Wargo JA, Hahn WC, Garraway LA
  TITLE     COT drives resistance to RAF inhibition through MAP kinase pathway 
            reactivation.
  JOURNAL   Nature. 2010 Dec 16;468(7326):968-72. doi: 10.1038/nature09627
  PUBMED    21107320
REFERENCE   2  (bases 1 to 841)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10"; journalName: 
            "Nature"; date: "2010-12-16- 16"; volume: "468"; issue: "7326"; 
            pages: "968-72"
COMMENT     Sequence Label: pDONR223-PAK1
FEATURES             Location/Qualifiers
     source          1..841
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    30..129
                     /gene="mutant version of attL"
                     /label=attL1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     CDS             complement(664..681)
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
ORIGIN
        1 ggggggggcg gcgcctgaaa atagccgggc ccaatatgat tttattttga ctgatagtga
       61 cctgttcgtt gcaacaaatt gatgagcaat gcttttttat aatgccaact ttgtacaaaa
      121 aagttggcac catgtcaaat aacggcctag acattcaaga caaaccccca gcccctccga
      181 tgagaaatac cagcactatg attggagccg gcagcaaaga tgctggaacc ctaaaccatg
      241 gttctaaacc tctgcctcca aacccagagg agaagaaaaa gaaggaccga ttttaccgat
      301 ccattttacc tggagataaa acaaataaaa agaaagagaa agagcggcca gagatttctc
      361 tcccttcaga ttttgaacac acaattcatg tcggttttga tgctgtcaca ggggagttta
      421 cgggaatgcc agagcagtgg gcccgcttgc ttcagacatc aaatatcact aagtcggagc
      481 agaagaaaaa cccgcaggct gttctggatg tgttggagtt ttacaactcg aagaagacat
      541 ccaacagcca gaaatacatg agctttacag ataagtcagc tgaggattac aattcttcta
      601 atgccttgaa tgtgaaggct gtgtctgaga ctcctgcagt gccaccagtt tcagaaaatg
      661 aggatgatga tgatgatgat gctaccccac caccagtgat tgctccacgc ccagagcaca
      721 caaaatctgt atacacacgg tctgtgattg aaccacttcc tgtcactcca actcgggaag
      781 tggctacctc ctccttttca ccctctgaaa attacaccca ctcccccaga aggtttggac
      841 c
//
