LOCUS       sequence-107820-         833 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  pUC19 backbone (Amp), contains an RFP flanked by BsaI sites with 
            CAGT.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 833)
  AUTHORS   Agmon N, Mitchell LA, Cai Y, Ikushima S, Chuang J, Zheng A, Choi WJ,
            Martin JA, Caravelli K, Stracquadanio G, Boeke JD
  TITLE     Yeast Golden Gate (yGG) for the Efficient Assembly of S. cerevisiae 
            Transcription Units.
  JOURNAL   ACS Synth Biol. 2015 Jul 17;4(7):853-9. doi: 10.1021/sb500372z. Epub
            2015 Mar 23.
  PUBMED    25756291
REFERENCE   2  (bases 1 to 833)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1021/sb500372z";
            journalName: "ACS Synth Biol"; date: "2015-07-17- 17"; volume: "4"; 
            issue: "7"; pages: "853-9"
COMMENT     Sequence Label: pAV10
FEATURES             Location/Qualifiers
     source          1..833
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     complement(204..221)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(204..220)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(213..235)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      362..817
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(449..468)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     659..680
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
ORIGIN
        1 agctgccggg tgcttacaaa accgacatca aactggacat cacctcccac aacgaagact
       61 acaccatcgt tgaacagtac gaacgtgctg aaggtcgtca ctccaccggt gcttaataac
      121 gctgatagtg ctagtgtaga tcgctactag agccaggcgg tctcatttta gcggccgcgg
      181 gccggcctac cgagctcgaa ttcactggcc gtcgttttac aacgtcgtga ctgggaaaac
      241 cctggcgtta cccaacttaa tcgccttgca gcacatcccc ctttcgccag ctggcgtaat
      301 agcgaagagg cccgcaccga tcgcccttcc caacagttgc gcagcctgaa tggcgaatgg
      361 gacgcgccct gtagcggcgc attaagcgcg gcgggtgtgg tggttacgcg cagcgtgacc
      421 gctacacttg ccagcgccct agcgcccgct cctttcgctt tcttcccttc ctttctcgcc
      481 acgttcgccg gctttccccg tcaagctcta aatcgggggc tccctttagg gttccgattt
      541 agtgctttac ggcacctcga ccccaaaaaa cttgattagg gtgatggttc acgtagtggg
      601 ccatcgccct gatagacggt ttttcgccct ttgacgttgg agtccacgtt ctttaatagt
      661 ggactcttgt tccaaactgg aacaacactc aaccctatct cggtctattc ttttgattta
      721 taagggattt tgccgatttc ggcctattgg ttaaaaaatg agctgattta acaaaaattt
      781 aacgcgaatt ttaacaaaat attaacgctt acaatttagg tggcactttt cgg
//
