LOCUS       sequence-107825-         954 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Localization: Focal Adhesions, Excitation: 587, Emission: 610.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 954)
  TITLE     Davidson lab - mCherry constructs
REFERENCE   2  (bases 1 to 954)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: mCherry-Lasp1-N-10
FEATURES             Location/Qualifiers
     source          1..954
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      44..53
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     regulatory      664..673
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     promoter        complement(895..946)
                     /label=CMVd2 promoter
                     /note="truncated human cytomegalovirus (CMV) immediate 
                     early promoter that yields moderately reduced expression 
                     relative to the full-length promoter"
     primer_bind     complement(924..944)
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
ORIGIN
        1 aactccttga tgatggccat gttatcctcc tcgcccttgc tcaccatggt ggcgaccggt
       61 ggatcccctc cggagctgat ggcctccacg tagttggccg gcagcatccc cgtgtcgccg
      121 gtgcgctcca ccgtcccgta catccagccg tcgtcgatct gctgcacgtt gacgatggtg
      181 tccccgtcct ggaaggagac ctcgtcctcg tcggcggcgc tgtagtcata caccacgcgg
      241 taccgcttcc cgccaccacc tggggcgctg cgctgtatgg agactggggc tgcaggctcc
      301 ttgtagccac cataggactg ggccaccggc tgctgctggg gctgctggta aaccggggca
      361 ctggtcggga tgtggtgagg ctgctgctgc tccaggggcc gccggtagct gctgccgtcc
      421 tgtgaatccc gacgctctgg ctccatgccc tcgcccccgc tagggcccat gcggctcttc
      481 tcaaactcct catggtattt tatgttactg atctggtcct gggtcttctt gattctctgg
      541 agctcgggcg tgtctgccac tacgctgaaa cctttgccct tgttcttctc aaactcctcc
      601 ttgtagcgca cctgactctg gagctcactc tgttgcttga ggcgaaggtt ttccggggtg
      661 tccgccacca tggtgaagga ctgcttgggg tagtgtgcgt tgcagtaggg cttcttctcg
      721 tagcccttgt agttcttcat gttcagtgtc atcttgcagg tctcgcaatg gaagcatgct
      781 ttatgccaga acttatccag acagttcacc ttctccgtgg gatacacgat cttgccgcac
      841 cgggcgcagt tggggttcat ggtggcgcta gcggatctga cggttcacta aaccagctct
      901 gcttatatag acctcccacc gtacacgcct accgcccatt tgcgtcaatg gggc
//
